Patents Examined by Lianko Garyu
  • Patent number: 8637472
    Abstract: The invention relates peptide entry inhibitors and methods of determining such inhibitors that are bindable to regions of viruses having class II E proteins, such as the dengue virus E protein, as candidates for in vivo anti-viral compounds.
    Type: Grant
    Filed: July 11, 2008
    Date of Patent: January 28, 2014
    Assignees: University of Washington Through Its Center for Commercialization, Florida Gulf Coast University
    Inventors: Scott F. Michael, Sharon Isern, Joshua Costin, Vaikuntanath Samudrala, Ekachai Jenwitheesuk
  • Patent number: 8623811
    Abstract: The present invention provides antimicrobial peptides that exhibit a broad range of antimicrobial activity to gram positive and gram negative bacteria, as well as fungi, mold, and virus. The antimicrobial peptide of the invention is a cationic peptide, and may contain an HIV-TAT or reverse HIV-TAT sequence, or derivative thereof. The present invention further provides antimicrobial compositions containing the cationic peptide. Such compositions are especially useful for topical application to the skin, hair, nail, vagina, urethra, ear, oral cavity, nasal passage, respiratory system, opthalmic region, and various mucosal regions. The compositions of the present invention improve the condition and/or appearance of the treated region, and are suited for long-term and/or routine use to, for example, prevent or prevent the recurrence of microbial infection. The present invention further provides kits for use in improving the condition or appearance of skin, nail, or treated area.
    Type: Grant
    Filed: July 28, 2008
    Date of Patent: January 7, 2014
    Assignee: Revance Therapeutics, Inc.
    Inventors: Hongran F. Stone, Jacob M. Waugh
  • Patent number: 8580548
    Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.
    Type: Grant
    Filed: February 29, 2012
    Date of Patent: November 12, 2013
    Assignee: Thermo Fisher Scientific Baltics, UAB
    Inventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
  • Patent number: 8563477
    Abstract: A composition including (a) a solid support having a surface; (b) a first plurality of nucleic acids immobilized on the surface, wherein the nucleic acids in the first plurality each include the P7 primer sequence (5?-CAAGCAGAAGACGGCATACGA-3?; SEQ ID NO: 3); and (c) a second plurality of nucleic acids immobilized on the surface, wherein the nucleic acids in the second plurality each include the P5 primer sequence (5?-AATGATACGGCGACCACCGA-3?; SEQ ID NO: 4).
    Type: Grant
    Filed: July 13, 2012
    Date of Patent: October 22, 2013
    Assignee: Illumina Cambridge Limited
    Inventors: Mark Edward Brennan Smith, Andrea Sabot, Isabelle Marie Julia Rasalonjatovo, Jean-Ernest Sohna Sohna, Adrian Martin Horgan, Harold Philip Swerdlow
  • Patent number: 8530431
    Abstract: Disclosed herein are methods of treating cancer by administering a modified Human Immunodeficiency Virus (HIV) trans-activator of transcription (Tat) polypeptide with increased immunostimulatory properties relative to the non-modified Tat polypeptide.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: September 10, 2013
    Assignee: PIN Pharma, Inc.
    Inventor: David I. Cohen
  • Patent number: 8465972
    Abstract: A cell culture article including a substrate having nanoparticles on the substrate surface, the nanoparticle including: a polymer of formula (I) where (x), (y), (z), R, R?, R?, S, W, and X, are as defined herein. Methods for making the cell culture article or cell culture article and methods for performing an assay of a ligand with the article are also disclosed.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: June 18, 2013
    Assignee: Corning Incorporated
    Inventors: Wendy A. Baker, Bertrand De Lambert, David Henry, Odessa N. Petzold
  • Patent number: 8466258
    Abstract: The present invention relates to diagnostic imaging and in particular to the diagnostic imaging of fibrosis. More particularly, the present invention provides a polypeptides, cyclic polypeptides and pharmaceutical compositions suitable for the non-invasive visualization of fibrosis. The polypeptide of the invention may comprise an amino acid sequence consisting of: X1-X2-M-H-G-L-X7-L-X9-X10-D-E (SEQ ID NO: 1) wherein amino acid X1 is R, F or P; amino acid X2 is F or V; amino acid X7 is Q, H or L; amino acid X9 is W or G and amino acid X10 is A or D.
