Patents Examined by Mohammad Y. Meah
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Patent number: 12037613Abstract: The present invention relates to phytase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: August 13, 2021Date of Patent: July 16, 2024Assignee: Novozymes A/SInventors: Hengxiao Zhai, Jesper Vind, Lars Kobberoe Skov, Qian Zhang, Ester Santigosa, Jose-Otavio B. Sorbara, Aurelia Anne Catherine Charlotte Seon, Carrie Louise Walk
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Patent number: 12024710Abstract: A recombinant microorganism of the genus Escherichia, comprises a genetic modification that increases expression of a nosZ gene encoding NosZ, which is a nitrous oxide reductase, in the recombinant microorganism, wherein the recombinant microorganism comprises a nosR gene encoding NosR, a nosD gene encoding NosD, a nosF gene encoding NosF, a nosY gene encoding NosY, and an apbE gene encoding ApbE, and wherein the nosR gene, the nosD gene, the nosF gene, the nosY gene and the apbE gene are derived from a microorganism of the genus Pseudomonas, the genus Paracoccus, or a combination thereof.Type: GrantFiled: May 26, 2021Date of Patent: July 2, 2024Assignee: SAMSUNG ELECTRONICS CO., LTD.Inventors: Woo Yong Shim, Seung Hoon Song, Jae-Young Kim, Dongsik Yang, Yu Kyung Jung
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Patent number: 12024731Abstract: The present disclosure provides methods for preparing ?-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a ?-substituted serine, and iii) a tryptophan synthase ?-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the ?-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new ?-substituted tryptophan analogs are also described.Type: GrantFiled: December 20, 2021Date of Patent: July 2, 2024Assignee: California Institute of TechnologyInventors: Christina E. Boville, Sabine Brinkmann-Chen, Andrew R. Buller, David K. Romney, Christopher K. Prier, Philipp Koch, Remkes A. Scheele
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Patent number: 12018326Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: GrantFiled: October 26, 2021Date of Patent: June 25, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Patent number: 11987821Abstract: Disclosed herein are compositions and methods of treating disclosure provides for compounds for use in treating Mitochondrial Neurogastrointestinal Encephalopathy Syndrome (MNGIE). In some embodiments, the compounds have cell penetrating activity and thymidine phosphorylase activity. In certain embodiments, the compounds disclosed herein comprise: a) at least one cell-penetrating peptide (CPP) moiety; and b) a thymidine phosphorylase, or an active fragment or analog thereof (TP), wherein the CPP is coupled, directly or indirectly, to TP.Type: GrantFiled: October 6, 2021Date of Patent: May 21, 2024Assignee: Entrada Therapeutics, Inc.Inventors: Natarajan Sethuraman, Jason Ruth, Louis A. Tartaglia, Dehua Pei, Ziqing Qian
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Patent number: 11987774Abstract: Processes and systems for recovering products from a corn fermentation mash. In one example, a process for recovering products from a corn fermentation mash can include separating ethanol from a fermentation mash to produce a whole stillage. The fermentation mash can be derived from a ground corn product milled from a plurality of corn pieces. The plurality of corn pieces can include whole corn kernels, fragmented corn kernels, size-reduced corn kernels, milled corn kernels, or a mixture thereof. Greater than 25 wt % of the ground corn product can have a particle size of greater than 105 ?m and greater than 80 wt % of the ground corn product can have a particle size of 425 ?m or less, as measured according to AOAC 965.22-1966. The process can also include separating the whole stillage to produce a fiber rich portion and a filtrate.Type: GrantFiled: February 7, 2022Date of Patent: May 21, 2024Assignee: POET GRAIN (OCTANE), LLCInventor: Jason Bootsma
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Patent number: 11976312Abstract: Provided is a method for preparing Rebaudioside C using an enzymatic method, comprising using rubusoside or dulcoside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside C.Type: GrantFiled: March 30, 2022Date of Patent: May 7, 2024Assignee: PepsiCo, Inc.Inventors: Alex Tao, Guoqing Li, Wenxia Wang, Leilei Zheng, Chunlei Zhu, Xiaoliang Liang, Kuikiu Chan
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Patent number: 11976313Abstract: Provided is a method for preparing rebaudioside N using an enzymatic method, comprising using rebaudioside A or rebaudioside J as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyl-transferase and/or a UDP-glycosyltransferase-containing recombinant cell to generate rebaudioside N.Type: GrantFiled: May 16, 2022Date of Patent: May 7, 2024Assignee: PepsiCo, Inc.Inventors: Alex Tao, Guoqing Li, Wenxia Wang, Leilei Zheng, Chunlei Zhu, Xiaoliang Liang, Kuikiu Chan
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Patent number: 11965197Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.Type: GrantFiled: March 10, 2021Date of Patent: April 23, 2024Assignee: Rensselaer Polytechnic InstituteInventors: Mattheos Koffas, John Andrew Jones
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Patent number: 11965167Abstract: This document relates to materials and methods for the production of protein. In one aspect, this document provides a first exogenous nucleic acid construct including a nucleotide sequence encoding an aminolevulinate synthase (ALAS) protein operably linked to a first promoter element, wherein the ALAS includes at least a first heme responsive motif (HRM), and wherein the ALAS includes a mutation in the first HRM, and a second exogenous nucleic acid construct comprising a nucleotide sequence encoding a heme-binding protein, wherein the second exogenous nucleic acid construct including a nucleotide sequence encoding the heme-binding protein is operably linked to the first promoter element or is operably linked to a second promoter element.Type: GrantFiled: April 24, 2020Date of Patent: April 23, 2024Assignee: Impossible Foods Inc.Inventors: Biswajoy Roy Chaudhuri, Smita Shankar
