Abstract: The present description provides methods, assays and reagents useful for sequencing proteins. Sequencing proteins in a broad sense involves observing the plausible identity and order of amino acids, which is useful for sequencing single polypeptide molecules or multiple molecules of a single polypeptide. In one aspect, the methods are useful for sequencing multiple polypeptides. The methods and reagents described herein can be useful for high resolution interrogation of the proteome and enabling ultrasensitive diagnostics critical for early detection of diseases.

Abstract: The sample preparation apparatus includes a measurement unit configured to measure a sample containing particles acquired from a sample container and detect measurement target particles in the sample, a sample preparation unit capable of adjusting the concentration of measurement target particles in a sample acquired from a sample container and configured to prepare a measurement sample by mixing a sample and any of a plurality of types of particle detection reagents including a particle labeling substance, and a control unit for controlling the sample preparation unit so as to generate concentration information of the measurement target particles in the sample in the sample container based on the measurement data of the measurement unit and adjust the concentration of the measurement target particles in the sample acquired from the sample container according to the generated concentration information and the type of particle detection reagent used for preparing the measurement sample.

Abstract: A sliding removable coaxial capillary microfluidic chip and a preparation method therefor. The chip comprises a substrate which has a hole as a window; an alignment platform I and II are respectively fixed on both sides of the window, and the alignment platform I is internally provided with a circular groove I and a square groove I formed successively on the same axis; the alignment platform II is internally provided with a circular groove II and a square groove II formed successively on the same axis; both ends of a square capillary are respectively placed in the square groove I and II, one circular capillary enters the square groove I through the circular groove I, one end of another circular capillary is placed in a sliding platform, and the other end slides through the sliding platform and enters the square groove II through the circular groove II.

Abstract: A method for assessing an effect, such as a treatment, disease, or passage of time on a cellular unit of an organism includes providing a sample including a cellular unit subject to the effect in a supporting medium in a hermetically sealed container. The container is deoxygenated to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the medium. Values for variables associated with the medium and/or the cellular unit are determined at each of a plurality of different times. Various combinations of the predetermined amount of hypoxia, the predetermined rate of oxygen consumption, the values for the variables associated with the medium and/or cellular unit, and the times and then correlated to generate a multi-dimensional surface. This surface is compared with another surface correlating corresponding values relating to another cellular unit such as one not subject to the effect.

Abstract: A method for in vitro simulation and evaluation of platelet adhesion in blood-contacting medical devices is disclosed, including the following steps: (1) using a glycerin aqueous solution with a mass percentage concentration of 40% in an extracorporeal circulation circuit to simulate a viscosity and hydrodynamic characteristics of blood, and adding fluorescent particles with a diameter of 3 ?m to 5 ?m to the solution to simulate platelets; (2) after the solution circulates in the circuit for a specified time period, removing flow passage components of a tested device, and observing the deposition of the fluorescent particles on a blood-contacting surface inside the device by naked eyes and photographs; and (3) using laser-induced fluorescence (LIF) technique to apply laser light on a device surface deposited with the fluorescent particles and in contact with blood, and using charge-coupled device (CCD) camera imaging to photograph the aggregation and adhesion of laser-induced fluorescent particles.

Abstract: This disclosure provides devices and methods for the isolation of single cells or particles of interest from a solution comprising a plurality of cells or a solution composed of a homogenous population of particles. Specifically, the present disclosure is directed to microfluidic devices and methods for analyzing cells in a sample. More specifically, the present disclosure provides droplet microfluidic devices and methods for using the same to obtain (trap), encapsulate, and retrieve (isolate) single cells or particles from a sample with improved efficiency.

Abstract: According to an example aspect of the present invention, there is provided an apparatus comprising a volume for receiving a gas sample; and an ultrasonic transducer; wherein the ultrasonic transducer is caused to generate a standing wave to the volume, said standing wave comprising at least one particle trapping zone for trapping particles carried by the gas sample, and to release particles trapped to the at least particle trapping zone by decreasing power of the standing wave to at least one lower power level and/or switching off the standing wave.

Abstract: The present invention is an exosome isolation method for obtaining high purity exosomes inexpensively from samples of large and small amounts and types of samples from which exosomes could not be obtained before such as plant lysates. The exosome isolation method incorporates a two phase fluid extraction system, and includes the steps of isolation using dextran and PEG for removing proteins and undesired substances therein, and the steps of washing and dextran removal to collect exosomes in a pure form.

Abstract: This present invention provides means for cell harvesting and isolation to be carried out with a single apparatus. This represents a more convenient way for the processing of cells compared with known methods and represents a useful development for the manufacture of cell therapies.

Abstract: A first aspect of the present invention is directed to a method for the detection of a compound of interest in a microfluidic system. A second aspect of the present invention relates to the use of the method according to the first aspect for monitoring a biological event. A further aspect of the present invention is directed to a microfluidic system and the use thereof for carrying out the method according to the first aspect.

