Patents Examined by Michele K. Joike
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Patent number: 9451770Abstract: A method of enhancing plant immunity is provided. The method comprises the step of administering to a plant a small molecule that binds to NPR1, or a functionally equivalent homolog thereof, that disrupts the interaction between N-terminal BTB/POZ domain and the C-terminal transactivation domain of NPR1. A method of screening for small molecule compounds that enhance plant immunity is also provided.Type: GrantFiled: June 7, 2013Date of Patent: September 27, 2016Inventor: Charles Despres
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Patent number: 9447457Abstract: The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization.Type: GrantFiled: April 9, 2010Date of Patent: September 20, 2016Assignee: SEEGENE, INC.Inventors: Jong Yoon Chun, In Taek Hwang
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Patent number: 9447451Abstract: Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.Type: GrantFiled: April 23, 2014Date of Patent: September 20, 2016Assignee: Cornell UniversityInventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway
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Patent number: 9447422Abstract: The present invention provides autonomous replication sequences (ARSs) isolated from Nannochloropsis that support the replication of episomal DNA molecules (EDMs) in eukaryotic cells. The ARSs and EDMs provided herein can be used for expressing genes in organisms including algae and heterokonts.Type: GrantFiled: December 6, 2013Date of Patent: September 20, 2016Assignee: Synthetic Genomics, Inc.Inventors: Peter DeHoff, Leah Soriaga, Srividya Akella
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Patent number: 9441205Abstract: Reverse engineering has offered new ways of studying the pathology of RNA viral infections, new more efficient devices of synthesizing recombinant viruses and developing vaccines and also demonstrated the versatility and efficiency of RNA dependent RNA polymerase RDRP system as an expression system. However, the currently used methods require a repertoire of complex, difficult-to-use tools. Present invention describes, a simpler plasmid based mammalian expression system that uses the RDRP enzyme activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses. This system will be useful for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.Type: GrantFiled: June 8, 2012Date of Patent: September 13, 2016Inventor: Vishwas Joshi
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Patent number: 9441211Abstract: The present invention relates to nucleic acid and amino acid sequences from Akkermansia muciniphila and from Bacteroides fragilis, coding for/representing novel alpha-1,3-fucosyltransferases. The invention also provides uses and methods for using the alpha-1,3-fucosyltransferases to generate fucosylated products, such as oligosaccharides, (glyco)proteins, or (glyco)lipids, in particular of 3-fucosyllactose.Type: GrantFiled: April 10, 2013Date of Patent: September 13, 2016Assignee: JENNEWEIN BIOTECHNOLOGIE GMBHInventors: Julia Parkot, Eric Hüfner, Stefan Jennewein
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Patent number: 9435055Abstract: The present invention is concerned with a method and a kit for detecting an interaction between a first membrane bound test protein or fragment thereof and a second test protein or fragment thereof which is either membrane bound or soluble with an in vivo genetic system based in yeast, bacterial or mammalian cells. The system makes use of the reconstitution of the split ubiquitin protein.Type: GrantFiled: March 28, 2003Date of Patent: September 6, 2016Assignee: Hybrigenics Services S.A.S.Inventors: Igor Stagljar, Michael Hottiger, Daniel Auerbach
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Patent number: 9416413Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.Type: GrantFiled: April 9, 2013Date of Patent: August 16, 2016Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan Schultz, John Davidson
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Patent number: 9410158Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: September 24, 2013Date of Patent: August 9, 2016Assignee: Cargill IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Patent number: 9409960Abstract: The present invention provides a nucleic acid molecule which encodes a polypeptide consisting of two methionine residues as the first and second N-terminal amino acid residues linked via a peptide bond to a mature eukaryotic histone. The present invention furthermore relates to a vector containing said nucleic acid molecule, a host transformed with said vector, polypeptides encoded by the nucleic acid molecule and pharmaceutical and diagnostic compositions. The present invention also relates to the use of the nucleic acid molecule, vectors, hosts and the polypeptide of the invention for the preparation of a composition for the treatment of diseases. Furthermore, the present invention relates to a method of testing for the presence of the nucleic acid molecule or the polypeptide in a sample and to a kit.Type: GrantFiled: April 7, 2008Date of Patent: August 9, 2016Assignee: Symbiotec Gesellschaft zur Forschung und Entwicklung auf dem Gebiet der Biotechnologie mbHInventors: Peter Gross, Hans Jornvall, Grazyna Formicka-Zeppezauer, Michael Zeppezauer, Michel Thiry
