Abstract: A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaing the sugar-alcohols in the effluent out of the column, a column filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.

Abstract: Novel DNA probe sequences for detection of CMV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.

Abstract: Methods are provided for detecting the presence of a gene for torsion dystonia in a human subject. Furthermore, a haplotype which is associated with torsion dystonia is disclosed. Methods and kits for the detection of torsion dystonia in a subject are additionally provided.

Abstract: An oligonucleotide or analog thereof including a single cytidine residue at a selected position and having one or more cytidine residues which are 5-methyl substituted. The cytidine nucleus can be selectively transaminated to include an aminoalkyl or carboxyalkyl group as a linker for other functional groups which can be used to form DNA duplexes and triplexes.

Abstract: The invention relates to a hybridization probe for detecting the existence of bacteria in a sample. It consists of one of the two pentadecadeoxyribonucleotides of sequence(5')d - AAG GAG GTG ATC CAG (3'),(5')d - CTG GAT CAC CTC CTT (3').

Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.

Abstract: Isolated nucleic acid molecules capable of hybridizing to sequences of Candida albicans along with methods utilizing such probes for the detection of Candida albicans in clinical and other biological samples.

Abstract: Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.

Abstract: The present invention relates generally to the identification and characterization of new genes and proteins. More particularly, the present invention relates to the discovery of novel members of the nuclear hormone receptor superfamily and cDNA clones thereof. The family members are designated as H-2RIIBP (or RXR.sub..beta., retinoid x receptors). These proteins bind selectively only to the native region II sequence of the conserved major histocompatibility complex class I regulatory element (MHC CRE). Sequences homologous to the H-2RIIBP gene are found in the nuclear receptor family including: retinoic acid receptors (RAR), estrogen receptors (ER), thyroid hormone receptors (TR), (COUP-TF), and other RXR isoformes.This invention also provides for a diagnostic test which determines the nature and progression of a human tumor by measuring the quantity and quality of H-2RIIBP gene dosage or expression.

Abstract: The invention relates to synthetic oligonucleotides and more particularly to the determination of nucleotide sequences of synthetic oligonucleotides having non-phosphodiester internucleotide linkages. The invention provides a method for sequencing such modified synthetic oligonucleotides.

Abstract: Methods and compositions for forming ligation product hybridized to a nucleic acid template. The ligation product is formed by contacting a nucleic acid template with a primer capable of hybridizing to the template to form a primed template. The primed template is then contacted with a pool of random extension oligonucleotides of length N and an enzyme system. The enzyme system is capable of covalently linking the primer to an extension oligonucleotide adjacently hybridized to it and one or more other extension oligonucleotides adjacently hybridized to the ligation product defined by covalently linked ligation primer and one or more extension oligonucleotides. When covalently linked, the ligation product is hybridized to the nucleic acid template. Modifications permit the determination of the nucleotide sequence of one or more members of a first set of target nucleotide residues in the nucleic acid template that are spaced at intervals of N nucleotides.

Abstract: Nucleic acid probes are described for detecting yeasts capable of causing Candida infection, specifically yeasts of the genera Candida, Torulopsis, and Yarrowia. The preferred probes are complementary to ribonucleic acid sequences unique to specific Candida species, or groups of such species, and as such can detect the rRNA, rDNA, or polymerase chain reaction amplification products of these genes. Methods for detecting the etiological agent of human Candida fungemia utilizing these probes are also provided.

Abstract: The present invention provides an isolated nucleic acid encoding an approximately 120-128 kilodalton antigen of Helicobacter pylori, or an antigenic fragment thereof, wherein the antigen is associated with peptic ulceration. The present invention also provides methods of detecting the presence of a Helicobacter pylori strain possessing the 120-128 kilodalton antigen in a subject, comprising the steps of contacting an antibody-containing sample from the subject with a detectable amount of the tagA antigen or antigenic fragment of the present invention and detecting the reaction of the antigen or fragment and the antibody. A mutant H. pylori not expressing a functional tagA antigen is also provided.

Abstract: Human erythroid p55, an abundantly palmitoylated erythrocyte membrane protein, has been identified, cloned and sequenced. p55 appears to be ubiquitously expressed in human tissues and contains both an SH3 motif and an enzymatically active guanylate kinase domain. The presence of the SH3 motif indicates a possible role in the suppression of tyrosine kinase activity and the guanylate kinase domain may play a role in signal transduction and tissue proliferation by modulating guanine nucleotide levels. Localized to the Xq24-qter region of the X chromosome, abnormalities in p55 appear in patients suffering from hemolytic anemia and Dyskeratosis congenita. With the identification and sequencing of p55, nucleic acid probes and anti-p55 antibodies can be used in a variety of hybridization and immunological assays to screen for and detect p55 defects. Conventional and gene techniques can also be developed to treat p55 deficiencies and abnormalities.

Abstract: Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.

Abstract: A species-specific DNA-DNA hybridization probe prepared using an isolated whole chromosome of a lower eukaryote as template. The probe is useful for the detection of conspecificity in lower eukaryotes selected from the group consisting of yeast and molds.

Abstract: A human prohibitin gene, a protein coded for by said gene, a gene analysis reagent to be used with them, and a quantitative determination of prohibitin in a biological sample by an immunological technique with the use of an antihuman prohibitin antibody and a method for analyzing a prohibitin gene of a human tissue for the occurrence of mutation by the PCR method with the use of oligonucleotides having partial base sequences of said gene as primers.

Abstract: The present invention relates to an isolated nucleic acid fragment comprising a nucleic acid sequence encoding an apamin receptor protein, or biologically active fragment thereof.

Abstract: Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

Abstract: Novel shuttle vectors which are useful as shuttle vectors among microorganisms belonging to Escherichia coli, Gluconobacter and Acetobacter comprising one or more marker genes, a replication origin functional in Escherichia coli, a replication origin functional in Gluconobacter oxydans and a Mob site.