Patents Examined by Manjunath Rao
  • Patent number: 9816127
    Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: November 14, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Fen Huang, Dieter Klaubert, John Shultz, Wenhui Zhou
  • Patent number: 9731052
    Abstract: The invention provides a method for the controlled assembly of layered silk fibroin coatings using aqueous silk fibroin material. The methods described herein can be used to coat substrates of any material, shape, or size. Importantly, the described methods enable control of the biomaterial surface chemistry, thickness, morphology and structure using layered thin film coatings, or bulk coatings. Furthermore, the methods can be performed in all water and do not require intensive chemical processing enabling controlled entrapment of labile molecules such as, drugs, cytokines, and even cells or viruses to generate functional coatings that can be used in a variety of applications.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: August 15, 2017
    Assignee: Trustees of Tufts College
    Inventors: David L. Kaplan, Xianyan Wang
  • Patent number: 9688970
    Abstract: A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: June 27, 2017
    Assignee: Kentucky BioProcessing, Inc.
    Inventors: Greg Pogue, Ernie Hiatt, Romy Kandzia, Stefan Werner, Frank Thieme, Tsafrir Mor, Steven Hume
  • Patent number: 9624281
    Abstract: In one aspect the present invention is directed to mutant NGAL proteins that have the ability to bind to siderophores, such as enterochelin, and to chelate and transport iron, and that are excreted in the urine. Such NGAL mutants, and complexes thereof with siderophores, can be used to clear excess iron from the body, for example in the treatment of iron overload. The NGAL mutants of the invention also have antibacterial activity and can be used in the treatment of bacterial infections, such as those of the urinary tract.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: April 18, 2017
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jonathan Barasch, Andong Qiu
  • Patent number: 9458222
    Abstract: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: October 4, 2016
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Leopold Grillberger, Manfred Reiter, Daniel Fleischanderl, Gregor Bramberger
  • Patent number: 9447401
    Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: September 20, 2016
    Assignee: Halozyme, Inc.
    Inventors: Ge Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
  • Patent number: 9447385
    Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: September 20, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
  • Patent number: 9440006
    Abstract: A method for forming a porous tissue material.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: September 13, 2016
    Assignee: FUJIFILM Corporation
    Inventor: Huibert Van Boxtel
  • Patent number: 9434919
    Abstract: The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: September 6, 2016
    Assignee: Codexis, Inc.
    Inventors: Lorand Szabo, Zsolt Molnar, Attila Laszlo Nemeth
  • Patent number: 9434915
    Abstract: The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides automatic dishwashing compositions comprising this serine protease variant.
    Type: Grant
    Filed: February 5, 2015
    Date of Patent: September 6, 2016
    Assignee: Danisco US Inc.
    Inventors: David A. Estell, Frits Goedegebuur, Ayrookaran J. Poulose
  • Patent number: 9422541
    Abstract: An apparatus for the extracorporeal treatment of blood comprising an extracorporeal blood circuit (2), a pump (6) configured to provide fluid displacement within the extracorporeal blood circuit, and a reaction chamber (8) connected to the extracorporeal blood circuit and configured to receive blood or plasma from the circuit and treat the blood or plasma. The reaction chamber comprises an protease enzyme immobilized to a support, in which the protease enzyme is specific for, and capable of irreversibly cleaving, a human C5a present in the blood or plasma, wherein the abundance of the human C5a in the treated blood or plasma is less than that in the untreated blood or plasma. The apparatus finds utility in the extracorporeal treatment of blood from patients with inflammatory conditions, especially auto-immune disease and sepsis.
    Type: Grant
    Filed: June 25, 2014
    Date of Patent: August 23, 2016
    Assignee: University of Limerick
    Inventors: Jakki Cooney, Todd Fumio Kagawa, Edmond Magner
  • Patent number: 9416355
    Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.
    Type: Grant
    Filed: January 4, 2011
    Date of Patent: August 16, 2016
    Assignees: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/S
    Inventors: Carsten Andersen, Randall Deinhammer, Thomas Agersten Poulsen, Miguel Duarte Toscano, Peter Kamp Hansen, Henrik Friis-Madsen, Anders Viksoe-Nielsen, Signe Eskildsen Larsen, Lars L. H. Christensen
  • Patent number: 9410135
    Abstract: The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant Trichoderma reesei endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: August 9, 2016
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Harshal Akshay Chokhawala, Tae-Wan Kim, Harvey W. Blanch, Douglas S. Clark
  • Patent number: 9410166
    Abstract: The invention relates to suitable candidate alcohol dehydrogenase (ADH) enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: August 9, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Sriram Satagopan, Daniel P. O'Keefe, Janardhan Gude
  • Patent number: 9388403
    Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.
    Type: Grant
    Filed: May 30, 2012
    Date of Patent: July 12, 2016
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
  • Patent number: 9381282
    Abstract: The invention provides methods, compositions, and devices for promoting adhesion or migration of endothelial cell.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: July 5, 2016
    Assignee: University of Utah Research Foundation
    Inventors: Dean Y. Li, Brent D. Wilson, Lise Sorensen Brunhart
  • Patent number: 9382528
    Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: July 5, 2016
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
  • Patent number: 9382422
    Abstract: The invention provides tissue repair compositions and methods of making the tissue repair compositions. Also featured are methods of treatment using the tissue repair compositions and articles of manufacture that include the tissue repair compositions.
    Type: Grant
    Filed: July 9, 2008
    Date of Patent: July 5, 2016
    Assignee: LifeCell Corporation
    Inventors: Rick T. Owens, Wendell Sun
  • Patent number: 9359584
    Abstract: Provided in part herein are compositions that include isolated microbial enzymes, such as amylases, lipases, and proteases that are useful as detergent additives. Also provided are methods of isolating amylases, lipases, and proteases from microbes, as well as methods of using these enzymes as detergent additives, and for stain removal.
    Type: Grant
    Filed: July 24, 2010
    Date of Patent: June 7, 2016
    Assignee: WEST BENGAL UNIVERSITY OF TECHNOLOGY
    Inventor: Shaon Ray Chaudhuri
  • Patent number: 9353358
    Abstract: The subject of the invention is the ribonuclease which cleaves RNA strand in DNA-RNA hybrids, wherein ribonuclease comprises fusion protein comprising catalytic domain of RNase HI (RNase HI) or derivative thereof with a zinc finger DNA-RNA hybrid binding domain, and wherein the zinc finger binding domain has the ability to bind to specific sequences in the DNA-RNA hybrid. The invention also relates to new methods for determination of the sequence preference of DNA-RNA hybrid binding protein(s) or its domain(s) and allows determining the sequence recognized by sequence specific binding protein in the DNA-RNA hybrid.
    Type: Grant
    Filed: December 3, 2013
    Date of Patent: May 31, 2016
    Assignee: MIEDZYNARODOWY INSTYTUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ
    Inventors: Janusz Marek Bujnicki, Agata Agnieszka Sulej, Krzysztof Jerzy Skowronek, Marcin Nowotny