Abstract: An enzymatic assay composition for ethanol determination is provided. A compound of the formulaH.sub.2 N--(CH.sub.2).sub.n --CHR--CH.sub.2 --NH.sub.2whereR is H, CH.sub.3, or OH andN is 0-4acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.