Abstract: The present inventors focused on siE sequences that have been thought to show RNAi activity against HCV viral RNAs, and mainly selected the D5-50 and D5-197 regions present within the IRES region, and carried on the analysis. As a result, the present inventors successfully identified siRNA sequences that exhibit a more effective RNAi activity against hepatitis C virus RNAs. Furthermore, the siRNAs were demonstrated to have a significant inhibitory effect on HCV propagation in an in vivo system.
Type:
Grant
Filed:
November 26, 2009
Date of Patent:
February 17, 2015
Assignees:
Chugai Seiyaku Kabushiki Kaisha, Tokyo Metropolitan Institute of Medical Science
Abstract: Disclosed herein are antisense compounds and methods for selectively of reducing expression of an allelic variant of a gene containing a single nucleotide polymorphism (SNP). Such methods, compounds, and composition are useful to treat, prevent, or ameliorate diseases, including neurodegenerative, such as Huntington's Disease (HD).
Type:
Grant
Filed:
February 8, 2011
Date of Patent:
February 17, 2015
Assignee:
Isis Pharmaceuticals, Inc.
Inventors:
C. Frank Bennett, Susan M. Freier, Sarah Greenlee, Eric E. Swayze
Abstract: The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.
Type:
Grant
Filed:
March 20, 2013
Date of Patent:
February 3, 2015
Assignee:
Arizona Board of Regents, a Body Corporate of the State of Arizona Acting for and on Behalf of Arizona State University
Abstract: Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
Type:
Grant
Filed:
December 5, 2012
Date of Patent:
February 3, 2015
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Keith Bauer, Thomas W. Myers, Shawn Suko
Abstract: The present application relates at least in part to methods for the administration of small interfering RNAs (siRNAs) to the spinal cord of a human or animal patient and also to a method of treatment for spinal cord injury and other diseases and disorders of the CNS. In particular, the application discloses methods to deliver an siRNA compound locally, directly and without the need for transduction vehicles and formulations in effective doses to the injured spinal cord to promote recovery of CNS function and or attenuation of allodynia.
Type:
Grant
Filed:
November 8, 2010
Date of Patent:
December 2, 2014
Assignees:
Quark Pharmaceuticals, Inc., Rutgers, The State University of New Jersey
Abstract: Described herein is a mitochondrial-targeted RNA expression system (mtTRES) for delivery of RNA molecules to mitochondria. mtTRES vectors generate RNAs in vivo that are un-capped, non-polyadenylated, and actively directed to mitochondria. The disclosed vectors are capable of delivering either non-coding RNA molecules or RNA molecules encoding a protein of interest to the mitochondria. In particular, the disclosed vectors include (1) an RNAPIII initiation (promoter) sequence, (2) a non-coding leader sequence (NCL), (3) a mitochondrial translation initiation sequence and an ORF encoding a protein of interest, or a sequence encoding a non-coding RNA, and (4) an RNAPIII termination sequence.
Type:
Grant
Filed:
April 10, 2013
Date of Patent:
November 11, 2014
Assignee:
University of Pittsburgh—Of the Commonwealth System of Higher Education
Inventors:
Michael J. Palladino, Alicia M. Palladino
Abstract: The disclosure provides compositions and methods for treating an ovarian cancer in a subject. More specifically, the disclosure provides microRNA (miRNA) inhibitor molecules that target to different miRNAs for treating different types of ovarian cancers in a subject. Furthermore, different modifications of miRNA inhibitor molecules as well as different derivatives of miRNA inhibitor molecules are also described.
Abstract: The present invention provides compositions and methods for the delivery of interfering RNAs such as siRNAs that silence APOB expression in cells such as liver cells. In particular, the nucleic acid-lipid particles provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells such as liver cells in vivo. The compositions of the present invention are highly potent, thereby allowing effective knock-down of APOB at relatively low doses. In addition, the compositions and methods of the present invention are less toxic and provide a greater therapeutic index compared to compositions and methods previously known in the art.
Type:
Grant
Filed:
May 12, 2011
Date of Patent:
October 21, 2014
Assignee:
Protiva Biotherapeutics, Inc.
Inventors:
James Heyes, Mark Wood, Alan Martin, Amy C. H. Lee, Adam Judge, Marjorie Robbins, Ian MacLachlan
Abstract: This invention relates to compounds, compositions, and methods useful for reducing ?-catenin target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents.
Abstract: The presently-disclosed subject matter includes methods of identifying an Alu RNA inhibitor, and methods and compositions for inhibiting Alu RNA. Methods and compositions can be used for the treatment of geographic atrophy and other conditions of interest.
