Abstract: Formulations of a cationic dye component (e.g., Azure A-Azure B-Azure C mixtures), an anionic dye component (e.g., Eosin Y) and an alcohol solvent (e.g., methanol or methanol-glycerol mixtures) are stabilized by an amino acid or its acid addition salt (e.g. lysine hydrochloride or glycine or a mixture of these).
Type:
Grant
Filed:
April 1, 1985
Date of Patent:
May 3, 1988
Assignee:
Fisher Scientific Company
Inventors:
Arjun Mallik, Daniel Koetters, Ludmilla Pluto
Abstract: A method for preparing biological specimens for the scanning electron microscope utilizes a select group of fluorocarbon solvents. Replacement of drying solvents with a fluorocarbon solvent, followed by direct sublimation, provides comparable results to critical point drying.