Patents Examined by Laura Schuberg
  • Patent number: 10358629
    Abstract: Provided are methods for producing progenitor/precursor cells from a population of initiating cells (ICP) that have a density of less than 1.072 g/ml and at least 25% of which are CD31Bright by in vitro stimulating the ICP with different factors.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: July 23, 2019
    Assignee: Kwalata Trading Limited
    Inventors: Yael Porat, Valentin Fulga, Svetlana Porozov, Adina Belleli
  • Patent number: 10307444
    Abstract: Methods are provided for the production of photoreceptor cells and photoreceptor progenitor cells from pluripotent stem cells. Additionally provided are compositions of photoreceptor cells and photoreceptor cells, as well as methods for the therapeutic use thereof. Exemplary methods may produce substantially pure cultures of photoreceptor cells and/or photoreceptor cells.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: June 4, 2019
    Assignee: Astellas Institute for Regenerative Medicine
    Inventors: Robert P. Lanza, Shi-Jiang Lu, Wei Wang
  • Patent number: 10265345
    Abstract: The present invention provides a method for treatment of acute cerebrovascular diseases, and also provides the use of the extracts from rabbit skin inflamed by vaccinia virus in the manufacture of a medicament for treatment of acute cerebrovascular diseases.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: April 23, 2019
    Assignee: Vanworld Pharmaceutical (Rugao) Co., Ltd.
    Inventor: Mansang Lau
  • Patent number: 10260044
    Abstract: The present disclosure relates to a method of expanding myeloid progenitor cells by culturing an initial population of cells in a medium comprising a mixture of cytokines and growth factors that promote growth and expansion of the myeloid progenitor cells. The expanded cell population provides a source of cells as therapeutic treatments for neutropenia and/or thrombocytopenia arising in patients subjected to myeloablative therapy and hematopoietic stem cell transplantation.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: April 16, 2019
    Inventors: Timothy C. Fong, Adrianus Geertrudis Wilhelmus Domen, Julie Lynne Christensen
  • Patent number: 10172793
    Abstract: A probiotic tablet comprising a probiotic micro-organism and other nutritionally active ingredients in two zones, a first zone comprising said probiotic micro-organism and prcicrably selenium, and a second zone comprising at least one said other active ingredient such as iron, other minerals and vitamin B6 kept separated from the probiotic micro-organism of said first zone, the water activity in said probiotic micro-organism containing first zone being no greater than 0.2 and the water content of said tablet being as much as 3% by weight. Good viability of the micro-organisms is obtained despite the relatively high overall moisture content.
    Type: Grant
    Filed: December 21, 2004
    Date of Patent: January 8, 2019
    Assignee: Ferrosan A.S.
    Inventors: Kristian Lund Henriksen, Helene Mathilda Mortensen, Marianne Winning
  • Patent number: 10131880
    Abstract: Methods for the efficient isolation and use of pluripotent adipose-derived stem cells (PASCs) are provided. In certain embodiments the methods involve providing an adipose tissue sample from which the stromal vascular fraction is co-cultured with the adipocyte fraction. PASCs can be isolated with a high degree of purification without requiring an additional cell enrichment process (e.g. cell sorting). PASCs and their conditioned media can be used for tissue regeneration within hours of harvesting the adipose tissue, and without requiring cell expansion. PASCs can grow as floating individual cells, as clusters of cells, or attached to surface(s) of the culture vessel. PASCs do not produce teratomas in vivo, nor do they induce immunorejection upon transplantation, and they achieve a high efficiency in grafting. The cells and compositions can be used for cell therapy and to screen new drugs.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: November 20, 2018
    Inventor: Gregorio Chazenbalk
  • Patent number: 10046012
    Abstract: The invention generally relates to postnatal periodontal ligament stem cells and methods for their use. More specifically, the invention relates in one aspect to postnatal periodontal ligament multipotent stem cells, use of the cells to generate periodontium, differentiation of the cells and methods of tissue cryopreservation.
    Type: Grant
    Filed: November 9, 2015
    Date of Patent: August 14, 2018
    Assignee: The United States of America as represented by the Secretary, Department of Health and Human Services
    Inventors: Songtao Shi, Byoung-Moo Seo, Masako Miura
  • Patent number: 9951312
    Abstract: Chondrocytes are prepared from perichondrocytes. The present invention provides a cell derived from a perichondrial tissue, the cell being capable of differentiating into a chondrocyte. The present invention also provides a method of preparing the above-described cell and a composition comprising the same. A method of preparing a chondrocyte and a medium for use in the method are also provided.
    Type: Grant
    Filed: January 23, 2008
    Date of Patent: April 24, 2018
    Assignee: Yokohama City University
    Inventors: Hideki Taniguchi, Shinji Kobayashi
  • Patent number: 9938498
    Abstract: The present invention relates to a method for inducing and expanding natural killer cells derived from peripheral blood mononuclear cells, which comprises co-culturing, as feeder cells, irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells in the presence of cytokines, along with peripheral blood mononuclear cells. According to the present invention, a large quantity of natural killer cells can be induced and proliferated from a small quantity of peripheral blood mononuclear cells even without the use of high-cost equipment or various kinds of expensive cytokines, thereby making it possible to significantly improve the efficiency and efficacy of the prevention and treatment of cancer using the natural killer cells.
    Type: Grant
    Filed: May 7, 2013
    Date of Patent: April 10, 2018
    Assignee: NKMAX Co., Ltd.