Abstract: A reaction device for a detection chip and a reaction system are provided. The reaction device includes a chip carrier and an electrical signal control unit. The chip carrier is configured to place and fix the detection chip; and the electrical signal control unit is configured to apply an electrical signal to the detection chip so as to drive a heating electrode of the detection chip.

Abstract: In accordance with one embodiment, a processing device includes a heated internal wall and a rotating rod positioned within an interior space formed by the heated internal wall. The rotating rod may be hollow and act as an internal heat exchanger. The processing device also includes a plurality of baffles spaced apart from one another along the rotating rod and extending away from the rotating rod towards the heated internal wall. The processing device also includes at least one wiper or roller coupled to an edge of at least one of the plurality of baffles or porous, packed basket, coupled to the rotating rod and that contacts the heated internal wall while rotating together with the rotating rod. In another embodiment, a processing device may be used to adsorb reactive gases into a liquid phase while heat is exchanged.

Abstract: Disclosed is a method of producing a test strip for detecting a calcium level in a body fluid test sample obtained from a biological subject such as a human or animal. Also disclosed is a test strip that is useful for determining the concentration of calcium in a human or animal body fluid test sample based on a colorimetric change, and thus provides early detection of osteoporosis by monitoring the calcium excretion rate in an easy and inexpensive manner.

Abstract: A test device and a test method are provided which can prevent a specimen liquid from splattering and leaking and perform an examination simply and safely. The test device includes a culturing unit configured to be capable of culturing a specimen in a sealed state, a test piece configured to be capable of absorbing a specimen liquid in the culturing unit, a separating unit configured to be capable of separating the test piece and the specimen liquid in a non-contact state, an opening unit configured to be capable of opening at least a portion of the separating unit to form a flow path through which the specimen liquid reaches the test piece, and a case configured to integrally seal at least a portion of the test piece on a side closer to the separating unit, at least a portion of the culturing unit, and the separating unit.

Abstract: The invention provides devices and methods for moving charged molecules into and out of tissue samples. This invention is particularly useful for removing endogenous heterogenous particles from tissue samples and for introducing exogenous charged molecules (e.g., antibodies, dyes) into tissue samples.

Abstract: Contamination detection systems, kits, and techniques are described for testing surfaces for the presence of analytes of interest, including hazardous contaminants, while minimizing user exposure to these contaminants. Even trace amounts of contaminants can be detected. A collection device provides a swab that is simple to use, easy to hold and grip, allows the user to swab large areas of a surface, and keeps the user's hands away from the surface being tested. The collection device also includes a test strip, and provides a closed fluid transfer mechanism to transfer the collected fluid from the swab to the test strip while minimizing user exposure to hazardous contaminants in the collected fluid. Contamination detection kits can rapidly collect and detect hazardous drugs, including trace amounts of antineoplastic agents, in healthcare settings at the site of contamination.

Abstract: Temperature measurement is an important part of many potential applications in process industry. Conventional temperature measurement methods require manual intervention for process monitoring and fail to provide accurate and precise measurement of temperature of an enclosed mixed fluid chamber. The present disclosure provides artificial intelligence based temperature measurement in mixed fluid chamber. A plurality of inputs pertaining to the mixed fluid chamber are received to build a fluid based model. The fluid based model is used to generate one or more fluid parameters. The one or more fluid parameters are used along with a ground truth temperature data and the received plurality of inputs for training an artificial intelligence (AI) based model. However, the AI based model is trained with and without knowledge of fluid flow. The trained AI based model is further used to accurately estimate temperature of the mixed fluid chamber for a plurality of test input data.

Abstract: The present invention relates to microfluidic fluidic devices, methods and systems as microfluidic kidney on-chips, e.g. human Proximal Tubule-Kidney-Chip, Glomerulus (Kidney)-Chip, Collecting Duct (Kidney)-Chip. Devices, methods and systems are described for drug testing including drug transport and renal clearance. Further, such devices, methods and systems are used for determining drug-drug interactions and their effect upon renal transporter functions. Importantly, they may be used for pre-clinical and clinical drug development for treating kidney diseases and for personalized medicine.

Abstract: Methods and devices for single cell analysis using fluorescence lifetime imaging microscopy (FLIM) are disclosed. The methods utilize microfluidic devices which use traps to immobilize cells for FLIM analysis. The analysed cells may be sorted before or after imaging and may be plant, animal, or bacterial cells. Analysis of the FLIM data may use a phasor plot and may be used to identify a metabolic pattern of the single cells.

Abstract: Digital microfluidic (DMF) apparatuses, systems, devices and associated fluid manipulation and extraction devices, and methods of using them are presented. The devices may be useful for analysis of clinical, laboratory, chemical, or biological samples. A fluid application and extraction interface device may include a waste reservoir with a fluid trap and a transfer conduit extending through the waste reservoir so that fluid may pass from the transfer conduit into the waste reservoir and be trapped within the waste chamber. A transfer conduit may be configured to double back on itself and to hold a fluid sample. A DMF apparatus may be configured to hold and process large sample volumes.