Abstract: The invention, in part, includes compositions comprising quadruplet decoding tRNAs and their encoding sequences. The invention also includes assay methods to assess quadruplet decoding as well as methods of preparing quadruplet decoding suppression tRNAs.

Abstract: Described herein are compositions, vectors, cells, methods, and kits that provide cell data recorder systems for recording cell states. The cell data recorder systems allow for the recording of both the presence and duration of one or more stimuli in a programmable, reproducible, and multiplexable manner. These cell data recorder systems employ a nucleic acid programmable DNA binding protein, such as a Cas9 nuclease, or a fusion protein comprising a nucleic acid programmable DNA binding domain and a nucleic acid editing domain to introduce recordable changes in the genome of a cell or in a plasmid within the cell.

Abstract: The present disclosure relates to methods aimed towards non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular region. More particularly, the present disclosure relates to methods of using photoselection to achieve non-invasive targeted genomic and epigenomic sequencing of spatially-defined cellular or subcellular regions, via the use of light-activated probes.

Abstract: The present invention relates to vesicles derived from bacteria of the genus Catenibacterium and a use thereof, and the inventors experimentally confirmed that the vesicles were significantly reduced in samples obtained from patients with a malignant disease such as colon cancer, pancreatic cancer, cholangiocarcinoma, ovarian cancer, bladder cancer or lymphoma, a cardiovascular disease such as myocardial infarction, atrial fibrillation, variant angina or stroke, diabetes, Parkinson's disease, and depression, compared with a normal individual, and that when vesicles isolated from the strain were administered, the secretion of inflammatory mediators caused by pathogenic vesicles, such as E. coli-derived vesicles, was significantly inhibited.

Abstract: The present invention relates to a novel modification of a 5-methylcytosine nucleic acid catalyzed by a Cmd1 enzyme and an application thereof. The present inventors have for the first time discovered Cmd1, a 5mC-modifying enzyme, which can link a glyceryl group to a 5mC methyl carbon of a methylated nucleic acid through a carbon-carbon single bond, and this is a new epigenetic modification.

Abstract: The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-? and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.

Abstract: The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

Abstract: Engineered FnCas9 variants are provided that have an enhanced kinetic activity and a broader PAM recognition. The protein engineering methodology introduced specific mutations that stabilized interaction between Cas9 enzyme and target DNA. The enhanced kinetic activity increases NHEJ-mediated editing, owing to more efficient DSB generation potential than WT FnCas9, and the broadened PAM specificity increases the target range of FnCas9 variants. Thus, the scope and accessibility of CRISPR-Cas9 system targets are widened, along with generating robust and highly specific engineered FnCas9 variants.

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.

Abstract: Disclosed herein are methods, compositions, and kits for engineering proteins using error-prone orthogonal replication (epOrthoRep) and yeast surface display (YSD).

Abstract: Provided herein are gene editing systems and/or compositions comprising RNA guides targeting LDHA for use in genetic editing of the LDHA gene. Also provide herein are methods of using the gene editing system for introducing edits to the LDHA gene and/or for treatment of primary hyperoxaluria (PH), and processes for characterizing the gene editing system.

Abstract: The present disclosure provides a method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, and belongs to the technical field of microorganisms. The method includes: mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid, and subjecting a resulting mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella. The method provided by the present disclosure can successfully knock out the NMT gene from Eimeria tenella, which lays a foundation for studying the function of the Eimeria tenella gene and developing a vaccine therefor.

Abstract: This disclosure relates mRNA therapy for the treatment of ornithine transcarbamylase deficiency (OTCD). mRNAs for use in the invention, when administered in vivo, encode human ornithine transcarbamylase (OTC), isoforms thereof, functional fragments thereof, and fusion proteins comprising OTC. mRNAs of the invention are preferably encapsulated in lipid nanoparticles (LNPs) to effect efficient delivery to cells and/or tissues in subjects, when administered thereto. mRNA therapies of the invention increase and/or restore deficient levels of OTC expression and/or activity in subjects. mRNA therapies of the invention further decrease levels of toxic ammonia associated with deficient OTC activity in subjects.

