Patents Examined by Mary E. Ceperley
  • Patent number: 7364911
    Abstract: The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.
    Type: Grant
    Filed: May 14, 2002
    Date of Patent: April 29, 2008
    Inventors: Rudolf H. Aebersold, Huilin Zhou, Beate Rist, George J. Vella, Subhasish Purkayastha, Sasi Pillai
  • Patent number: 7229782
    Abstract: The present invention is directed antibodies specific to multiple beta blockers, as well as immunogens used to produce the antibodies and immunoassay kits and methods for using the antibodies.
    Type: Grant
    Filed: August 3, 2004
    Date of Patent: June 12, 2007
    Assignee: LabOne, Inc.
    Inventor: Liuming Yu
  • Patent number: 7220842
    Abstract: Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.
    Type: Grant
    Filed: April 5, 2004
    Date of Patent: May 22, 2007
    Assignee: Dade Behring Inc.
    Inventors: Yi-Feng Zheng, Pratap Singh, Dorota Bolle, Hshiou-Ting Liu
  • Patent number: 7217575
    Abstract: A covalent conjugate of a 4?-hydroxyazobenzene-2-carboxylic acid derivative (HABA) and an avidin-type molecule, of the formula: wherein A is (CH2)n or —CH?CH—, wherein n is an integer from 0–10; B is (CH2)n wherein n is an integer from 2 to 10; m is zero or 1; and Av is the residue of an avidin-type molecule selected from the group comprising native egg-white avidin, recombinant avidin, deglycosylated avidins, bacterial streptavidin, recombinant streptavidin, truncated streptavidin and other derivatives of said avidin-type molecules. These HABAylated avidins are red colored in the quinone configuration and can be used in many applications in the avidin-biotin technology.
    Type: Grant
    Filed: July 23, 2003
    Date of Patent: May 15, 2007
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Meir Wilchek, Edward A Bayer, Heike Hofstetter, Margherita Morpurgo
  • Patent number: 7214546
    Abstract: Chemiluminescent substrate delivery systems comprising a conjugate of a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used in assays to detect the presence of an analyte (e.g., an enzyme, an antibody, an antigen or a nucleic acid) in a sample.
    Type: Grant
    Filed: January 8, 2002
    Date of Patent: May 8, 2007
    Assignee: Applera Corporation
    Inventor: Alison L. Sparks
  • Patent number: 7211445
    Abstract: A method is provided for covalently linking carbohydrates, proteins, nucleic acids, and other biomolecules under neutral conditions, using a Diels-Alder cycloaddition reaction. In an example, activated carbon-carbon double bonds were attached to free amino sites of a carrier protein, and a conjugated diene was attached to a carbohydrate hapten. Spontaneous coupling of the carbohydrate and the protein components under very mild conditions provided glycoconjugates containing up to 37 carbohydrate hapten units per carrier protein molecule. The method is also applicable to the immobilization of biomolecules on gel or solid supports. The conjugated products are useful as immunogens and as analytical and diagnostic reagents.
    Type: Grant
    Filed: October 22, 2003
    Date of Patent: May 1, 2007
    Assignee: The United States of America as Represented by the Secretary of the Department of Health and Human Services
    Inventor: Vince Pozsgay
  • Patent number: 7208322
    Abstract: The present invention provides a sensor surface comprising: a substrate coated with a free electron metal; and a matrix layer disposed on the free electron metal, wherein the matrix layer comprises an organic compound having a boronic acid complexing moiety. The matrix is preferably a self-assembled monolayer (SAM), a mixed self-assembled monolayer (mSAM), or combinations thereof.
    Type: Grant
    Filed: April 2, 2002
    Date of Patent: April 24, 2007
    Assignee: Agilent Technologies, Inc.