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Patent number: 9410176Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.Type: GrantFiled: December 14, 2011Date of Patent: August 9, 2016Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTD.Inventors: Megumi Kurokawa, Yoko Hayashi, Masayoshi Tsukahara
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Patent number: 9404157Abstract: Disclosed is a method to achieve digital quantification of DNA (i.e., counting differences between identical sequences) using direct shotgun sequencing followed by mapping to the chromosome of origin and enumeration of fragments per chromosome. The preferred method uses massively parallel sequencing, which can produce tens of millions of short sequence tags in a single run and enabling a sampling that can be statistically evaluated. By counting the number of sequence tags mapped to a predefined window in each chromosome, the over- or under-representation of any chromosome in maternal plasma DNA contributed by an aneuploid fetus can be detected. This method does not require the differentiation of fetal versus maternal DNA. The median count of autosomal values is used as a normalization constant to account for differences in total number of sequence tags is used for comparison between samples and between chromosomes.Type: GrantFiled: June 19, 2013Date of Patent: August 2, 2016Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Hei-Mun Christina Fan, Stephen R. Quake
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Patent number: 9388405Abstract: The present invention generally relates to subpopulations of mammalian cells with distinctive ribosome translational profiles, i.e. translational activities. The present invention further relates to methods for identifying and isolating such cells, kits comprising the same, or methods which utilize different translational activities of these subpopulations of mammalian cells.Type: GrantFiled: February 18, 2010Date of Patent: July 12, 2016Assignee: Wayne State UniversityInventors: Leon Carlock, Maria Cypher
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Patent number: 9389223Abstract: The invention provides methods for quickly and easily screening mixed cell samples for a pharmacodynamic effect to a drug or test agent.Type: GrantFiled: August 24, 2006Date of Patent: July 12, 2016Assignee: The United States of America, as represented by the Secretary, Department of Health & Human ServicesInventors: Jane Trepel, Eun Joo Chung
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Patent number: 9388407Abstract: Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.Type: GrantFiled: March 31, 2014Date of Patent: July 12, 2016Assignee: Gen9, Inc.Inventor: Joseph Jacobson
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Patent number: 9388453Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.Type: GrantFiled: November 21, 2014Date of Patent: July 12, 2016Assignee: GENEWEAVE BIOSCIENCES, INC.Inventor: Diego Ariel Rey
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Patent number: 9382330Abstract: Methods of diagnosing cancer that are based in part on the findings that Pdia4 promotes cell growth, cell proliferation, and cell cycle are disclosed herein. Methods of using microvessel density as a surrogate marker and reducing tumor microvessel density in a subject are also enclosed herein.Type: GrantFiled: May 9, 2011Date of Patent: July 5, 2016Assignee: ACADEMIA SINICAInventor: Wen-Ching Yang
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Patent number: 9371569Abstract: The present invention relates to methods and kits for identifying, diagnosing, prognosing, and monitoring cervical cancer. These methods include determining the methylation status or the expression levels of particular genes, or a combination thereof.Type: GrantFiled: February 13, 2014Date of Patent: June 21, 2016Assignee: MDxHealth S.A.Inventors: Wim Van Criekinge, Valerie Deregowski, Luc Dehaspe, G. Bea A. Wisman, Ate G. J. Van der Zee, E. M. D. Schuuring
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Patent number: 9370551Abstract: The present invention provides methods related to the treatment of head and neck squamous cell carcinoma (HNSCC) and its associated premalignant lesions. In particular, the invention features methods which may specifically target HNSCC-associated genes and alter gene expression to treat or alleviate a symptom of HNSCC, or its related premalignant lesions. These methods may involve decreasing the function of an HNSCC-associated gene with aberrant gain-of-function; or increasing the function of an HNSCC-associated gene with aberrant loss-of-function.Type: GrantFiled: July 25, 2012Date of Patent: June 21, 2016Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College, Massachusetts Eye and Ear Infirmary, Dana-Farber Cancer Institute, Inc., University of Pittsburgh-Of The Commonwealth of Higher Education, Instituto Carlos Slim de la Salud, A.C.Inventors: Le Cong, Ann Marie Egloff, Levi A. Garraway, Jennifer Rubin Grandis, Eric S. Lander, Nicholas Stransky, Aaron D. Tward, Feng Zhang
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Patent number: 9365867Abstract: Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.Type: GrantFiled: March 12, 2013Date of Patent: June 14, 2016Assignee: The Catholic University of AmericaInventor: Venigalla B. Rao