Abstract: Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.
Type:
Grant
Filed:
January 24, 2019
Date of Patent:
February 18, 2020
Assignee:
INNOVATIVE TARGETING SOLUTIONS INC.
Inventors:
Michael Gallo, Jaspal Singh Kang, Craig Robin Pigott