Patents Examined by M. Meller
  • Process for preparing a calcium-dependent oxygenase
    Patent number: 5023181
    Abstract: A process for preparing a Ca-dependent oxygenase having a continuously photogenic performance for a long time when it is continuously added to a substrate such as coelenterazine, which process comprises treating an apoaequorin obtained from natural photoprotein aequorin or an apoaequorin obtained by subjecting an apoaequorin to recombinant DNA technique, with a reducing agent such as 2-mercaptoethanol and reacting the resulting apoaequorin with a metal ion such as calcium ion, preferably in the presence of a substrate coelenterazine.
    Type: Grant
    Filed: July 13, 1988
    Date of Patent: June 11, 1991
    Assignee: Chisso Corporation
    Inventor: Satoshi Inouye