Patents Examined by Mohammad Y. Meah
  • Patent number: 11881606
    Abstract: “Biocathode MCL,” designated for its main bacterial constituents (Marinobacter, Chromatiaceae, and Labrenzia), is a stable microbial community enriched from seawater that forms biofilms on the surfaces of electrodes. These biofilms are effective to perform carbon fixation without the need for external electrical power nor sunlight applied thereto.
    Type: Grant
    Filed: December 9, 2020
    Date of Patent: January 23, 2024
    Assignee: The Government of the United States of America, as repersented by the Secretary of the Navy
    Inventors: Sarah M. Glaven, Lina Bird, Leonard M. Tender
  • Patent number: 11859189
    Abstract: The present disclosure provides host cells, e.g., bacterial cells, that comprise a methionine decarboxylase enzyme for the treatment of diseases and disorders associated with methionine metabolism in a subject. The disclosure further provides pharmaceutical compositions and methods of treating disorders associated with methionine metabolism, such as homocystinuria.
    Type: Grant
    Filed: August 11, 2022
    Date of Patent: January 2, 2024
    Assignees: Synlogic Operating Company, Inc., Ginkgo Bioworks, Inc.
    Inventors: Dylan Alexander Carlin, Vincent M. Isabella, Jonathan McMurry, Theodore Carlton Moore, III, Mylene Perreault, Nathan Schmidt, Mark Simon
  • Patent number: 11859225
    Abstract: A method of epimerizing an (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid is provided. The method comprises contacting the (S)-1-benzylisoquinoline alkaloid with at least one enzyme. Contacting the (S)-1-benzylisoquinoline alkaloid with the at least one enzyme converts the (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: January 2, 2024
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Christina D. Smolke, Stephanie Galanie, Isis Trenchard, Catherine Thodey, Yanran Li
  • Patent number: 11802299
    Abstract: An enzyme-catalyzed method of synthesizing (2S,3R)-2-substituted aminomethyl-3-hydroxybutyrate, including: preparing engineered bacteria containing a carbonyl reductase SsCR-encoding gene; preparing a resting cell suspension of the engineered bacteria; preparing a culture containing carbonyl reductase; and mixing the culture containing carbonyl reductase with substrate 2-substituted aminomethyl-3-one butyrate, glucose dehydrogenase, a cosolvent, glucose and a cofactor followed by asymmetric carbonyl reduction to obtain (2S,3R)-2-substituted aminomethyl-3-hydroxybutyrate. The amino acid sequence of the carbonyl reductase is shown in SEQ ID NO.1.
    Type: Grant
    Filed: December 8, 2021
    Date of Patent: October 31, 2023
    Assignee: Fudan University
    Inventors: Fener Chen, Yuan Tao, Zedu Huang, Dang Cheng, Ge Meng
  • Patent number: 11795486
    Abstract: The present invention relates to an enzyme-catalyzed process for producing GDP-fucose from low-cost substrates guanosine and L-fucose or guanosine and D-Mannose in a single reaction mixture. Said process can be operated (semi)continuously or in batch mode. Further, said process can be adapted to produce fucosylated molecules and biomolecules including glycans, such as human milk oligosaccharides, proteins, peptides, glycoproteins or glycopeptides.
    Type: Grant
    Filed: March 31, 2020
    Date of Patent: October 24, 2023
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
    Inventors: Reza Mahour, Thomas F. T. Rexer
  • Patent number: 11780905
    Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.
    Type: Grant
    Filed: February 8, 2021
    Date of Patent: October 10, 2023
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Fei Xu, Jinyuan Hu
  • Patent number: 11773424
    Abstract: A recombinant host cell comprising an endogenous gene encoding a FLO8 protein comprising the amino acid sequence identified as SEQ ID NO:1 or a homologue thereof, which host cell is engineered by one or more genetic modifications to reduce expression of said gene compared to the host cell prior to said one or more genetic modifications, and which host cell comprises a heterologous expression cassette comprising a gene of interest (GO!) under the control of an expression cassette promoter (ECP) which ECP is repressible by a non-methanol carbon source, and a method of producing a protein of interest using said recombinant host cell.
