Patents Examined by Melanie Brown
  • Patent number: 10570361
    Abstract: Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
    Type: Grant
    Filed: May 24, 2017
    Date of Patent: February 25, 2020
    Assignees: President and Fellows of Harvard College, Universität Augsburg
    Inventors: David A. Weitz, Thomas Franke, Achim Wixforth, Lothar Schmid, Jeremy Agresti, Adam R. Abate
  • Patent number: 10564157
    Abstract: An analyte capture device and related systems and methods are provided. The analyte capture device includes a glass material, an outer surface defined by the glass material, and a plurality of pores formed in the glass material along at least a portion of the outer surface. The analyte capture device is exposed to an environment containing an analyte for a period of time such that the analyte is captured within the plurality of pores of the glass material. The concentration of the analyte within the glass material is greater than a concentration of the analyte within the environment. The analyte capture device is then removed from the environment, and a property of the analyte within the analyte capture device is detected via an analyte detection system.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: February 18, 2020
    Assignee: Corning Incorporated
    Inventors: Jeffrey Stapleton King, Prantik Mazumder, Elaine Victoria Seraya
  • Patent number: 10564152
    Abstract: Methods for detecting antigen-specific antibodies in a biological sample are described. The disclosed methods can be used for the diagnosis of a variety of autoimmune and infectious diseases. The methods use a neodymium magnet to efficiently isolate immune complexes. The disclosed methods are rapid, highly specific and sensitive, require very small volumes of biological sample, and do not require the use of radioactivity. With these advantageous features, the disclosed methods are amendable for point-of-care testing (POCT), which is currently not available for the detection of autoantibodies associated with autoimmune disease or for the detection of many pathogen-specific antibodies.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: February 18, 2020
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventor: Peter D. Burbelo
  • Patent number: 10562024
    Abstract: The present disclosure relates to paper-based substrates and apparatus comprising such substrates. The apparatus may include a patterned conductive structure coupled to the paper-based substrate, wherein the patterned conductive structure responds to electromagnetic radiation.
    Type: Grant
    Filed: January 4, 2012
    Date of Patent: February 18, 2020
    Assignee: Tufts University
    Inventors: Fiorenzo Omenetto, David L. Kaplan, Hu Tao
  • Patent number: 10545142
    Abstract: The present invention relates to uniform nanostructure biosensors and methods of calibrating the response of nanostructure biosensors. The invention overcomes device to device variability that has made quantitative detection difficult. The described biosensors have uniform characteristics that allow for more reliable comparison across devices. The methods of the invention comprise normalizing the initial current rate, as measured by the nanostructure biosensor following the addition of an analyte, to device characteristics of the biosensor. The device characteristics of the biosensor which can be used to normalize the response include baseline current and transconductance, Calibration of responses allows for the generation of calibration curves for use in all devices to quantitatively detect an analyte, without the need for individual device calibration.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: January 28, 2020
    Assignee: Yale University
    Inventors: Tarek M. Fahmy, Aleksandar Vacic, Mark A. Reed
  • Patent number: 10539561
    Abstract: There is disclosed a process and an array for assaying for binding of target molecules to capture molecules on micro array devices, wherein the microarray devices contain electrodes. Specifically, there is disclosed a binding (including nucleotide hybridization) process to detect binding on a microarray wherein the microarray contains electronically addressable electrode devices. There is further disclosed an enzymatically catalyzed oxidation/reduction reaction to take place within a “virtual flask” region of a micro array wherein the reaction is detected by current changes detected on the addressable electrode.
    Type: Grant
    Filed: August 30, 2001
    Date of Patent: January 21, 2020
    Assignee: CustomArray, Inc.
    Inventor: Kilian Dill
  • Patent number: 10539580
    Abstract: Compositions and methods for detecting the presence and/or amount of one or more analytes, including analytes such as drugs of abuse, are provided. The compositions include two or more analytes associated with a solid phase, e.g., a particle or a multiwell plate. The compositions and methods also allow the simultaneous, tandem, or serial determination of the presence and/or amount of two or more analytes of interest in a sample.
    Type: Grant
    Filed: April 29, 2009
    Date of Patent: January 21, 2020
    Assignee: Psychemedics Corporation
    Inventors: Virginia Hill, Mohammad Atefi, Michael I. Schaffer
  • Patent number: 10539562
    Abstract: Provided is a testing device including: a porous flow path member in which a flow path for flowing a sample is formed; and a resin layer provided at at least one position over the flow path member, wherein the resin layer is a porous body formed of a hydrophobic material, and wherein a reagent reactive with the sample is formed as a solid phase over a surface of the resin layer facing the flow path member and inside voids of the resin layer.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: January 21, 2020
    Assignee: Ricoh Company, Ltd.