Abstract: A motor assembly is provided for use with projectiles, such as munitions, having relatively low length to diameter ratios. The motor assembly has an aerospike nozzle and a casing disposed about the aerospike nozzle, where interior aerospike volume contains propellant and where walls of both the cowl of the casing and of the aerospike nozzle jointly define a combustion chamber.

Abstract: A cylindrically-shaped hybrid rocket engine solid fuel grain defines an axial combustion port. A fuel grain material comprises a compounded blend of thermoplastic fuel and aluminum. The fuel grain comprises fused stack layers, each layer comprising a plurality of fused abutting concentric beaded structures arrayed to define the combustion port; the port exhibits a rifling pattern or rifling inducing geometry along the port wall. When an oxidizer is introduced into the combustion port combustion occurs along the exposed port wall. Each beaded structure defines a geometry that increases the combustion surface area while inducing a vortex flow of oxidizer and fuel gas. As each layer ablates, an abutting layer exhibiting a similar geometry, is revealed, undergoes a gas phase change, and ablates. This process repeats and persists until oxidizer flow is terminated or the fuel grain material is exhausted. The fuel grain may be manufactured by an additive manufacturing process.

Abstract: In one aspect, the present subject matter is directed to a gas turbine sealing assembly that includes a first static gas turbine wall and a second static gas turbine wall. A seal is disposed between the first static gas turbine wall and the second static gas turbine wall. The seal includes a first seal layer defining a first seal layer aperture extending therethrough. A second seal layer defines an elongated slot extending therethrough. The elongated slot includes a first end and a second end. A third seal layer defines a third seal layer aperture extending therethrough. The second seal layer is positioned between the first seal layer and the third seal layer such that the first seal layer aperture is in fluid communication with the first end and the third seal layer aperture is in fluid communication with the second end.

Abstract: Shrouds and shroud assemblies for gas turbine engines are provided. A shroud includes a shroud body. The shroud body includes a forward surface, a rear surface axially spaced from the forward surface, an inner surface extending between the forward surface and the rear surface, and an outer surface extending between the forward surface and the rear surface and radially spaced from the inner surface. The shroud further includes a flange extending from the shroud body, and a bore hole defined in the flange. The bore hole extends generally circumferentially through the flange between a first opening and a second opening.

Abstract: An arrangement (10) for delivering gases from combustors (15) to a first row of blades. The arrangement (10) includes at least an upstream flow path (60) including an aft first side wall (64) and a downstream flow path (62) including a forward second side wall (66). A convergence junction trailing edge (40) is defined at a downstream terminal edge (41) of the first side wall (64), and the second side wall (66) converges toward the first side wall (64) in the direction of the convergence junction trailing edge (40). An impingement sheet structure (78) is located between and provides impingement cooling air to the first and second side walls (64, 66). Openings (88) provide a cooling air passage between the first and second side walls (64, 66) and provide a flow of post impingement air into the gas path at the convergence junction trailing edge (40).

Abstract: An adjustable size inlet duct system for a ducted fan propulsion system may change its inlet profile to enhance thrust levels in low-speed and hovering flight conditions and may change its inlet profile to reduce drag to permit faster flight speeds at higher operating speeds. The adjustable size inlet duct system may include duct petals and associated actuators. During slow speed flight, the petals may expand to generate a rounded large inlet area bellmouth profile. At higher flight speeds, the petals may contract to reduce the profile drag of the duct in forward flight. An optional exterior bypass duct may be included to provide additional thrust in excess of the thrust provided by the ducted fan. An optional annular electric motor housed in the exterior bypass duct may be included to generate still more excess thrust.

Abstract: A micromixer system includes a casing having a first side wall and a second side wall. Further, the micromixer system includes a plurality of pipes spaced apart from each other and disposed within the casing. Each pipe includes an inlet and an outlet formed in the first and second side walls respectively. The micromixer system includes a first plenum having a first inlet formed in the casing. The first plenum is disposed around a first portion of the plurality of pipes and fluidically coupled to the plurality of pipes. The micromixer system includes a second plenum having a second inlet formed in the casing and disposed around a second portion of the plurality of pipes. Further, the micromixer system includes a plurality of openings formed in the second side wall, surrounding the outlets of at least some pipes of the plurality of pipes, and fluidically coupled to the second plenum.