Patents Examined by Leon B. Lankford, Jr.
  • Patent number: 10092679
    Abstract: Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.
    Type: Grant
    Filed: October 20, 2014
    Date of Patent: October 9, 2018
    Assignee: Wake Forest University Health Sciences
    Inventors: Hyunhee Ahn, Young Min Ju, Anthony Atala, Sang Jin Lee
  • Patent number: 10085935
    Abstract: Provided herein is a bee venom composition with effects of protecting and beautifying lips, comprising cosmetic matrix components for use on lips and bee venom. The bee venom is prepared by using the following method: crude bee venom is dissolved with water, before ultrafiltrated using an ultrafiltration membrane with the molecular weight cutoff 10000 Da, and then the resulting filtrate is freeze-dried to obtain the bee venom.
    Type: Grant
    Filed: January 24, 2013
    Date of Patent: October 2, 2018
    Inventors: Hua Chen, Huili Sun, Si Zhang, Jianyu Pan, Bingna Cai, Xiaoyu Sun
  • Patent number: 10080730
    Abstract: Provided are methods for treating or preventing vasculopathy in a subject in need thereof, comprising administering to the subject a prostacyclin and a mesenchymal stem cell (MSC) or a MSC-conditioned culture medium or administering to the subject a MSC or a MSC-conditioned culture medium that has treated with prostacyclin. Pharmaceutical compositions suitable for such treatments are also provided.
    Type: Grant
    Filed: January 8, 2014
    Date of Patent: September 25, 2018
    Assignee: United Therapeutics Corporation
    Inventors: Roger Jeffs, Thomas Petersen, Roger M. Ilagan, Michael Wade
  • Patent number: 10082499
    Abstract: The present invention relates to a method for preparation of labeled metabolic products, comprising the steps (a) providing a biological sample in vitro, (b) contacting the biological sample with a labeling buffer comprising a labeled substrate, wherein the substrate comprises at least one carbon atom and represents an educt or intermediate of a metabolic process of the biological sample and wherein the label is a stable isotope, (c) washing the biological sample using a wash-buffer, wherein said wash-buffer comprises a carbon resource such that the biological sample is precluded from carbon deficiency during said washing, wherein said carbon resource comprises the substrate according to step b), wherein said substrate in the wash-buffer may be labeled or unlabeled, (d) quenching the biological sample such that metabolic processes within the biological sample are slowed down or stopped and (e) extracting the labeled metabolic product from the biological sample.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: September 25, 2018
    Inventors: Stefan Kempa, Matthias Pietzke, Christin Zasada
  • Patent number: 10076545
    Abstract: The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.
    Type: Grant
    Filed: April 17, 2013
    Date of Patent: September 18, 2018
    Assignee: EnGeneIC Molecular Delivery Pty. Ltd.
    Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
  • Patent number: 10039789
    Abstract: The present invention relates to compositions and methods for cell transplantation. In particular, the present invention provides a composition comprising procoagulant cells and at least one antithrombin activator, preferably unfractionated heparin, as well as at least one thrombin inhibitor, preferably bivalirudin.
    Type: Grant
    Filed: October 6, 2016
    Date of Patent: August 7, 2018
    Inventors: Xavier Stephenne, Etienne Sokal, Mustapha Najimi, Stéphane Eeckhoudt, Cédric Hermans
  • Patent number: 7883874
    Abstract: Novel strains of Bifidobacterium hifidum capable of producing a novel galactosidase enzyme activity that converts lactose to a novel mixture of galactooligosaccharides. The mixture of oligosaccharidcs may be incorporated into numerous food products or animal feeds for improving gut health by promoting the growth of bifidobacteria in the gut, and repressing the growth of the pathogenic microflora.
    Type: Grant
    Filed: June 30, 2004
    Date of Patent: February 8, 2011
    Assignee: Clasado Inc.
    Inventors: Glenn Gibson, Jacek Witold Slupinski, Georgios Tzortzis, Anthony Graham Wynne
  • Patent number: 7879076
    Abstract: Enhancing the effectiveness of therapeutic ionizing radiation and other treatments of disease in which cells are to be destroyed or modified, by subjecting cells in need thereof to low-dose radiation to increase the sensitivity of the cells to subsequent subjection with a lethal dose of high dose radiation (HDR), a chemotherapeutic agent, or other type of therapeutic treatment.
