Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.

Abstract: The present inventions relate to a breeding system for the production of polyploid maize seeds, maize plants, or maize plant parts where cycles of meiosis, syngamy, and selection are used for interpopulation improvement of progenitor lines, and sexual polyploidization occurs during hybrid production by inducing clonal gamete formation in the parents that are to be crossed. Reciprocal recurrent selection can be used to inform selection of candidate maize lines that are either advanced to a gene editing or genetic modification system or crossed and selected to induce clonal gamete formation by arresting meiotic recombination and chromosome reduction. Crosses of parent maize plants bearing clonal gametes are planned and executed based upon predicted heterotic performance at the polyploid level. The final product is a homogeneous population of hybrid polyploid maize seed, or derivative thereof, bearing both parents' complete nuclear genomes.

Abstract: The invention relates to the alfalfa variety designated R411M104. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R411M104. Also provided by the invention are tissue cultures of the alfalfa variety R411M104 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R411M104 with itself or another alfalfa variety and plants produced by such methods.

Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.

Abstract: The invention relates to the novel cotton variety designated 20R816B3TXF. Provided by the invention are the seeds, plants, plant parts and derivatives of the cotton variety 20R816B3TXF. Also provided by the invention are methods of using cotton variety 20R816B3TXF and products derived therefrom. Still further provided by the invention are methods for producing cotton plants by crossing the cotton variety 20R816B3TXF with itself or another cotton variety and plants and seeds produced by such methods.

Abstract: A hybrid pepper plant designated ALTAMURA is disclosed. The disclosure relates to the seeds of pepper hybrid ALTAMURA, to the plants and plant parts of hybrid pepper ALTAMURA, and to methods for producing a pepper plant by crossing the hybrid pepper ALTAMURA with itself or another pepper plant.

Abstract: A new and distinct lettuce variety NUN 08224 LTL is disclosed, as well as seeds and plants and heads or leaves thereof. NUN 08224 LTL is a Batavia lettuce variety, comprising resistance to Downy Mildew (Bremia lactucae) Isolates B1:16-37EU, Nasonovia ribisnigri Biotype Nr:0, and Lettuce Mosaic Virus (LMV) Pathotype II.

Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using genes derived from wild Glycine. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.

Abstract: The present invention provides novel garden bean cultivar Thompson and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a bean plant by crossing the bean plants of the invention with themselves or another bean plant. The invention also provides bean plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

Abstract: The present disclosure relates to compositions and methods related to tissue-specific promoters and their uses in plants, including tobacco and cannabis. The provided trichome-specific promoters enable the expression of heterologous polynucleotides in trichome tissues.

Abstract: Compositions and methods for genetically modifying the production levels of nicotine and other alkaloids in plants are provided.

Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

Abstract: This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. Methods to create or alter pesticidal proteins are provided for altered or enhanced pesticidal activity.

Abstract: Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: A soybean cultivar designated 12130154 is disclosed. The invention relates to the seeds of soybean cultivar 12130154, to the plants of soybean cultivar 12130154, to the plant parts of soybean cultivar 12130154, and to methods for producing progeny of soybean cultivar 12130154. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 12130154. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 12130154, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 12130154 with another soybean cultivar.

Abstract: The present invention discloses a method for screening for and identifying a desirable plant improving trait, said method comprises steps of: (a) obtaining genetic material from a sampling of a predefined source and (b) constructing an expression library from said genetic material. The aforementioned method further comprises steps of: (c) producing plants transformed with said expression library at a transformation efficiency of at least 0.05%-30%, representing at least 102-1010 trangenes; (d) screening for transformed plants expressing said desirable trait; and (e) identifying said transgene of said transformed plants expressing said desirable trait.

Abstract: A novel soybean variety, designated 5PLKD89 is provided. Also provided are the seeds of soybean variety 5PLKD89, cells from soybean variety 5PLKD89, plants of soybean 5PLKD89, and plant parts of soybean variety 5PLKD89. Methods provided include producing a soybean plant by crossing soybean variety 5PLKD89 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PLKD89, methods for producing other soybean varieties or plant parts derived from soybean variety 5PLKD89, and methods of characterizing soybean variety 5PLKD89. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PLKD89 are further provided.

Abstract: A novel soybean variety, designated 5PNMK70 is provided. Also provided are the seeds of soybean variety 5PNMK70, cells from soybean variety 5PNMK70, plants of soybean 5PNMK70, and plant parts of soybean variety 5PNMK70. Methods provided include producing a soybean plant by crossing soybean variety 5PNMK70 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PNMK70, methods for producing other soybean varieties or plant parts derived from soybean variety 5PNMK70, and methods of characterizing soybean variety 5PNMK70. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PNMK70 are further provided.

Abstract: Pesticidal proteins exhibiting inhibitory, suppressive, and toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4064 and TIC4064 amino acid sequence variants. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4064 and TIC4064 amino acid sequence variant pesticidal proteins are also provided.

Abstract: A novel maize variety designated 1PSSQ17 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PSSQ17 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PSSQ17 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PSSQ17 or a locus conversion of 1PSSQ17 with another maize variety.