Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.
Type:
Grant
Filed:
November 22, 2017
Date of Patent:
January 14, 2020
Assignee:
Celsee Diagnostics, Inc.
Inventors:
Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri, Christopher Siemer
Abstract: Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.
Type:
Grant
Filed:
February 22, 2016
Date of Patent:
January 7, 2020
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Thomas M. Baer, Jennifer R. Cochran, Bob Chen, Spencer Caleb Alford, Ivan Dimov
Abstract: The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids in a process referred to herein as “expansion sequencing” (ExSEQ).
Type:
Grant
Filed:
October 20, 2017
Date of Patent:
January 7, 2020
Assignees:
Massachusetts Institute of Technology, President and Fellows of Harvard College
Inventors:
Fei Chen, Shahar Alon, Andrew Payne, Asmamaw Wassie, Daniel Goodwin, Edward Stuart Boyden, Evan Daugharthy, Jonathan Scheiman
Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
Abstract: Microarray compositions suitable for analysis by one or several spectrographic methods are disclosed. In an embodiment, a microarray composition includes a three-dimensional solid support and a plurality of reactive microbeads positioned on the solid support in spatially distinct and addressable locations.
Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
Abstract: This disclosure describes, in one aspect, a method of amplifying an RNA template. Generally, the method includes synthesizing an oligonucleotide from the RNA template, isolating at least a portion of the oligonucleotide, and subjecting the isolated product to treatment with an RNase and/or glycosylase.
Type:
Grant
Filed:
December 2, 2014
Date of Patent:
October 1, 2019
Assignee:
REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventors:
Praveensingh B. Hajeri, Subbaya Subramanian
Abstract: The present invention relates to methods of screening libraries of chimeric molecules comprising ribotoxic polypeptides, where screening is based on the interim reduction or elimination of ribotoxicity and the methods can identify cytotoxic molecules, each comprising a binding region and a ribotoxic region which jointly possess a desired assay-selectable characteristic, such as, e.g., binding to a target biomolecule, binding to a target cell, and/or cellular internalization.
Type:
Grant
Filed:
February 4, 2015
Date of Patent:
September 24, 2019
Assignee:
MOLECULAR TEMPLATES, INC.
Inventors:
Eric Poma, Erin Willert, Jason Kim, Jack Higgins
Abstract: A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.
Abstract: The invention provides novel peptide binders for streptavidin (SA), Taq polymerase and several human proteins: Prostate Specific Antigen (PSA), thrombin, Tumor Necrosis Factor Alpha (TNF?), and Urokinase-type Plasminogen Activator (uPA).
Type:
Grant
Filed:
April 19, 2016
Date of Patent:
August 27, 2019
Assignee:
ROCHE SEQUENCING SOLUTIONS, INC.
Inventors:
Thomas Albert, Ryan Bannen, Victor Lyamichev, Jigar Patel, Eric Sullivan
Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
Type:
Grant
Filed:
April 29, 2019
Date of Patent:
August 27, 2019
Assignee:
10X GENOMICS, INC.
Inventors:
Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
Abstract: Apparatus and methods for determining whether a test compound induces cell activity, changes cell activity, prevents cell activity, or inhibits cell activity. An embodiment comprises placing a test compound solution in contact with a cell suspension media containing cells, diffusing the test compound solution into the cell suspension from one or more sides, and detecting activity in the cells with respect to their distance from the side from which the test compound is diffusing. Embodiments may provide an apparatus that allows a side source, a point source, or both, from which a test compound solution diffuses into a cell suspension media and contacts cells. Detecting cell activity may involve detecting activity in a first cell group proximate to the side from which the test compound is diffusing, and detecting activity in a second cell group farther than the first cell group from the side from which the test compound is diffusing.
Abstract: Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.
Type:
Grant
Filed:
February 22, 2016
Date of Patent:
August 6, 2019
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Jennifer R. Cochran, Thomas M. Baer, Bob Chen, Spencer Caleb Alford, Arvind Kannan, Sungwon Lim, Ivan Dimov
Abstract: The present invention relates to a method and tools for extracting information on a compounds influence on a cellular phenotype. The method of the invention may be used as a very efficient procedure for testing the efficacy or resistance of single drugs or combinations of drugs on cells from individual patients. Thus, the methods may be useful for predicting efficacy of a drug on a given patient. The methods are also useful for testing of compounds for toxicity, identifying drug targets for known or novel compounds.
Abstract: This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.
Type:
Grant
Filed:
January 3, 2018
Date of Patent:
July 23, 2019
Assignee:
TAXON BIOSCIENCES, INC.
Inventors:
Matthew Ashby, Ladonna Wood, Ulrika Lidstrom, Christine Clarke, Alison Gould, Dariusz Strapoc, Adewale J. Lambo, Bradley James Huizinga
Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
Type:
Grant
Filed:
October 16, 2014
Date of Patent:
July 16, 2019
Assignee:
LIFE TECHNOLOGIES CORPORATION
Inventors:
George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
Type:
Grant
Filed:
February 21, 2019
Date of Patent:
July 2, 2019
Assignee:
10X GENOMICS, INC.
Inventors:
Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
Abstract: A bead immobilization method including receiving at least one bead in at least one well in a casting surface, and casting a casting material over the casting surface to form a reverse casting in which the at least one bead is cast onto at least one post upstanding from a surface of the reverse casting.
Type:
Grant
Filed:
March 2, 2017
Date of Patent:
July 2, 2019
Assignee:
SWINBURNE UNIVERSITY OF TECHNOLOGY
Inventors:
Dan Veniamin Nicolau, Luisa Filipponi, Prashant Dnyandeo Sawant
Abstract: Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity.