Abstract: A method for identifying a subject being at risk for or having a chronic inflammatory disease, fibrillinopathy, atherosclerosis, or coronary artery disease is provided. The method may include determining the concentration of GrA and/or GrB in a blood or serum sample from said subject; and comparing the concentrations to the corresponding concentration in a control sample, wherein an elevated concentration of GrA and/or GrB may be indicative of a chronic inflammatory disease, fibrillinopathy, atherosclerosis, or coronary artery disease. The method may further include identifying concentrations of fibrinogen, elastin and/or fibrillin.
Type:
Grant
Filed:
April 22, 2013
Date of Patent:
May 6, 2014
Assignee:
The University of British Columbia
Inventors:
David Granville, Rani Cruz, Ciara Chamberlain, Wendy Bolvin, Bruce McManus
Abstract: The invention provides an ELISA assay for the determination of serum mast cell ?-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of ?-tryptase, histamine and/or prostaglandin E2.
Abstract: The presently-disclosed subject matter provides methods for diagnosing a cardiovascular disease in a subject by determining an amount of one or more peptide biomarkers disclosed herein in a biological sample from the subject. The presently-disclosed subject matter further provides methods for determining treatment efficacy and/or progression of a cardiovascular disease in a subject by measuring amounts of one or more of the biomarkers in a biological sample from the subject. The presently-disclosed subject matter also provides antibodies and kits for measuring the biomarkers.
Type:
Grant
Filed:
April 18, 2008
Date of Patent:
April 22, 2014
Assignee:
University of Louisville Research Foundation, Inc.
Inventors:
Jon Klein, Michael L. Merchant, Rosemary Ouseph, Richard A. Ward
Abstract: Methods for the diagnosis, prognosis and prediction of sepsis in a subject using the expression levels of the biomarkers Triggering Receptor Expressed on Myeloid cells-1 (TREM-1) and TREM-like receptor transcript-1 (TLT1) as an indication of the condition of the patient, alone or in combination with further sepsis markers are disclosed. If the levels of the biomarkers indicate the presence of sepsis, the patient is treated for sepsis with an antibiotic and/or fluid resuscitation treatment.
Abstract: The present invention refers to a group of biomarkers and to non-invasive in vitro methods for the diagnosis or prognosis of endometriosis, as well as to a kit to perform said methods.
Type:
Grant
Filed:
November 26, 2008
Date of Patent:
March 25, 2014
Assignee:
Proteomike, S.L.
Inventors:
Daniel Nagore, Amagoia Ametzazurra, Antonio Martinez Martinez, Laureano Simón Buela
Abstract: The invention relates to novel markers of vascular inflammation and combinations thereof as diagnostic and prognostic tools in patients with cardiovascular diseases. The markers also act as tools that facilitate the selection of active ingredients for the treatment of such diseases, and finally act as starting points for the treatment of cardiovascular diseases. Furthermore, the invention relates to the creation of an individual risk profile of negative events that are associated with the progression of arteriosclerosis.
Abstract: The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers.
Type:
Grant
Filed:
July 18, 2013
Date of Patent:
March 18, 2014
Assignees:
Wallac Oy, The Fetal Medicine Foundation
Abstract: The present invention concerns the identification of biomarkers and groups or combinations of biomarkers that can be used for non-invasive diagnosis of intra-amniotic infection, and diagnostic assays using such biomarkers.
Abstract: The present invention relates to combinations of biomarkers and levels thereof that may be used, for example, in the determination of risk associated with the occurrence of a major adverse cardiac event (MACE) in a patient.
Type:
Grant
Filed:
June 25, 2013
Date of Patent:
February 25, 2014
Assignee:
Abbott Laboratories
Inventors:
Saul A. Datwyler, Jessie W. Shih, Robert C. Doss, Walter J. Keirans, Sudarshan Hebbar
Abstract: The present invention relates to proteins with pteridine glycosyltransferase activity and an analysis method using the same. Since the proteins glycate tetrahydrobiopterin selectively through an enzyme reaction, the method enables quantitative analysis of tetrahydrobiopterin and oxides thereof at the same time or quantitative analysis of tetrabiopterin selectively.
Type:
Grant
Filed:
March 30, 2009
Date of Patent:
February 25, 2014
Assignee:
Inje University Industry-Academic Cooperation Foundation
Abstract: The invention relates to a detection conjugate composed of a filament fragment, e.g. a cytoskeletal filament such as actin filaments or microtubules, and recognition elements bound to this fragment as well as kits comprising said detection conjugate and methods how to use said detection conjugate as well as the use for the detection of one or more compounds present within a sample, such as a biological sample.
Abstract: A method is provided for the diagnosis or prognosis of a disease state, involving the measurement of a parameter of discriminated dipeptidyl dipeptidases from a patient sample, and the correlation of the parameter with a disease.
Abstract: The present invention provides methods for the determination of the degree of molecular recognition of a protein with a ligand, including a first protein with a second protein. The methods may comprise determining the squared generalized order parameter (hereinafter, O) for at least one intramolecular bond of the first protein. The protein is then formed into a complex with a ligand. The value or values of O2 for the said at least one bond of the protein is then determined while the protein and the ligand are in the complex. The O value or values determined for the protein while the protein and the ligand are in a complex are compared or related to the O value or values determined for the uncomplexed protein.
Type:
Grant
Filed:
July 18, 2008
Date of Patent:
February 18, 2014
Assignee:
The Trustees of the University of Pennsylvania
Inventors:
Kathleen G. Valentine, Michael Marlow, Andrew J. Wand, Kendra K. Frederick
Abstract: The invention concerns the field of hemostasis, in particular blood coagulation disorders linked to an abnormal expression of tissue factor, and to physiopathological phenomena correlated with over-expression of the factor. The present invention provides a method for assaying the activity of circulating tissue factor in a biological sample. The method of the invention is carried out in vitro, in particular on a blood sample collected from a patient.
Type:
Grant
Filed:
July 16, 2009
Date of Patent:
February 11, 2014
Assignee:
Diagnostica Stago
Inventors:
Patrick Van Dreden, Aurelie Rousseau, Barry Woodhams
Abstract: The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers.
Type:
Grant
Filed:
January 26, 2009
Date of Patent:
February 11, 2014
Assignees:
PerkinElmer Health Sciences, Inc., Wallac Oy, The Fetal Medicine Foundation
Inventors:
Howard Cuckle, Kypros Nicolaides, Tarja Ahola, Leona Poon
Abstract: Methods and kits for binding and releasing biological targets, comprising, a binder comprising an environmentally reactive molecular switch that can switch between a high affinity state, to bind the target, to a low affinity state, to release the target.
Type:
Grant
Filed:
July 7, 2009
Date of Patent:
February 11, 2014
Assignee:
General Electric Company
Inventors:
Aaron Dulgar-Tulloch, Ernest William Kovacs, Evelina Roxana Loghin, Anup Sood
Abstract: The present invention provides a specific binding partner for holoTC having a specificity for holoTC over apoTC of at least 40-fold and an assay method for assaying for holoTC in a sample, the method comprising contacting the sample with a specific binding partner for holoTC and detecting the resultant conjugates.
Abstract: The present invention relates to an in vitro method for medical diagnosis, prognosis and therapy follow-up for patients having a cardiac disease or being suspected of developing or having a cardiac disease comprising the steps of: providing a sample of a patient having a cardiac disease or being suspected of developing or having a cardiac disease, determining amino-terminal proANp or partial peptides thereof having from 12 to 98 amino acids in said sample using at least one antibody that binds specifically to a partial sequence of amino-terminal proANP, attributing the determined amino-terminal proANP level or the level of partial peptides thereof to a clinical picture wherein the attribution is carried out independent of the BMI of the patient. The present invention further concerns a rapid test assay and a kit for conducting the method of the present invention and the use of antibodies suitable for the method and assays according to the present invention.
Type:
Grant
Filed:
May 6, 2008
Date of Patent:
February 11, 2014
Assignee:
B.R.A.H.M.S. GmbH
Inventors:
Andreas Bergmann, Jana Papassotiriou, Joachim Struck, Nils Morgenthaler, Stefan Anker