    Inventors: Mark L. Stolowitz, Jean P. Wiley, Guisheng Li, Kevin Lund
  • Patent number: 7208299
    Abstract: The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
    Type: Grant
    Filed: December 30, 2003
    Date of Patent: April 24, 2007
    Assignee: Universtiy of Utah Research Foundation
    Inventors: Karin D. Caldwell, Patrick A. Tresco, Jennifer Neff
  • Patent number: 7205162
    Abstract: Artificial antibodies or antibody mimics are described. They consist of polymers that carry specific binding sites mimicking the properties of antibodies. There is also described a method for producing artificial antibodies, in which polymerisable monomers carrying functional groups and crosslinking monomers are polymerised in the presence of a print molecule and subsequently the print molecule is removed leaving specific binding sites complementary to the print molecules. There are also described methods for determination and isolation of organic molecules using the artificial antibodies as well as therapeutic and diagnostic methods using these antibodies.
    Type: Grant
    Filed: May 6, 1999
    Date of Patent: April 17, 2007
    Inventors: Klaus Mosbach, Georg Vlatakis, Lars I. Andersson, Ralf Müller
  • Patent number: 7205161
    Abstract: Sensors for determining the presence and concentration of biomolecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface modified with a hydrophobic polymer segment, attached to which is a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, which allows for controlled architecture polymers to modify the surface of the substrate, and the use of monomers possessing functional groups which do not require activation prior to probe attachment. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.
    Type: Grant
    Filed: January 10, 2002
    Date of Patent: April 17, 2007
    Assignee: Symyx Technologies, Inc.
    Inventors: Gerrit Klaerner, Didier Benoit, Dominique Charmot, Srinivas Nomula, Marcelo Eduardo Piotti, Laura T. Mazzola
  • Patent number: 7202348
    Abstract: The present invention provides synthetic immunochemical haptens for the generation of antibodies that are designed to recognize the common molecular features of d-methamphetamine-like abused stimulants with insignificant cross-reactivity to endogenous substrates (e.g. dopamine) or over-the-counter medications (e.g. 1-methamphetamine, pseudoephedrine, phenylpropanolamine and ephedrine). These monoclonal antibodies and their antigen binding fragments are useful in treatment plans for recovering addicts, in emergency room settings for rapidly reversing a drug overdose, in protection of fetuses from drug-abusing pregnant mothers or in a psychiatric setting to reduce the exacerbation of psychotic disorders caused by stimulant drugs.
    Type: Grant
    Filed: September 26, 2002
    Date of Patent: April 10, 2007
    Assignee: The University of Arkansas for Medical Sciences
    Inventors: Samuel M. Owens, Frank Ivy Carroll, Philip Abraham
  • Patent number: 7202092
    Abstract: Derivatives and conjugates of indinavir for generation of antibodies and labeled conjugates for use for detection of indinavir in biological samples. The derivatives are synthesized out of the indane ring hydroxyl group or the pyridine ring nitrogen of indinavir. Also disclosed is synthesis of a major metabolite of indinavir (M6) in a single step from indinavir using palladium catalyst and hydrogen gas. Indinavir M6 has been extended to synthesize various analogs of indinavir with suitable functional groups. These derivatives are useful in the development of indinavir immunogens, antibodies, and labeled conjugates in the development of indinavir immunoassays.
    Type: Grant
    Filed: November 12, 2004
    Date of Patent: April 10, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Mitali Ghoshal, Gerald Sigler, Aniruddha P. Patwardhan
  • Patent number: 7198958
    Abstract: A fluorescent label compound of formula where Z is a reactive group that can be used for covalent coupling of the fluorescent label compound to other molecule. Either the fluorophore is a dipyrrometheneboron difluoride dye and Y is a water-solubilizing moiety, or Y is —CH2CH2SO3? X+ where X+ is a cation. The use of the compounds in bianalytical assays and cytological or histological staining methods, and a method for increasing the hydrophilicity of fluorescent compounds, are also disclosed.