    Inventors: Rie Kobayashi, Naoki Shiraishi
  • Patent number: 10520514
    Abstract: According to a method for monitoring post-translational modifications of protein is provided, a first microbead by binding a protein antibody to a base bead is provided. A second microbead by binding a target protein having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first microbead, is provided. A third microbead by binding the second microbead to a first post-translational modification antibody is provided. A fourth microbead by binding the second microbead to a second post-translational modification antibody is provided. Impedances of the third and fourth microbeads are measured. A ratio of a first difference, between the impedances of the third microbead and a reference impedance, to a second difference, between the impedances of the fourth microbead and the reference impedance, is obtained.
    Type: Grant
    Filed: September 6, 2017
    Date of Patent: December 31, 2019
    Assignee: Korea Institute of Science and Technology
    Inventors: Soo-Hyun Lee, Ji-Yoon Kang, Min-Ho Kim, Yun-Kyung Kim
  • Patent number: 10520498
    Abstract: Seroprotection analysis systems, kits, and techniques are described for testing oral fluid for the presence of protective levels of antibodies of interest. In one aspect, the presence of protective levels of target antibodies indicates a test subject has achieved a target seroprotection level, such as but not limited to a target seroprotection level following vaccination. A collection kit can include a swab that enables collection of oral fluid containing antibodies from both dentulous and edentulous individuals, a container that can be pre-filled with an extraction solution, and a nozzle that can be coupled to the container to form a fluid-tight device. A user can use the nozzle to seal the swab in the container and to compress the swab within the container.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: December 31, 2019
    Assignees: Becton, Dickinson and Company, University of Maryland, Baltimore
    Inventors: Michael D. Fiechtner, Francis R. Go, Myron Levine, Scott Castanon
  • Patent number: 10514338
    Abstract: A target within a sample can be characterized using an energy source configured to transform a metal in the sample into a plasma and an optical spectroscopic detector configured to detect electromagnetic radiation emitted by the plasma to provide an optical-spectrum signal. A processor can determine presence of the metal in the sample using the optical-spectrum signal. The target can include a microbe or biological toxin. A recognition construct comprising a metal and a scaffold can be applied to the sample. The scaffold can bind to the target. Energy can be applied to transform at least some of the sample into a plasma. Electromagnetic radiation emitted by the plasma can be detected to provide an optical-spectrum signal of the sample. A preparation subsystem can add the recognition construct to the sample and a washing subsystem can wash unbound recognition construct out of the sample.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: December 24, 2019
    Assignee: Purdue Research Foundation
    Inventors: Joseph Paul Robinson, Bartlomiej P. Rajwa, Valery P. Patsekin, Euiwon Bae
  • Patent number: 10514379
    Abstract: The present invention relates to systems and methods that utilize a combination of immunoassay and magnetic immunoassay techniques to detect an analyte within an extended range of specified concentrations. In particular, a device includes a first immunosensor including an immobilized layer of capture antibodies configured to bind to a first complex of signal antibodies and cardiac troponin such that a second complex of the first complex and the immobilized layer of capture antibodies is localized on or near the first immunosensor. The device further includes a second immunosensor having a magnetic field disposed locally around the second immunosensor. The magnetic field is configured to attract magnetic beads such that a third complex of the first complex and capture antibodies immobilized on the magnetic beads is localized on or near the second immunosensor sensor.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: December 24, 2019
    Assignee: Abbott Point of Care Inc.
    Inventors: Jing Hua Hu, Antti Leo Oskari Virtanen, Eric Edward Potter, James T. K. Smith, Cary James Miller, John Lewis Emerson Campbell, Adam Roger Moss
  • Patent number: 10509006
    Abstract: Devices for use in determining properties of biochemicals and macromolecules derived from a biological sample include a fluid control unit and a macromolecule measurement unit integrated on a monolithic platform. Devices and methods of measuring the properties of macromolecules using immobilized magnetic particles are also disclosed.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: December 17, 2019
    Assignee: Axbio Inc.
    Inventors: Igor Ivanov, Albert Chueh, Licheng Niu, Hui Tian
  • Patent number: 10495636
    Abstract: A novel detection system and method is presented, where a two-bead receptor method is used for capturing pathogens, with one type of bead being magnetic and having a size of 3 microns or smaller, and the other type being polymeric and having a size of 3 microns or larger. The first type is used to concentrate a pathogen; the latter is used to create a detectable signal. Fast sensitive detection is achieved by collecting the optical signal created by the distortion of a homeotropically aligned chromonic azo dye in the presence of captured pathogens.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: December 3, 2019
    Assignee: Pathogen Systems, Inc.
