Abstract: The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis.

Abstract: The present invention relates to unique strategies and constructs for producing a nucleic acid product that downregulates or prevents expression of a desired target polynucleotide.

Abstract: Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants.

Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of plants generated or maintained by the methods, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, and methods for repressing transmission of transgenes.

Abstract: Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants with increased nitrogen use efficiency. The present invention further relates to nucleotide sequences and the use of those nucleotide sequences in the genetic-engineering of plants to display enhanced nitrogen assimilatory and utilization capacities, grow larger, more efficiently or rapidly, and/or have enriched nitrogen contents in vegetative and/or reproductive plant parts and/or increased biomass. More particularly, this invention relates to producing transgenic plants engineered to have altered expression of key components in the nitrogen assimilation and utilization pathways. The engineered plants may be productively cultivated under conditions of low nitrogen fertilizer input or in nitrogen poor soils.

Abstract: A system for expression of a heterologous polypeptide in a transgenic host cell is disclosed. The system is based upon a transgene comprising a eukaryotic promoter operably linked to a DNA sequence comprising, in the 5? to 3? direction, a DNA sequence complementary to a sequence encoding a heterologous polypeptide, a DNA sequence complementary to an internal ribosome entry site, and a DNA sequence corresponding to a 3? untranslated region of a positive strand single-stranded RNA virus. Following introduction of a stimulus, the host cell synthesizes an RNA molecule complementary to a recombinant RNA encoded by the transgene. The stimulus can be a positive strand single-stranded RNA virus or a nucleic acid thereof. Because the complement of the recombinant RNA comprises an internal ribosome entry site and a sequence encoding a heterologous polypeptide, the host cell can synthesize the heterologous polypeptide.

Abstract: The present invention provides a transgenic maize plant having in its genome transgenic DNA including sequence for zein reduction and sequence for lysine biosynthesis, whereby expression of the transgenic DNA results in a synergistically increased lysine content of seed of the transgenic maize plant. The invention further provides a method for providing maize seed with synergistically increased lysine content.

Abstract: The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.

Abstract: The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.

Abstract: The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

Abstract: The invention provides methods for decreasing lignin content and improving lignin profiles. Also provided are the plants prepared by the methods of the invention. Such plants may exhibit improved digestibility relative to prior plants.

Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

Abstract: A novel plant virus vector and a method of gene expression into plants are provided, and the present invention relates to a plant virus vector characterized in that a part of the sequence of the 2b region of the RNA2 molecule of the Cucumber mosaic virus (CMV) is deleted; the aforementioned plant virus vector characterized in that the region from the StuI site to the stop codon of the 2b ORF of pCY2, which is an infectious clone of CMV-Y RNA2, is deleted; and a method of gene expression in plants characterized in that a foreign gene is introduced so as to be stably expressed in a plant using the genetic recombinant. This vector is useful as a plant RNA vector for causing stable expression of foreign genes.

Abstract: The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The invention also provides methods for expressing a heterologous polypeptide in a plant such as soybean. The invention additionally provides methods for virus-induced gene silencing, particularly in a soybean plant, which can be used to determine the function of a gene of interest.

Abstract: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

Abstract: The invention provides methods for transforming monocotyledonous plants with Agrobacterium, for example, using stolons as a target tissue. The invention allows creation of transgenic plants without the need for callus as a target tissue for transformation, thus providing a rapid method for the production of transgenic plants. The ability to directly regenerate transgenic plants from stolons significantly reduces the time, labor and other complications required to produce transgenic plants. Plants produced by such methods are also provided by the invention.

Abstract: The present invention relates to a novel method for increasing the resistance, of a plant, in particular of a Solanaceae, preferably of potato and tomato, to plant pathogens of the phylum Oomyceta comprising increasing the activity of the polypeptid of the present invention. The invention further relates to polynucleotides and vectors comprising these polynucleotides. The invention furthermore relates to corresponding vectors, cells, transgenic plants and transgenic propagation material derived from them, methods to produce them and to their use for the production of foodstuffs, feeding stuffs, seed, pharmaceuticals or fine chemicals.

Abstract: In one aspect, the invention provides novel tetraploid Brassica plants having no more than two expressible FAD2 coding sequences, capable of producing canola quality oils. Other aspects of the invention provide new variants of the FAD2 enzyme, comprising BjFAD2-b and BjFAD2-a, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include nucleic acid sequences upstream from the BjFAD2-b or BjFAD2-a ORFs. Other aspects of the invention include transgenic plants and plant pads. Vectors capable of transforming plant cells are provided, comprising the nucleic acids of the invention, including FAD2 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products.

Abstract: The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

Abstract: The invention relates to a method to inactivate plant genes. In one embodiment, the method comprises the steps of providing a viral vector comprising a cDNA sequence reverse transcribed from RNA of Cymbidium mosaic virus; constructing a recombinant plasmid containing a homologous gene fragment of the plant and the viral vector; preparing transcripts of the plasmid through in vitro transcription; infecting the plant with the transcripts; and forming siRNA from the transcripts to inactivate the homologous gene.