Abstract: Devices, kits, and methods related to embodiments of an improved liquid test sample injection device comprising a sample mixture that comprises at least one sample flag compound for detecting the presence or non-presence of a patient's liquid test sample upon being interrogated by a pre-determined wavelength of light.

Abstract: A pipette for use with a pipette tip or syringe including a piston positioned within a cylinder comprises a pipette housing extending between an upper end and a lower end. Two parallel rods are positioned in the pipette housing, wherein one of the two parallel rods is coupled to a lifting rod. An operating element is configured to protrude outwardly from the pipette housing and be displaced relative to the pipette housing. A gear mechanism is arranged in the pipette housing and comprises a drive comprising a moveable input member and configured to be driven by the operating element, and an output comprising at least one moveable output member and configured to drive the two parallel rods. Successive displacements of the input member by the operating element are configured to alternately displace one of the two parallel rods and then another of the two parallel rods by the output member.

Abstract: A fluid handling device includes a sample channel configured to carry a sample; a dispersion medium channel configured to carry dispersion medium; a dispersion liquid generation part connected to the sample channel and the dispersion medium channel, and configured to generate dispersion liquid by dividing the sample by the dispersion medium, the dispersion liquid being liquid in which droplets of the sample are dispersed in the dispersion medium; and a dispersion liquid channel connected to the dispersion liquid generation part. The dispersion liquid generation part includes a protrusion.

Abstract: The microfluidic board comprises a plurality of matrix units, wherein each matrix unit is a stacked arrangement comprising a driving portion comprising an actuator, a pump portion in contact with the driving portion and comprising a pump, a channel portion in contact with the pump portion and comprising one or more channels, and a chamber portion in contact with the channel portion and comprising a chamber, wherein the one or more channels are configured to direct fluid between the pump and the chamber, and wherein the actuator is configured to generate a force to drive the pump upon receiving of an input energy.

Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

Abstract: A modular fluidic chip includes a body configured to have at least one flow channel formed in an inside thereof and be connected to another modular fluidic chip to allow the at least one flow channel to communicate with a flow channel provided in the other modular fluidic chip. A fluidic chip capable of performing one function is formed in the form of a module, whereby a fluidic flow system of various structures can be implemented without restriction in shape or size by connecting a plurality of fluidic chips capable of performing different functions as necessary. Through this, various and accurate experimental data can be obtained, and when a specific portion is deformed or damaged, only the fluidic chip corresponding thereto can be replaced, thereby reducing manufacture and maintenance costs.

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: The present disclosure relates to a ferrocene-based compound having high selectivity for sulfide ion. The ferrocene-based compound is obtained by reacting a ferrocene starting material with one or more organic compound selected from a nitrile, a methyl ester and an ethyl ester, and a chemical sensor in solution state is prepared by mixing the compound with an organic solvent. The chemical sensor according to the present disclosure has high selectivity and sensitivity for sulfide ion even at low concentration and may be used as a chemical sensor for detecting hydrogen sulfide in solution state by allowing visual inspection of sulfide ion.

Abstract: This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.

Abstract: The present invention relates to a method for quantitative measurement of catechol estrogen bound protein in blood sample. By detecting adduction levels of binding sites of the catechol estrogen on the protein in blood sample, the catechol estrogen bound protein in the blood sample can be detected quantitatively and a limit of quantitation can be decreased.

Abstract: The present disclosure provides a test strip for milk immunofluorescence assay (IFA) and use thereof, and relates to the technical field of test strip. The test strip of the present disclosure includes a sample pad, a conjugate pad, a nitrocellulose membrane, and a wicking pad assembled and pasted successively on a PVC backing card; fluorescent latex microsphere-labeled mixed antibodies are coated on the conjugate pad; anti-casein antibody (T1 line), anti-beta-lactoglobulin (BLG) antibody (T2 line), anti-alpha-lactalbumin (ALA) antibody (T3 line), anti-lactoferrin/anti-bovine serum albumin (BSA) antibody (T4 line), and rabbit anti-mouse IgG antibody (C line) are coated on the nitrocellulose membrane, where the T1, T2, T3, and T4 lines are test lines, and the C line is a control line. The test strip of the present disclosure accurately and quantitatively detects the content of casein, BLG, ALA, and lactoferrin/BSA in food, and features easy operation and high accuracy and sensitivity.

Abstract: The present invention discloses a pencil-like optical fiber sensor probe, including an inner tube, a light screening casing, a clamping device, an optical fiber and an optical probe; a portable immunosensor, including the pencil-like optical fiber sensor probe, an immersion immune response reagent strip, a touch-screen computer, a compact battery-powered sensitive photon counting detector and a case; and a use of the immunosens in detecting inflammatory markers. The design of the pencil-like optical sensor probe greatly simplifies the immune analysis process by combining the immersion immune response reagent strip. Each optical probe allows for up to 10 immunoassays, which reduces the experimental cost and avoids frequent replacement of the probe. The integrated detecting system is powered by battery which is suitable for in-situ analysis and detection. The sensor also has a high stability and sensitivity.

Abstract: A modular fluid chip according to an embodiment of the present disclosure includes a body including at least one first hole which allows fluid to flow therethrough; and a housing receiving the body therein, and including a second hole which corresponds to the at least one first hole and allows the fluid to flow therethrough, and a fluid connection part which is connectable to another modular fluid chip.

Abstract: The present invention provides a chemical sensor comprising a substrate, a first colorimetric sensor array exposed and arranged in a first accommodating space of the substrate and a second colorimetric sensor array arranged in a second accommodating space of the substrate. The second accommodating space is covered with an isolating layer to isolates liquid molecules but allows gas molecules to pass through. The first colorimetric sensor array changes from a first initial color to a first indicating color according to a volatile part and a non-volatile part of an analyte, and the second colorimetric sensor array changes from a second initial color to a second indicating color according to the volatile part of the analyte, so that information of the volatile part and the non-volatile part of the analyte can be obtained simultaneously.

Abstract: A microfluidic device for processing a liquid in ludci includes at least one pneumatic substrate with a pneumatic cavity and a fluidic substrate with a fluidic cavity for accommodating the liquid. The fluidic cavity is arranged opposite the pneumatic cavity. In addition, the microfluidic device has a flexible membrane which is arranged between the pneumatic substrate and the fluidic substrate. The flexible membrane is designed to fluidically separate, from one another, a fluidic chamber extending at least in part in the fluidic cavity and a pneumatic chamber extending at least in part in the pneumatic cavity. The microfluidic device further includes a first pneumatic channel for applying a first pneumatic pressure to the pneumatic chamber and a second pneumatic channel for applying a second pneumatic pressure to the pneumatic chamber.

Abstract: A melting device is provided that melts a bio-derived frozen product contained in a container including a heat transfer section comprising at least two heating bags, each of which is filled with a heating liquid and is capable of sandwiching the container between the at least two heating bags and a suction mechanism that sucks air from a space between the at least two heating bags and surrounding the container.