    Type: Grant
    Filed: July 1, 2002
    Date of Patent: April 3, 2007
    Assignee: Arctic Diagnostics Oy
    Inventors: Niko Meltola, Aleksi Soini
  • Patent number: 7195882
    Abstract: The present invention relates to monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof. The present invention further relates to buprenorphine metabolite conjugates for the production of monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof and hybridoma cells that produce the monoclonal antibodies. The invention also relates to immunoassay methods for determining buprenorphine and/or one or more buprenorphine metabolites in a sample using the novel antibodies and conjugates of the present invention.
    Type: Grant
    Filed: June 3, 2003
    Date of Patent: March 27, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Richard T. Root, Mitali Ghoshal
  • Patent number: 7193065
    Abstract: Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for HIV protease inhibitors. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group common to all HIV protease inhibitors, e.g., saquinavir, nelfinavir, indinavir, amprenavir, ritonavir, lopinavir, and atazanavir.
    Type: Grant
    Filed: September 24, 2003
    Date of Patent: March 20, 2007
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Gerald F. Sigler, Raymond A. Hui, Ina Deras, Richard Terry Root, Mitali Ghoshal, Erasmus Huber, Herbert W. Von Der Eltz, Sigrun Metz, Peter Kern
  • Patent number: 7192719
    Abstract: Whether or not a sample of skin or of a skin equivalent contains such amount of a papillary fibroblast population as to be considered a normal skin is determined by labelling said skin or skin equivalent with at least one antibody specific for papillary fibroblasts and evaluating the extent of such labelling as a marker for skin or skin equivalent quality.
    Type: Grant
    Filed: July 15, 2003
    Date of Patent: March 20, 2007
    Assignee: L'Oreal
    Inventors: Daniel Asselineau, Arnold Caplan
  • Patent number: 7186518
    Abstract: New derivatives of FK 506 are disclosed. These new derivatives and other derivatives that are useful for determining the levels of FK 506 in a sample are also provided as are assay procedures and kits for use in determining the levels of FK 506 or other macrophilin binding substances in blood, particularly un-extracted blood in the presence of specific binding proteins for FK 506.
    Type: Grant
    Filed: November 21, 2003
    Date of Patent: March 6, 2007
    Assignee: Dade Behring Inc.
    Inventors: Chengrong Wang, Tie Q. Wei, Zhu Teng
  • Patent number: 7183392
    Abstract: Heterofunctional crosslinking groups are provided having the formula: wherein W is a covalent core component; L1, L2 and L3 are independently linking groups; X is a non-covalent or reversibly covalent protein tag binder; Y is a activatable covalent linking group; and Z is a protected or unprotected covalent crosslinking group.
    Type: Grant
    Filed: March 27, 2001
    Date of Patent: February 27, 2007
    Assignee: Zyomyx, Inc.
    Inventors: Peter Wagner, Lifu Ma, Steffen Nock, David Wilson, Jens Sydor
  • Patent number: 7183116
    Abstract: The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.
    Type: Grant
    Filed: May 14, 2001
    Date of Patent: February 27, 2007
    Assignees: The Institute For Systems Biology, University of Washington, Applera Corporation
    Inventors: Rudolf H. Aebersold, Huilin Zhou, Beate Rist, George J. Vella, Subhasish Purkayastha, Sasi Pillai
  • Patent number: RE39663
    Abstract: The invention provides compositions electron-deficient nitrogen heterocycle-substituted fluorescein dyes and methods in which the dyes are conjugated to substrates and used as detection labels in molecular biology experiments. The electron-deficient nitrogen heterocycles include pyridine, quinoline, pyrazine, and the like. Substrates include polynucleotides, nucleosides, nucleotides, peptides, proteins, carbohydrates, and ligands.
    Type: Grant
    Filed: April 6, 2004
    Date of Patent: May 29, 2007
    Assignee: Applera Corporation
    Inventors: Krishna G. Upadhva, Steven M. Menchen, Weiguo Zhen