Abstract: A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the cabohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, the .alpha. or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligosaccharide compound being separated from the reaction mixture.

Abstract: A method for improving the yield of heterologous protein such as ricin A toxin, produced by recombinant bacteria by supplementing the nutrient medium in which the bacteria are grown with an ethanol and/or amino acid mixture during the terminal phase of the cultivation. Also disclosed is a method for improving the yield of heterologous proteins under the control of the PL promoter.

Abstract: Glucose dehydrogenase having the following biochemial properties:(a) enzymatic action: catalyzes a reaction which generates glucono-.delta.-lactone and reduced NADP from glucose and NADP;(b) substrate specificity: has substrate specificity on glucose and no substrate specificity on 2-deoxyglucose;(c) optimum pH: pH 6-8,(d) optimum temperature: approximately 55.degree. C.,(e) pH-stability: stable at pH 6.0-7.5,(f) molecular weight: 11.times.10.sup.4 .+-.11000,(g) Km-value: 2.6.times.10.sup.-3 .+-.2.6.times.10.sup.-4 M(glucose) 4.2.times.10.sup.-6 35 4.2.times.10.sup.-7 M(NADP) and(h) isoelectric point: 4.9.+-.0.5,comprises culturing a glucose dehydrogenase-producing microorganism Cryptococcus uniguttulatus Y 0033 FERM P-8709, now FERM BP-1352 in a nutrient medium and isolating glucose dehydrogenase thus produced from the cultured medium.

Abstract: A process for preparing an enzyme preparation useful for interesterification in the presence of little water. The process comprises drying a hydrated substance having lipase-activity while contacting the substance with a fatty acid derivative.