    Type: Grant
    Filed: December 11, 2008
    Date of Patent: June 18, 2013
    Assignees: INSERM (Institut National de la Santé et de la Recherche Médicale), Universite Paris Diderot—Paris 7
    Inventors: Martine Jandrot-Perrus, Julien Muzard, Philippe Billiald, Dominique le Guludec, Laure Sarda, Alain Meulemans
  • Patent number: 8450468
    Abstract: The present invention relates to novel nucleic acid ligands or aptamers that bind to and inhibit the activation of the ?-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subtype of ionotropic glutamate receptors. Also disclosed is a novel combination of technologies, i.e., SELEX and laser pulse photolysis for the selection and screening of aptamers that inhibit receptor function and are useful therefore, in the treatment of diseases associated with excessive activation of ionotropic glutamate receptors.
    Type: Grant
    Filed: December 12, 2011
    Date of Patent: May 28, 2013
    Assignees: The Research Foundation for the State University of New York, Cornell University
    Inventors: Li Niu, Zhen Huang, Hua Shi, John T. Lis
  • Patent number: 8399611
    Abstract: The template-fixed ?-hairpin peptidomimetics Cyclo(-Tyr-His-X-Cys-Ser-Ala-DPro-Dab-Arg-Tyr-Cys-Tyr-Gln-Lys-DPro-Pro), disulfide bond between Cys4 and Cys11, and pharmaceutically acceptable salts thereof, with X being Ala or Tyr, have CXCR4 antagonizing properties and can be used for preventing HIV infections in healthy individuals or for slowing and halting viral progression in infected patients; or where cancer is mediated or resulting from CXCR4 receptor activity; or where immunological diseases are mediated or resulting from CXCR4 receptor activity; or for treating immuno suppression; or for treating inflammation, or, in particular, for stem cell mobilization of peripheral blood stem cells and/or mesenchymal stem cell (MSC) and/or other stem cells which retention depend on the CXCR4-receptor.
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: March 19, 2013
    Assignees: Polyphor Ltd., Universitaet Zuerich
    Inventors: Daniel Obrecht, Frank Gombert, Steven J. Demarco, Christian Ludin, Jan Wim Vrijbloed, Kerstin Moehle, John-Anthony Robinson, Reshmi Mukherjee, Heiko Henze, Barbara Romagnoli
  • Patent number: 8383554
    Abstract: The protein CD1d binds self and foreign glycolipids for presentation to CD1-restricted T cells by means of TCR recognition, and activates TH1 and TH2 chemokines release. Accordingly, a variety of glycolipid ligands were attached to a microarray surface and their binding with CD1d investigated. An ?-galactosyl ceramide (?-GalCer) bearing a carbamate group at the 6?-OH position was tethered to the surface and the dissociation constant with CD1d determined. Competition assays were used to determine the dissociation constants (Ki) of the new and intact glycolipids. The para-fluoroheptaphenyl-modified ?-GalCer was found to bind most strongly with CD1d (Ki 0.14 ?M), two orders of magnitude stronger than ?-GalCer and more than three times more selective for IFN-? release.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: February 26, 2013
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Pi-Hui Liang
  • Patent number: 8377852
    Abstract: A method of preparing a substrate with a composition comprising (i) an organoborane initiator and (ii) a radical curable component disposed thereon includes the step of depositing the composition onto the substrate wherein at least one of (i) the organoborane initiator and (ii) the radical curable component is deposited onto the substrate in the form of a gradient pattern. An article comprises the substrate and the gradient pattern formed on the substrate. The gradient pattern is formed from a developed composition comprising the reaction product of (i) the organoborane initiator and (ii) the radical curable component. By forming the gradient pattern on the substrate, combinatorial and high-throughput methods of generating and testing the developed composition are possible, which enable characterization of the developed composition for various physical and chemical properties.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: February 19, 2013
    Assignee: Dow Corning Corporation
    Inventors: Dongchan Ahn, Kevin Wier
  • Patent number: 8348370
    Abstract: An array of electro-actuated fluid dispensers (200, 465, 500, 670) for metering out liquid reagents includes a support member (230); and a plurality of discrete electro-actuated fluid-dispensing die (220, 460) disposed on the support member (230), each of the die (220, 460) being configured to receive a supply of fluid (130, 300, 447, 454, 456, 665) being deposited thereon and dispense the fluid (130, 300, 447, 454, 456, 665) as needed for assay testing.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: January 8, 2013
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventor: Kevin F. Peters
  • Patent number: 8338165
    Abstract: A cell culture article including a substrate having nanoparticles on the substrate surface, the nanoparticle including: a polymer of formula (I) where (x), (y), (z), R, R?, R?, S, W, and X, are as defined herein. Methods for making the cell culture article or cell culture article and methods for performing an assay of a ligand with the article are also disclosed.
    Type: Grant
    Filed: November 25, 2009
    Date of Patent: December 25, 2012
    Assignee: Corning Incorporated
    Inventors: Wendy A. Baker, Bertrand De Lambert, David Henry, Odessa N. Petzold
  • Patent number: 8329009
    Abstract: Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi-well plate has a bottom region that is sized and shaped to simultaneous accommodate a sensing electrode and a pipette for delivering, e.g., test compounds, wash fluid, and optionally ligands. Such multi-well plates may be coupled with an instrument having a pipette head and an electrode plate. Such arrangement facilitates fluidic contact between cells and fluids provided via a pipette. It also facilitates washing of wells with buffers or other wash solutions to allow serial exposure of test cells to various reagents or other stimuli. Generally, the design allows control and test experiments to be performed on the same cell (or cells) in a single well.
    Type: Grant
    Filed: April 9, 2010
    Date of Patent: December 11, 2012
    Assignee: Molecular Devices, LLC
    Inventors: Yuri Vladimirovich Osipchuk, James Richard Wasson, Edward D. Verdonk
  • Patent number: 8293684
    Abstract: The present invention relates to labeling kits containing novel non-natural nucleotide monomers and to methods of making and using such compounds. The invention further relates to a method of detecting the presence of a nucleic acid, e.g., RNA, of interest in a sample, the method having the following steps: providing the sample; ligating a nucleic acid of interest with a labeling reagent according to the instant invention; providing a nucleic acid array having probes directed to the nucleic acid of interest; hybridizing the labeled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of the nucleic acid of interest.
    Type: Grant
    Filed: November 29, 2007
    Date of Patent: October 23, 2012
    Assignee: Exiqon
    Inventors: Peter Mouritzen, Christian Lomholt, Søren Morgenthaler Echwald, Mikkel Noerholm
  • Patent number: 8236791
    Abstract: Provided are methods of determining whether a compound is an inhibitor of an S100 protein. The methods utilize a biosensor that comprises the S100 protein that has a covalently bound fluorescent dye at an amino acid residue of the S100 protein that becomes less exposed to the aqueous solution upon activation of the S100 protein, where the fluorescent dye has decreased fluorescence when exposed to the aqueous solution than when protected from exposure to the aqueous solution. Also provided are methods of treating a subject having metastatic cancer.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: August 7, 2012
    Assignee: Albert Einstein College of Medicine of Yeshiva University
    Inventors: Anne Reba Bresnick, Sarah Claire Garrett
  • Patent number: 8101405
    Abstract: A biosensor article including a substrate having nanoparticles on the substrate surface, the nanoparticle including: a polymer of formula (I) where (x), (y), (z), R, R?, R?, S, W, and X, are as defined herein. Methods for making the biosensor article or cell culture article and methods for performing an assay of a ligand with the biosensor article are also disclosed.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: January 24, 2012
    Assignee: Corning Incorporated
    Inventors: Bertrand De Lambert, David Henry
  • Patent number: 8076467
    Abstract: The present invention relates to novel nucleic acid ligands or aptamers that bind to and inhibit the activation of the ?-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subtype of ionotropic glutamate receptors. Also disclosed is a novel combination of technologies, i.e., SELEX and laser pulse photolysis for the selection and screening of aptamers that inhibit receptor function and are useful therefore, in the treatment of diseases associated with excessive activation of ionotropic glutamate receptors.
    Type: Grant
    Filed: October 13, 2009
    Date of Patent: December 13, 2011
    Assignees: The Research Foundation of State University of New York, Cornell University
    Inventors: Li Niu, Zhen Huang, Hua Shi, John T. Lis