    Inventors: Kyung Mi Lee, Seon Ah Lim, Cassian Yee
  • Patent number: 9879226
    Abstract: Disclosed is a basic culture medium for mesenchymal stem cells, and a cell therapeutic agent cultured and differentiated using same. The basic culture medium reduces the time taken from collection to mass culturing by increasing the proliferation rate of undifferentiated mesenchymal stem cells derived from an adult tissue such as human marrow and adipose tissue, and also is capable of various differentiations into treating agents for bone-forming cells, for cartilage cells, or for fat cells.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: January 30, 2018
    Inventors: Dong-Sam Suh, Jun Keun Lee, Dong Il Chang, Min Jung Choi, Jang Hoon Kim, Ga Ram Kim, Cheong Ho Chang
  • Patent number: 9868939
    Abstract: The present invention relates generally to methods and compositions useful for therapeutic vascular tissue engineering. In particular, the present invention provides methods for generating substantially pure populations of vasculogenic cells from human mesenchymal progenitors, and methods and compositions for clinical applications in the field of regenerative medicine.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: January 16, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Igor Slukvin, Akhilesh Kumar
  • Patent number: 9822331
    Abstract: The method comprises an automated removal of cells and/or cell colonies from a cell culture whilst executing a first detection step for selecting cells and/or cell colonies with reference to corporeal and/or physical parameters and detecting position data and storing the detected position data of the selected cells and/or cell colonies in a position database. In order to be able to select special cells and/or cell colonies having special properties from the detected cells and/or cell colonies, at least one second detection step for detecting at least one further parameter of the cells and/or cell colonies is then executed, comparative data from the data of the first and second detection step are created, cells and/or cell colonies are selected with reference to the comparative data and the position data from the position database are transferred to a harvesting unit.
    Type: Grant
    Filed: September 20, 2007
    Date of Patent: November 21, 2017
    Inventors: Rafael Backhaus, Jens Eberhardt, Marco Meinhard, Gerd Bornmann
  • Patent number: 9783777
    Abstract: The method of making a three-dimensional, leaf-based scaffold for three-dimensional cell cultures includes washing a quantity of Ficus religiosa leaves, then treating the washed Ficus religiosa leaves in a sodium hydroxide solution to obtain alkali-treated Ficus religiosa leaves. The alkali-treated Ficus religiosa leaves are washed, and then superficial tissue is removed from the alkali-treated Ficus religiosa leaves to obtain Ficus religiosa leaf skeletons. The Ficus religiosa leaf skeletons are dried and then consecutively immersed in distilled water, a phosphate buffer saline solution, and plain Dulbecco's modified Eagle's medium (DMEM) to form the three-dimensional scaffolds for three-dimensional cell cultures. Each three-dimensional scaffold can be used for growing three-dimensional cell cultures, such as human mesenchymal stem cell cultures.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: October 10, 2017
    Inventors: Periasamy Vaiyapuri Subbarayan, Ali Abdullah Alshatwi, Jegan Athinarayanan
  • Patent number: 9771558
    Abstract: The invention relates in certain embodiments to a method for generating dendritic cells by employing temperatures below 37° C. during the development of progenitor cells and immature dendritic cells. In some embodiments the invention relates to populations of dendritic cells and its use.
    Type: Grant
    Filed: December 7, 2006
    Date of Patent: September 26, 2017
    Inventors: Alexei Kirkin, Karine Djandjougazian, Jesper Zeuthen
  • Patent number: 9765297
    Abstract: The present invention relates to culture systems comprising differentiated stem cells, that may be used for identifying agents useful in treating degenerative nervous system disorders and are suitable for high-throughput screening applications. It is based, at least in part, on the discovery that co-cultures of (i) astrocytes expressing a mutated SODI gene and (ii) stem-cell derived motor neurons manifested cell death via a Bax-dependent mechanism, and modeled motor neuron death in amyotrophic lateral sclerosis.
    Type: Grant
    Filed: April 10, 2008
    Date of Patent: September 19, 2017
    Inventors: Serge Przedborski, Hynek Wichterle, Makiko Nagai, Thomas M. Jessell
  • Patent number: 9759640
    Abstract: An embodiment of the claimed invention is directed to a method that greatly streamlines and reduces costs for tissue preparation, preservation, long-term storage and sample retrieval for molecular analysis using a method based on dried blood spot (DBS) technology. In this method, a small needle punch sample of freshly excised tissue will be homogenized in stabilizing reagent and inserted into a device containing absorbent material and drying agent. This device is suitable for long-term sample storage at ambient temperature and allows for easy removal of sections for biomarker analysis.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 12, 2017
    Assignee: Spot on Sciences, Inc.
    Inventors: James Hill, Jeanette Hill
  • Patent number: 9752124
    Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: September 5, 2017
    Assignee: Koninklijke Nederlandse Akademie van Wetenschappen
    Inventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
  • Patent number: 9708582
    Abstract: The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: July 18, 2017
    Assignee: Stemcell Technologies Inc.
    Inventors: Alexandra A. Blak, Sharon A. Louis, Michael John Riedel
  • Patent number: 9611453
    Abstract: The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.
    Type: Grant
    Filed: March 10, 2008
    Date of Patent: April 4, 2017
    Assignee: The Regents of the University of California
    Inventors: Sangeeta N. Bhatia, Elliot Hui
  • Patent number: 9581596
    Abstract: A method for detecting HIV infection in a mammal is disclosed. The method contains the steps of isolating exosomes from a urine sample of a mammal and detecting the presence of HIV-specific biomarker in said isolated exosomes. A method for diagnosing a mammal with an HIV-associated disease, in particular, HIV-associated nephropathy is also disclosed.
    Type: Grant
    Filed: January 11, 2012
    Date of Patent: February 28, 2017
    Inventors: Gale W. Newman, Mike Powell, Akins Doherty, Chamberlain Obialo