Patents Examined by Miguel H. Escallon
  • Patent number: 5429923
    Abstract: A method is described for diagnosing individuals as having hypertrophic cardiomyopathy, e.g. familial or sporadic hypertrophic cardiomyopathy. The method provides a useful diagnostic tool which becomes particularly important when testing asymptomatic individuals suspected of having the disease. Symptomatic individuals have a much better chance of being diagnosed properly by a physician. Asymptomatic individuals from families having a history of familial hypertrophic cardiomyopathy may be selectively screened using the method of this invention allowing for a diagnosis prior to the appearance of any symptoms. Individuals having the mutation responsible for the disease may be counseled to take steps which hopefully would prolong their life, i.e. avoid rigorous exercise. The methodology used in the above method also has broad applicability and may be used to detect other disease-associated mutations in DNA obtained from subjects being tested for other disease-associated mutations.
    Type: Grant
    Filed: December 11, 1992
    Date of Patent: July 4, 1995
    Assignees: President and Fellows of Harvard College, Brigham and Women's Hospital, The General Hospital
    Inventors: Christine Seidman, John Seidman, Hugh Watkins, Anthony Rosenzweig
  • Patent number: 5378604
    Abstract: This invention relates to compositions of oligonucleotide probes for use in the detection of bacteria associated with medical disorders of the human mouth, wherein said probes consist essentially of a segment of nucleic acid capable of selectively hybridizing under hybridizing conditions, to the 16S or 23S ribosomal RNA [rRNA] of said bacteria. Methods for detection, as well as diagnostic kits for the assay of these bacterium, are also disclosed.
    Type: Grant
    Filed: January 12, 1993
    Date of Patent: January 3, 1995
    Assignee: MicroProbe Corporation
    Inventors: Dennis E. Schwartz, Roy H. Kanemoto, Susan M. Watanabe, Kim Dix
  • Patent number: 5310652
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
    Type: Grant
    Filed: June 24, 1993
    Date of Patent: May 10, 1994
    Assignee: Hoffman-La Roche Inc.
    Inventors: David H. Gelfand, Thomas W. Myers
  • Patent number: 5306625
    Abstract: A microbiological process for terminal hydroxylation of ethyl groups on aromatic 5- or 6-member ring heterocycles. The hydroxylation is performed with microorganisms which:(a) contain the genes of a Pseudomonas OCT plasmid which form an active alkane monooxygenase, and(b) form no active chromosomal or plasmid-coded alcohol dehydrogenase.
    Type: Grant
    Filed: August 3, 1993
    Date of Patent: April 26, 1994
    Assignee: Lonza Ltd.
    Inventors: Andreas Kiener, Thomas Zimmermann
  • Patent number: 5304469
    Abstract: The present invention provides an improved method and reagent for measuring the total carbon dioxide content in a sample of body fluid. The improvement comprises adding a sufficient amount of propylene glycol to the PEPC enzyme reagent to increase the activity of PEPC and maintaining the enzyme reagent at a substantially constant pH of about 6.0 to 7.0.
    Type: Grant
    Filed: October 17, 1989
    Date of Patent: April 19, 1994
    Assignee: Abbott Laboratories
    Inventor: Charlie W. Wilson, III
  • Patent number: 5300428
    Abstract: A detection system for anaerobic micro-organisms involves the addition to a nutrient medium for the micro-organisms of an indicator species which is electrochemically reducible, soluble in the nutrient medium and consumed by the micro-organisms during growth. Two electrodes are immersed in the nutrient medium and changes in an electrical property of the nutrient medium are monitored. A preferred indicator species is riboflavin.
    Type: Grant
    Filed: June 29, 1992
    Date of Patent: April 5, 1994
    Assignee: CMB Packaging (UK) Limited
    Inventors: Martin R. Ackland, John K. Blundell, William M. Hedges, James F. Walpole
  • Patent number: 5283175
    Abstract: The subject invention provides DNA oligomers complementary to portions of the flagellin gene of Borrelia burgdorferi which can be used to detect the organism, as well as other Borrelia species, in a sample. PCR technology can be used to amplify the portion of the flagellin gene which is then detected using a biotinylated probe provided by the subject invention.
    Type: Grant
    Filed: April 15, 1991
    Date of Patent: February 1, 1994
    Assignee: The Research Foundation of State University of New York
    Inventors: Terry L. Weaver, Darla J. Wise
  • Patent number: 5196303
    Abstract: A cloned single-strand DNA encoding amino acid sequence for a eukaryotic transcription factor S-II is disclosed together with, a cloned double-strand DNA consisting of the single-strand DNA and its complementary single-strand DNA, a DNA fragment of the single- or double-strand DNA, a process for the preparation thereof, a plasmid, in which the double-strand DNA or its fragment is inserted, and a diagnosis of viral diseases and cancers.
    Type: Grant
    Filed: April 11, 1991
    Date of Patent: March 23, 1993
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventor: Shunji Natori
  • Patent number: 5149628
    Abstract: Bcl-3 gene sequences can be used to monitor the success of chemotherapy and to detect minimal residual disease in patients having hematopoietic malignancies. Bcl-3 sequences can also be used to identify new cellular oncogenes involved in hematopoietic cell malignancies by taking advantage of their adjacency to the bcl-3 promoter. Particular nucleic acid probes and primers are also provided by the disclosure.
    Type: Grant
    Filed: November 15, 1989
    Date of Patent: September 22, 1992
    Assignee: Temple University
    Inventor: Carlo M. Croce
  • Patent number: 5073483
    Abstract: A method of staining a nucleic acid sequence which is fixed on a solid support is described wherein an enzymatic system which fixes this sequence is employed to detect it by non-radioactive labelling, the enzymatic system being subsequently reacted with chromogenic substrate to produce a colored product. A developer solution is used comprising a sulfur organic compound constituting the substrate which is adapted to be hydrolized by the enzyme to form an organic thiol, and a metallic compound, for example a gold compound. The metallic compound is soluble and stable in aqueous solution and can react with the organic thiol to form a metal-organic compound complex having a characteristic color which precipitates in situ, which metal-organic complex subsequently may be decolorized with a reducing agent to thus allow reuse of the solid support. A set, or kit, of reactants for carrying out the method also is disclosed.
    Type: Grant
    Filed: March 24, 1989
    Date of Patent: December 17, 1991
    Assignee: Bioprobe Systems
    Inventor: Philippe Lebacq
  • Patent number: 5021335
    Abstract: In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with reverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
    Type: Grant
    Filed: June 17, 1988
    Date of Patent: June 4, 1991
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Laurence Tecott, Jack D. Barchas, James Eberwine