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Patent number: 11959092Abstract: The present invention discloses a process for engineering a host cell comprising the steps of; a) integrating a first polynucleotide cassette including a first selection marker flanked by a first pair of recombination sites; b) removing the first selection marker by the action of a recombinase which recognises the first pair of recombination sites; c) integrating a second polynucleotide cassette including a second selection marker flanked by a second pair of recombination sites; and d) removing the second selection marker by the action of a recombinase which recognises the second pair of recombination sites. Also disclosed is a host cell genome polynucleotide comprising a first recombinantly engineered region and a second recombinantly engineered region, wherein a first single recombination site is adjacent to the first recombinantly engineered region, and a second single recombination site is adjacent to the second recombinantly engineered region.Type: GrantFiled: August 7, 2018Date of Patent: April 16, 2024Assignee: GLAXOSMITHKLINE BIOLOGICALS SAInventors: Amirreza Faridmoayer, Michael Thomas Kowarik, Gerd Martin Lipowsky, Fabio Serventi
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Patent number: 11952575Abstract: A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.Type: GrantFiled: November 15, 2017Date of Patent: April 9, 2024Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTDInventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Xin Huang, Yulei Ma, Yibing Cheng
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Patent number: 11946086Abstract: This invention is intended to identify a gene cluster involved in biosynthesis of a cyclic peptide compound produced by a filamentous fungus of the Curvularia species and to establish a system for synthesizing such cyclic peptide compound. The gene is composed of a first module to a tenth module and encodes a protein having activity of synthesizing a nonribosomal peptide constituting a basic peptide backbone of a cyclic peptide compound produced by a filamentous fungus of the Curvularia species.Type: GrantFiled: December 13, 2021Date of Patent: April 2, 2024Assignees: TOHOKU UNIVERSITY, KUMIAI CHEMICAL INDUSTRY CO., LTD.Inventors: Takashi Kubo, Masayuki Machida, Maiko Umemura, Keietsu Abe, Akira Yoshimi, Tomonori Fujioka, Shigenari Yamaguchi, Kiyoshi Kawai
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Patent number: 11939599Abstract: Disclosed are a gene mining method combining functional sequence and structure simulation, an NADH-preferring phosphinothricin dehydrogenase mutant and an application thereof. The gene mining method comprises the following steps: (1) analyzing a characteristic sequence which an NADH-type glutamate dehydrogenase should have; (2) searching a gene library based on the characteristic sequence; (3) performing clustering analysis and protein structure simulation on genes obtained by the searching; (4) selecting genes that feature high gene aggregation and a protein structure similar to that of the known phosphinothricin dehydrogenase as candidate genes. A wild-type phosphinothricin dehydrogenase with an amino acid sequence as set forth in SEQ ID No.Type: GrantFiled: December 30, 2021Date of Patent: March 26, 2024Assignee: ZHEJIANG UNIVERSITY OF TECHNOLOGYInventors: Yaping Xue, Feng Cheng, Jiamin Zhang, Shuping Zou, Jianmiao Xu, Yuguo Zheng
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Patent number: 11932881Abstract: A heparin skeleton synthase originates from Neisseria animaloris, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA from Escherichia coli K5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.Type: GrantFiled: January 3, 2022Date of Patent: March 19, 2024Assignees: SHAN DONG UNIVERSITY, Bloomage Biotechnology Corporation LimitedInventors: Juzheng Sheng, Xueping Guo, Jianqun Deng, Fengshan Wang, Zhen Lu, Ranran Du, Liu Sun, Yuanjun Sun
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Patent number: 11932893Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.Type: GrantFiled: May 4, 2018Date of Patent: March 19, 2024Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Robin E. Osterhout
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Patent number: 11926850Abstract: The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume glucose and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).Type: GrantFiled: March 17, 2021Date of Patent: March 12, 2024Assignee: CARGILL, INCORPORATEDInventors: Arlene M. Fosmer, Christopher K. Miller, Gregory Michael Poynter, Brian Jeffrey Rush, Jon Veldhouse
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Patent number: 11920167Abstract: The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with ?-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.Type: GrantFiled: October 27, 2020Date of Patent: March 5, 2024Assignee: TATE & LYLE SOLUTIONS USA LLCInventors: Jonathan Vroom, Stephanie Sue Galanie, Nikki Dellas, Jack Liang, Joyce Liu, David Entwistle, Courtney Dianne Moffett
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Patent number: 11881606Abstract: “Biocathode MCL,” designated for its main bacterial constituents (Marinobacter, Chromatiaceae, and Labrenzia), is a stable microbial community enriched from seawater that forms biofilms on the surfaces of electrodes. These biofilms are effective to perform carbon fixation without the need for external electrical power nor sunlight applied thereto.Type: GrantFiled: December 9, 2020Date of Patent: January 23, 2024Assignee: The Government of the United States of America, as repersented by the Secretary of the NavyInventors: Sarah M. Glaven, Lina Bird, Leonard M. Tender
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Patent number: 11859189Abstract: The present disclosure provides host cells, e.g., bacterial cells, that comprise a methionine decarboxylase enzyme for the treatment of diseases and disorders associated with methionine metabolism in a subject. The disclosure further provides pharmaceutical compositions and methods of treating disorders associated with methionine metabolism, such as homocystinuria.Type: GrantFiled: August 11, 2022Date of Patent: January 2, 2024Assignees: Synlogic Operating Company, Inc., Ginkgo Bioworks, Inc.Inventors: Dylan Alexander Carlin, Vincent M. Isabella, Jonathan McMurry, Theodore Carlton Moore, III, Mylene Perreault, Nathan Schmidt, Mark Simon