    Inventor: Luana Tortora
  • Patent number: 10495634
    Abstract: The present invention provides a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a liquid sample by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD4+, CD8+ cells, malaria-infected red blood cells, cancer cells, cancer biomarkers such as prostate specific antigen and other cancer biomarkers, viruses, bacteria, and other pathogenic agents, with the sensitivity, specificity and accuracy of bench-top laboratory-based assays.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: December 3, 2019
    Assignee: Carnegie Mellon University
    Inventors: Alberto Gandini, James F. Antaki, Byron Wang Chuan, Joie N. Marhefka
  • Patent number: 10495571
    Abstract: A method for monitoring surface phenomena includes measuring a first surface plasmon resonance angle value (?SPR,REF) of a sample region (REG1), measuring a first critical angle value (?TIR,REF) of the sample region (REG1), causing a change of surface concentration (cM1,SRF) of an analyte (M1) at the sample region (REG1), changing the bulk composition at the sample region (REG1), measuring a second surface plasmon resonance angle value (?SPR(t)) of the sample region (REG1), measuring a second critical angle value (?TIR(t)) of the sample region (REG1), and determining an indicator value (?AUX(t)) indicative of the change of the surface concentration (cM1,SRF), wherein the indicator value (?AUX(t)) is determined from the second surface plasmon resonance angle value (?SPR(t)) by compensating an effect of the bulk composition, and wherein the magnitude (?COMP) of said effect is determined by using the second critical angle value (?TIR(t)).
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: December 3, 2019
    Assignee: BioNavis Oy
    Inventors: Janusz Sadowski, Niko Granqvist, Jussi Tuppurainen, Annika Jokinen
  • Patent number: 10495637
    Abstract: A universal sensor fabrication approach, molecular substrate imprinting technique, which utilizes the interaction between molecular building blocks and the surface of a transducer to develop specific molecular recognition cavities has been established. Integration of molecular recognition cavities with the surface of a nanoscale transducer will result in a nano-tunneling effect that takes place which will provide a sensor or a device that exhibits new properties not already exhibited by either the molecular recognition cavities on a bulk transducer or the nanotransducer material. One of the new properties of this nano-tunneling effect is that a universal potentiometric molecular sensor can be fabricated and used to detect any compounds, whether they are ions or molecules, with enhanced selectivity, sensitivity, and stability when molecular recognition cavities or elements are integrated on the surface of a nanoscale transducer.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: December 3, 2019
    Inventor: Yanxiu Zhou
  • Patent number: 10481154
    Abstract: Molecularly Imprinted Polymers (MIPs) are utilized to detect diseases and minimize false negative/positive scenarios. MIPs are implemented on a nano-electric circuit in a biochip where interactions of MIPs and an Antigen/Antibody (AG/AB) are detected, and disease specific biomarkers diagnosed. Biomarker detection is achieved with interdigitated gold electrodes in a biochip's microchannel. Capacitance changes due to biomarker interaction with AG/AB electrode coating diagnose diseases in a microfluidic environment. Biofluid passes through the microchannel and exposed to the nanocircuit to generate a capacitance difference and diagnose any specific disease in the biofluid sample. Blood capillary flow in a microchannel curved section experience centrifugal forces that separate liquid from solid. Various blood densities and segments experience different centrifugal effects while flowing through the curved section so serum is separated from various solid matter without using external devices.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: November 19, 2019
    Assignee: New Jersey Institute of Technology
    Inventors: Eon Soo Lee, Bharath Babu Nunna
  • Patent number: 10481164
    Abstract: The invention provides a method for controlling contaminants in biopharmaceutical purification processes by using light scattering and UV absorbance to establish a determinant. The invention makes use of multi-angle light scattering (MALS) and UV as a continuous monitoring system to provide information about the elution peak fractions in real-time instead of conventional pooling methods that rely on a predetermined percent UV peak max value to initiate the pooling process; regardless of product quality.
    Type: Grant
    Filed: March 26, 2013
    Date of Patent: November 19, 2019
    Assignee: AMGEN INC.
    Inventors: Arthur C. Hewig, III, Duke H. Phan, Yinges Yigzaw, Robert Bailey
  • Patent number: 10481002
    Abstract: A system for detecting an array of samples having detectable samples and at least one reference sample is provided. The system comprises an electromagnetic radiation source, a sensing surface comprising a plurality of sample fields, wherein the plurality of sample fields comprise at least one reference field, a phase difference generator configured to introduce differences in pathlengths of one or more samples in the array of samples, and an imaging spectrometer configured to image one or more samples in the array of samples.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: November 19, 2019
    Inventors: Masako Yamada, Sandip Maity, Sameer Dinkar Vartak, Rajesh Langoju, Abhijit Patil