    Type: Grant
    Filed: August 28, 2006
    Date of Patent: February 1, 2011
    Assignee: The Catholic University of America
    Inventors: Theodore Aaron Litovitz, Andrea Lynn Cohen, Patrick Mehl
  • Patent number: 7727755
    Abstract: An enzyme isolated from an extremophilic microbe, and a method for utilizing same is described, and wherein the enzyme displays optimum enzymatic activity at a temperature of greater than about 80° C., and a pH of less than about 2, and further may be useful in methodology including pretreatment of a biomass so as to facilitate the production of an end product.
    Type: Grant
    Filed: November 2, 2005
    Date of Patent: June 1, 2010
    Assignee: Battelle Energy Alliance, LLC
    Inventors: Vicki S. Thompson, David N. Thompson, Kastli D. Schaller, William A. Apel
  • Patent number: 7588940
    Abstract: An apparatus and method for manipulating, effecting interaction of, photochemically transforming and/or sorting small dielectric particles or other materials. The apparatus and method involves use of one or more diffractive optical elements which each receive a laser beam and form a plurality of laser beams. These laser beams are operated on by a telescope lens system and then an objective lens element to create an array of optical traps for manipulating, effecting interaction of, photochemically transforming and/or sorting small dielectric particles or other materials.
    Type: Grant
    Filed: January 6, 2005
    Date of Patent: September 15, 2009
    Assignee: University of Chicago
    Inventors: David G. Grier, Eric R. Dufresne
  • Patent number: 7566470
    Abstract: The invention generally relates to a method for fractionating plant material into valuable components including beta-glucan. The method uses an organic solvent and water instead of water alone, acidified water and/or aqueous alkali as a solvent for the slurrying of a grain flour. In addition to concentrating beta-glucan, other product fractions produced by the method include starch concentrate, and organic solvent solubles. If enzyme treatments are used, other product fractions include dextrin, protein hydrolysates and organic solvent solubles. The process is particularly effective in concentrating beta-glucans in a state close to its native form from the endosperm of barley and oat grains.
    Type: Grant
    Filed: September 26, 2001
    Date of Patent: July 28, 2009
    Assignee: Governors of the University of Alberta, The
    Inventors: Thavaratnam Vasanthan, Feral Temelli
  • Patent number: 7566550
    Abstract: The present invention relates to a package of screening methods for developing drugs against pathogenic microbes having two-component system of DevR-DevS and/or DevR-Rv2027c and its homologues, said method comprising steps of over-expressing DevR, DevS, and Rv2027c and their single domain derivatives including mutant variant proteins, autophosphorylating DevS, and Rv2027c proteins and thereafter, phosphotransfering to DevR and its derivatives in SDS-PAGE or High-throughput format in the presence of a test compound, and determining the drug-potential of the test compound, wherein the potential of the drug is inversely proportional to (i) the degree of autophosphorylation of DevS and Rv2027c, (ii). the degree of phosphotransfer-based dephosphorylation of DevR and/its single domain derivative, and (iii). the degree of dephosphorylation of phosphorylated species of DevS and Rv2027c and/their single domain derivatives, and a method of treatment, and a composition thereof.
    Type: Grant
    Filed: October 16, 2003
    Date of Patent: July 28, 2009
    Assignee: Council of Scientific & Industrial Research
    Inventors: Jaya Sivaswami Tyagi, Deepak Kumar Saini
  • Patent number: 7537885
    Abstract: The present invention is directed to methods of preserving mammalian heart, ex vivo. Nanoparticle compositions for carrying out the invention are also disclosed.
    Type: Grant
    Filed: March 22, 2007
    Date of Patent: May 26, 2009
    Assignee: Lifeblood Medical Inc.
    Inventors: Joseph Fischer, Jan Baker, Robert G. L. Shorr
  • Patent number: 7491340
    Abstract: A method for treating a living organism to increase nutrient ingestion. The step of the method are first treating a fluid stream for use by at least one living organism, in a surface tension modification generator (STMG) at a first pressure with a frequency minimize hydrogen bonding of the fluid forming a reduced surface tension stream. The next step is flowing the reduced surface tension stream through a port reducing the first pressure to a second pressure and adding oxygen. After oxygen is added, then flow the reduced surface tension stream with oxygen at the second pressure into an inner chamber. The next step is flowing the reduced surface tension stream and oxygen from the inner chamber through a plurality of funnels disposed in a wall of the inner chamber creating a turbid zone in an outer chamber, and wherein nano-sized oxygen particles are dissolved into the stream.
