Patents Examined by Manjunath N. Rao
  • Compositions of aminoacyl-tRNA synthetase and uses thereof
    Patent number: 7883866
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: February 8, 2011
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Human protein acyl transferases and methods of uses therefor
    Patent number: 7883860
    Abstract: The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefor. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: February 8, 2011
    Assignees: Washington University, The University of Iowa Research Foundation
    Inventors: Robert J. Deschenes, Sandra Lobo, John Swarthout, Maurine E. Linder
  • Albumin-based colloid composition having at least one protected thiol region, methods of making, and methods of use
    Patent number: 7884068
    Abstract: A composition comprising an albumin-based colloid composition having at least one protected thiol region, method of making the same, and method for use, including treating hypovolemic conditions such as capillary leak syndrome and shock, are disclosed.
    Type: Grant
    Filed: November 9, 2004
    Date of Patent: February 8, 2011
    Inventors: Ragheb A. Assaly, J. David Dignam, Joseph I. Shapiro
  • Large scale preparation of alpha-1 proteinase inhibitor and use thereof
    Patent number: 7879800
    Abstract: A process suitable for processing scaled-up amounts of source material in the range of tens of kilograms for the purification of alpha-1 proteinase inhibitor (API) from a mixture of unpurified proteins is provided. More particularly, a process for the purification of API from blood plasma or from plasma fractions to obtain pharmaceutical grade API on a commercial scale is provided. The API produced by the process is highly pure (at least 90% API out of the total protein) and highly active (at least 90% active API). Pharmaceutical compositions comprising the purified API and methods of using same are also described.
    Type: Grant
    Filed: September 22, 2004
    Date of Patent: February 1, 2011
    Assignee: Kamada Ltd.
    Inventor: Shabtai Bauer
  • Subtilases
    Patent number: 7875445
    Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
    Type: Grant
    Filed: February 23, 2010
    Date of Patent: January 25, 2011
    Assignee: Novozymes A/S
    Inventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
  • Enhanced cell-free synthesis of active proteins containing disulfide bonds
    Patent number: 7871794
    Abstract: Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: January 18, 2011
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Kurtis G. Knapp, James Robert Swarts
  • Process for removing fibronectin from plasma fractions
    Patent number: 7863420
    Abstract: The invention relates to a method for separating fibronectin from plasma fractions by adjusting a pH value of less than 5.4 such that fibronectin is precipitated and extracted from the solution.
    Type: Grant
    Filed: September 9, 2005
    Date of Patent: January 4, 2011
    Assignee: Biotest AG
    Inventors: Michael Kretschmar, Wolfgang Moeller
  • Compositions of aminoacyl-tRNA synthetase and uses thereof
    Patent number: 7858344
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: December 28, 2010
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Subtilases
    Patent number: 7858354
    Abstract: The present invention relates to novel subtilases from wild-type strains of Bacillus, especially the Bacillus strains ZI344, EP655, P203, EP63, ZI120, ZI130, ZI1342 and ZI140, and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry detergent or an automatic dishwashing detergent.
    Type: Grant
    Filed: January 30, 2009
    Date of Patent: December 28, 2010
    Assignee: Novozymes A/S
    Inventors: Preben Nielsen, Martin Borchert
  • Compositions of aminoacyl-tRNA synthetase and uses thereof
    Patent number: 7846689
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: December 7, 2010
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Recombinant vector capable of increasing secretion of Koji mold protease
    Patent number: 7842799
    Abstract: To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.
    Type: Grant
    Filed: September 19, 2006
    Date of Patent: November 30, 2010
    Assignees: Noda Institute for Scientific Research, Kikkoman Corporation
    Inventors: Osamu Hatamoto, Genryou Umitsüki, Masayuki Machida, Motoaki Sano, Akimitsu Tanaka, Chitoshi Oka, Hiroshi Maeda, Hitoshi Tainaka, Touru Ito, Tomomi Uchikawa, Tsutomu Masuda, Kenichiro Matsushima
  • Compositions of aminoacyl-tRNA synthetase and uses thereof
    Patent number: 7838265
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: November 23, 2010
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Catheter composition and uses thereof
    Patent number: 7829082
    Abstract: A composition useful for removal of fibrin-bound blood clots from a catheter comprises water, a fibrinolytically effective amount of a plasminogen activator, and a preservatively effective amount of a bacteriostatic organic alcohol. The composition does not comprise a chelating agent.
    Type: Grant
    Filed: June 23, 2006
    Date of Patent: November 9, 2010
    Assignee: Genentech, Inc.
    Inventor: Charles P. Semba
  • Compositions of aminoacyl-tRNA synthetase and uses thereof
    Patent number: 7829310
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
    Type: Grant
    Filed: March 15, 2010
    Date of Patent: November 9, 2010
    Assignee: Ambrx, Inc.
    Inventors: Andrew Paulsel, Ho Sung Cho
  • Recombinant microorganism comprising inactivation of the AprX gene
    Patent number: 7829322
    Abstract: A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided. Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
    Type: Grant
    Filed: December 20, 2005
    Date of Patent: November 9, 2010
    Assignees: Kao Corporation, Shinshu University
    Inventors: Takeko Kodama, Keiji Endo, Katsuya Ozaki, Junichi Sekiguchi
  • In vivo incorporation of alkynyl amino acids into proteins in eubacteria
    Patent number: 7824893
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthoghonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.
    Type: Grant
    Filed: September 20, 2005
    Date of Patent: November 2, 2010
    Assignee: The Scripps Research Institute
    Inventors: Alexander Deiters, Peter G. Schultz
  • Schizochytrium fatty acid synthase (FAS) and products and methods related thereto
    Patent number: 7820407
    Abstract: Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.
    Type: Grant
    Filed: May 1, 2009
    Date of Patent: October 26, 2010
    Assignee: Martek Biosciences Corporation
    Inventors: James G. Metz, Craig A. Weaver, Jerry Kuner
  • Site-specific incorporation of redox active amino acids into proteins
    Patent number: 7811801
    Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
    Type: Grant
    Filed: December 17, 2008
    Date of Patent: October 12, 2010
    Assignee: The Scripps Research Institute
    Inventors: Lital Alfonta, Peter G. Schultz, Zhiwen Zhang
  • Genetically encoded fluorescent coumarin amino acids
    Patent number: 7807411
    Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl) ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
    Type: Grant
    Filed: May 21, 2007
    Date of Patent: October 5, 2010
    Assignee: The Scripps Research Institute
    Inventors: Jiangyun Wang, Jianming Xie, Peter G. Schultz
  • Family GH 61 polypeptides
    Patent number: 7803590
    Abstract: The present invention relates to use of an anti-staling GH-61 polypeptide for preparing an edible product.
    Type: Grant
    Filed: November 12, 2009
    Date of Patent: September 28, 2010
    Assignee: Novozymes A/S
    Inventors: Kirk Matthew Schnorr, Sara Landvik, Tina Spendler, Lars Lehmann Hylling Christensen