Abstract: Provided are a recombinant hyaluronan and proteoglycan link protein 1 (HAPLN1), and a composition for preventing or treating pulmonary diseases, the composition comprising at least one selected from the group consisting of HAPLN1 (hyaluronan and proteoglycan link protein 1) protein, a gene coding for the HAPLN1 protein, and an effective agent for promoting the expression or activating the functions of HAPLN1 protein or gene as an active ingredient. The recombinant HAPLN1 according to the present disclosure may have superior effects of improving alveolar damage caused by aging or elastin reduction, and thus may effectively prevent or treat pulmonary diseases, such as chronic bronchitis, asthma, emphysema, and chronic obstructive pulmonary disease.

Abstract: An adenovirus expression vector is provided. The adenovirus expression vector may include: a) one or more mutations that render the adenovirus replication incompetent and b) at least one nucleotide sequence encoding a protein or an RNA is provided. A method of synthesizing an adenovirus vector is also provided. The synthesis may include: a) producing a plurality of overlapping adenovirus sub-fragments, each sub-fragment comprising a portion of the full genome of the adenovirus; b) circularizing the sub-fragments to form plasmid structures; and c) assembling the circularized sub-fragments into a linear structure, wherein the vector comprises a combination of two or more sub-fragments. A mammalian cell line configured to replicate adenoviral vectors, wherein the cell line comprises nucleotide sequences expressing E1A and E1B gene products but is devoid of other adenovirus sequences is also provided.