    Type: Grant
    Filed: May 27, 2005
    Date of Patent: February 17, 2009
    Assignee: Innovative Industries, Inc.
    Inventors: James Richard Von Krosigk, Larry John Dove
  • Patent number: 7449171
    Abstract: Methods of determining rate of biosynthesis or breakdown of biological molecules from metabolic derivatives and catabolic products are disclosed herein. In particular, methods of measuring the rates of biosynthesis and breakdown of biological molecules inaccessible or not easily accessible to direct sampling by sampling metabolic derivatives and catabolic products in accessible biological samples are disclosed herein.
    Type: Grant
    Filed: February 12, 2003
    Date of Patent: November 11, 2008
    Assignee: The Regents of the University of California
    Inventor: Marc K. Hellerstein
  • Patent number: 7439036
    Abstract: To provide an enzymatic resolution process for efficiently producing an optically active N-protected-octahydro-1H-indole-2-carboxylic acid denoted by the formula (2): by using an enzyme capable of asymmetrically hydrolyzing the —CO2R1 group in the formula (1) wherein R1 indicates an alkyl group having a carbon number of 1 to 4, R2 indicates a protecting group of the imino group, and the carbon atoms marked with asterisks (*) indicate asymmetrical carbon atoms.
    Type: Grant
    Filed: October 28, 2004
    Date of Patent: October 21, 2008
    Assignee: Sumitomo Chemical Company, Limited
    Inventor: Norihiko Hirata
  • Patent number: 7427397
    Abstract: The present invention relates to probiotic Propionibacterium strains and their use in the preparation of probiotic supplements and foods. The invention relates to the provision of Vitamin B12, propionic acid, folacin and bacteriocins by probiotic strains, stimulation of bifidobacteria growth, production of favourable effects on the lipid metabolism and on the immune system of hosts through immunostimulation, immunomodulation or use of a probiotic strain as an adjuvant, reduction of homocysteine and ? glucuronidase and the prevention, treatment or amelioration of conditions associated with a need for these activities. The probiotic bacteria of the invention can be used in humans or other animals. In at least some applications, the bacteria can be used dead and parts rather than whole cells may be used. The present invention also relates to the preparation of vaccines for use in protecting patients from infectious diseases, in particular tuberculosis.
    Type: Grant
    Filed: December 21, 2004
    Date of Patent: September 23, 2008
    Assignee: University of Newcastle Research Associates (TUNRA) Ltd.
    Inventors: Michelle Catherine Adams, Yang Huang
  • Patent number: 7422893
    Abstract: An apparatus for growing cells in a 2-D cell cassette having a matrix for cell growth under pressure comprising two side assemblies which include a frame with a rigid support that fits over the 2-D cell cassette, with said assemblies positioned on either side of the cassette over the solid support sides, and a means to secure the sides of the cassette. The invention also provides different uses of this apparatus.
    Type: Grant
    Filed: March 3, 2003
    Date of Patent: September 9, 2008
    Assignee: Memorial Sloan-Kettering Cancer Center
    Inventors: Gene R. Diresta, John H. Healey, Robert Schwar
  • Patent number: 7402322
    Abstract: The invention relates to the field of immunology. Specifically, the invention relates to the field of immune-mediated disorders such as allergies, auto-immune disease, transplantation-related disease or inflammatory disease. The invention provides for an immunoregulator (IR), use of an IR in preparing a pharmaceutical composition for treating an immune-mediated disorder and a method for treating an immune-mediated disorder.
    Type: Grant
    Filed: October 3, 2003
    Date of Patent: July 22, 2008
    Assignee: Biotempt B.V.
    Inventors: Nisar Ahmed Khan, Hubertus Franciscus Josef Savelkoul, Robbert Benner
  • Patent number: 7402432
    Abstract: The present invention is directed to an in vitro method for producing T lymphocytes. The method involves culturing bone marrow cells on a matrix seeded with keratinocytes and fibroblasts.
    Type: Grant
    Filed: December 7, 2004
    Date of Patent: July 22, 2008
    Assignee: The Brigham and Women's Hospital, Inc.
    Inventors: Rachael A. Clark, Thomas Kupper