Patents Examined by Keith C. Farman
  • Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
    Patent number: 5115102
    Abstract: The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depending on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.
    Type: Grant
    Filed: July 21, 1989
    Date of Patent: May 19, 1992
    Assignee: Monsanto Company
    Inventors: Barry L. Haymore, Gary S. Bild, Gwen G. Krivi