Type:
Grant
Filed:
June 1, 2011
Date of Patent:
August 19, 2014
Assignee:
University of Kentucky Research Foundation
Abstract: An object is to provide a cell growth inhibitor also effective for androgen-independent prostate cancer. The present invention provides a cell growth inhibitor having, as an active ingredient, an expression inhibitor or function inhibitor of an antisense RNA (CTBP1-AS) expressed in the vicinity of an androgen receptor (AR) binding site of a C-terminal binding protein (CTBP1) gene.
Abstract: The invention relates to modified siRNA compounds which down-regulate target gene expression, to pharmaceutical compositions comprising such compounds and to methods of treating and/or preventing the incidence or severity of various diseases or conditions associated with the genes and/or symptoms associated with such diseases or conditions.
Abstract: The present invention relates to non-invasive methods for treating diseases, disorders and injury to the central nervous system (CNS), and in particular to otic compositions and to methods of use thereof.
Type:
Grant
Filed:
December 9, 2010
Date of Patent:
July 15, 2014
Assignee:
Quark Pharmaceuticals, Inc.
Inventors:
Elena Feinstein, Igor Spivak, Evgenia Alpert, Ron Lahav
Abstract: Provided are an aptamer having an inhibitory activity on FGF2; a complex containing an aptamer having a binding activity or an inhibitory activity on FGF2, and a functional substance (e.g., affinity substance, labeling substance, enzyme, drug delivery vehicle, or drug and the like); a medicament, diagnostic reagent or label containing an aptamer having a binding activity or an inhibitory activity on FGF2, or a complex containing said aptamer and a functional substance; and the like.
Abstract: The present invention relates to novel compositions and therapeutic methods for the treatment of cancer, in particular malignant glioma. The compositions include antisense oligonucleotides or RNAs or vectors encoding them which reduce expression of downregulated in renal cell carcinoma (DRR) in tumor cells, and inhibit malignant glioma cell invasion.
Type:
Grant
Filed:
May 18, 2011
Date of Patent:
July 1, 2014
Inventors:
Kevin Petrecca, Masad Damha, Glen Francis Deleavey
Abstract: The present invention concerns Sipa1l1, a new target involved in adipogenesis modulation. Using a siRNA approach, the inventors demonstrated that decrease in Sipa1l1 activity in preadipocytes and adipose tissue induces a decrease in adipogenesis. Thus, the present invention relates to modulators of Sipa1l1 activity as well as screening test for identification of modulators of the activity of this target, and their use, especially in pharmaceutical composition, to modulate adipogenesis and thus treat obesity and related disorders.
Type:
Grant
Filed:
October 1, 2010
Date of Patent:
June 24, 2014
Assignee:
Sanofi
Inventors:
Diana Hall, Maria Jimenez, Carine Poussin, Bernard Thorens
Abstract: Inhibitors that can inhibit expression of FAM3B gene to reduce the levels of expression products, or can combine the expression products to reduce the activity of promoting lipid synthesis of FAM3B gene product are provided, wherein the inhibitors are one or more inhibitors selected from the group consisting of small interfering RNAs, antisense oligonucleotides, antibodies against FAM3B proteins and active organic compounds. Cells, vectors or inhibitor compositions, comprising such inhibitors, methods for inhibiting expression of FAM3B gene or inhibiting the activity of promoting lipid synthesis of FAM3B gene product using the inhibitors are provided. Methods for treating diseases mediated by expression of FAM3B gene using such inhibitors and uses of the inhibitors in preparing pharmaceuticals for preventing and/or treating the disease mediated by FAM3B gene expression are also provided.
Abstract: The present invention relates to an siRNA inhibiting expression of the OTUB1 protein and a composition for preventing or treating cancer containing same as an active ingredient. In accordance with the present invention, cancer cell growth can be remarkably inhibited by inhibiting OTUB1 expression using the siRNA of the present invention.
Type:
Grant
Filed:
February 6, 2013
Date of Patent:
June 10, 2014
Assignee:
Korean Institute of Science and Technology
Abstract: The present invention discloses multiple treatment regimens for vascular-related diseases and disorders. The present invention provides for methods of treating vascular-related disorders based on gene expression studies from samples collected from individuals having symptoms of vascular-related disorders. Additionally, methods are disclosed involving diagnostic techniques to focus treatment regimens. Finally, methods of treating vascular-related disorder involving targeting microRNAs are also disclosed.