    Type: Grant
    Filed: January 10, 2020
    Date of Patent: October 3, 2023
    Assignee: LONZA LTD
    Inventors: Brigitte Gasser, Corinna Rebnegger, Mirelle Citiali Flores Villegas, Diethard Mattanovich
  • Patent number: 11746364
    Abstract: Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
    Type: Grant
    Filed: March 3, 2021
    Date of Patent: September 5, 2023
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Tomás Pluskal, Jing-Ke Weng
  • Patent number: 11739354
    Abstract: Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
    Type: Grant
    Filed: March 3, 2021
    Date of Patent: August 29, 2023
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Tomás Pluskal, Jing-Ke Weng
  • Patent number: 11713451
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: October 29, 2020
    Date of Patent: August 1, 2023
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 11713454
    Abstract: The present invention provides a mutated histidine decarboxylase suitable for a practical use. Specifically, the present invention provides a mutated histidine decarboxylase having at least one amino acid residue mutated as compared to a wild-type histidine decarboxylase, and having higher histidine decarboxylase activity and/or stability than the wild-type histidine decarboxylase, and also a use thereof. The mutated histidine decarboxylase has Motifs (1) to (6), and an amino acid residue in at least one motif thereof can be mutated. The mutated histidine decarboxylase can also have a mutation of at least one amino acid residue in an amino acid sequence designated by SEQ ID NO: 3 and in a homologous sequence thereto.
    Type: Grant
    Filed: September 10, 2019
    Date of Patent: August 1, 2023
    Assignee: AJINOMOTO CO., INC.
    Inventors: Hiroki Yamaguchi, Masayuki Sugiki, Kunio Nakata
  • Patent number: 11708389
    Abstract: Disclosed here are compositions of matter that comprise DNA analogues that have been rectified by replacing the four standard components with alternative components that mitigate various problems frequently encountered during synthesis of these compounds, during the functioning of these compounds, and complex mixtures for information data storage.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: July 25, 2023
    Inventor: Steven Benner
  • Patent number: 11692192
    Abstract: Described herein are anti-microbial and UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing proteins such as, for example, zinc-related protein/oxidase, silicatein, silaffin, and alcohol dehydrogenase. In some instances, the biological devices also include a gene for lipase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to kill microbial species or prevent microbial growth and to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein. Also disclosed are biological devices producing polyactive carbohydrates and carbo sugars, as well as compositions and articles incorporating both extracts from these devices and the anti-microbial and UV-protective extracts.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: July 4, 2023
    Assignee: BIOCAPITAL HOLDINGS, LLC
    Inventors: Raul Cuero Rengifo, Juliana Londoño Murillo
  • Patent number: 11680281
    Abstract: The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: June 20, 2023
    Assignee: Conagen Inc.
    Inventors: Guohong Mao, Jacob Edward Vick, Michael Batten, David Byun, Yang Luo, Yilin Wu, Beihua Zhang, Xiaodan Yu
  • Patent number: 11680252
    Abstract: A PET hydrolase having improved thermal stability is disclosed. The PET hydrolase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 248 or a corresponding position with proline.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: June 20, 2023
    Assignee: HUBEI UNIVERSITY
    Inventors: Chun-Chi Chen, Jian-Wen Huang, Xiuqin Li, Wei Zeng, Lilan Zhang, Yunyun Yang, Longhai Dai, Jian Min, Yu Yang, Rey-Ting Guo
  • Patent number: 11674162
    Abstract: Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.
    Type: Grant
    Filed: April 8, 2015
    Date of Patent: June 13, 2023
    Assignee: Ginkgo Bioworks, Inc.
    Inventors: Emily H. Greenhagen, Maureen Hamilton, William G. LaTouf, Andrew L. Consiglio, Kyle M. MacEwen, Arthur J. Shaw, IV
  • Patent number: 11674161
    Abstract: The present invention relates to novel monooxygenases which are useful in the hydroxylation of aromatic hydrocarbons. They are particularly useful for the production of 1-naththol and 7-hydroxycoumarin from naphthol and 7-Ethoxycoumarin, respectively.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: June 13, 2023
    Assignee: Covestro Intellectual Property GmbH & Co. KG
    Inventors: Ruijing Guo, Jen-Chieh Lin, Sha Tao, Ying Qian, Chenggang Qiu, Kequan Chen, Kang Li
  • Patent number: 11667682
    Abstract: The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: June 6, 2023
    Assignee: Washington University
    Inventors: Fuzhong Zhang, Christopher H. Bowen, Bin Dai
  • Patent number: 11639497
    Abstract: The present disclosure relates a polypeptide having UGT activity, which polypeptide comprises an amino acid sequence which, when aligned with a polypeptide having UGT activity comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 35, 189, 280, 284, 285, 334 or 373, said positions being defined with reference to SEQ ID NO: 2 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having UGT activity. A polypeptide according to the disclosure may be used in a recombinant cell for the production of steviol or a steviol glycoside.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: May 2, 2023
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Johannes Gustaaf Ernst Van Leeuwen
  • Patent number: 11639505
    Abstract: Described herein are UV-resistant or UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing UV-resistant proteins such as, for example, hexokinase, heat shock proteins, alcohol dehydrogenase, transferrin, flavonol synthase, zinc oxidase, and iron oxidase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 2, 2023
    Assignee: Bio Capital Holdings, LLC
    Inventor: Raul Cuero Rengifo