Patents Examined by Mohammad Y. Meah
  • Patent number: 11549103
    Abstract: Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO2, and/or H2. Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: January 10, 2023
    Assignee: LanzaTech NZ, Inc.
    Inventors: Shilpa Nagaraju, Bakir Al-Sinawi, Sashini De Tissera, Michael Koepke
  • Patent number: 11535833
    Abstract: The present disclosure provides engineered carboxyesterase enzymes that have the ability to catalyze amide bond formation. Also provided are polynucleotides encoding the carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes to make commercially valuable amides. Also provided are amides that are made using the engineered carboxyesterase enzymes.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: December 27, 2022
    Assignee: GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED
    Inventors: Kristin K. Brown, Brent M. Dorr, Douglas E. Fuerst, Katherine Joyce Honicker, Lydia Sanchez Jordan, James Patrick Morrison, Nikolay V. Plotnikov, Markus Schober, Rama Voladri
  • Patent number: 11535878
    Abstract: The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetylglucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate.
    Type: Grant
    Filed: December 26, 2017
    Date of Patent: December 27, 2022
    Assignee: INBIOSE N.V.
    Inventors: Joeri Beauprez, Pieter Coussement, Dries Van Herpe, Gert Peters, Annelies Vercauteren
  • Patent number: 11530426
    Abstract: The disclosure provides a method for biohydrogen production. The method includes: mixing a hydrogen production medium and a buffer solution Na2HPO4/NaH2PO4 having a pH value of 5-9, to yield a first mixture; adding corn stalk powder and cellulase to the first mixture and mixing, to yield a second mixture; adding a suspension of photosynthesis bacteria HAU-M1 at the late exponential phase to the second mixture, to yield a third mixture; and sealing the third mixture and allowing for photo-fermentation biohydrogen production under anaerobic fermentation conditions.
    Type: Grant
    Filed: April 16, 2021
    Date of Patent: December 20, 2022
    Assignee: HENAN AGRICULTURAL UNIVERSITY
    Inventors: Chaoyang Lu, Quanguo Zhang, Zhiping Zhang, Danping Jiang, Yanyan Jing, Yi Wang, Kaixin Wang, Siyi Guo, Jian Wang
  • Patent number: 11518768
    Abstract: The present invention is drawn to artificial metalloenzymes for use in cyclopropanation reactions, amination and C—H insertion.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: December 6, 2022
    Assignee: The Regents of the University of California
    Inventors: John F. Hartwig, Hanna M. Key, Pawel Dydio, Douglas S. Clark
  • Patent number: 11512318
    Abstract: Disclosed is a method for producing 3-fucosyllactose using a wild Corynebacterium glutamicum strain. In addition, using the Corynebacterium glutamicum strain, which is a GRAS strain, 3-fucosyllactose can be produced at a high concentration, high yield and high productivity.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: November 29, 2022
    Assignees: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, ADVANCED PROTEIN TECHNOLOGIES CORP.
    Inventors: Jin-Ho Seo, Sang-Min Jung, Do-Haeng Lee, Hyeong-Do Jeon
  • Patent number: 11512303
    Abstract: The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of ?-hydroxy-?-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed in this invention were developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R)-2-amino-3-hydroxy-3-(4-nitrophenyl) propanoic acid. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing ?-hydroxy-?-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of ?-hydroxy-?-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
    Type: Grant
    Filed: May 9, 2018
    Date of Patent: November 29, 2022
    Assignee: ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.
    Inventors: Haibin Chen, Yong Koy Bong, Qing Xu, Ameng Zhou, Tianran Shen, Jiadong Yang, Zhuhong Yang
  • Patent number: 11492627
    Abstract: The present disclosure provides an innovative method for improving the enzyme activity of an NMN biosynthetic enzyme Nampt, and relates to the technical field of genetic engineering. A mutant protein of the present disclosure is obtained by firstly analyzing a target protein Nampt using two softwares FoldX and DeepDDG, and then predicting multiple key sites influencing the enzyme functions and finally performing the semi-rational design of the enzyme. In the examples of the present disclosure, 10 mutant strains are constructed using the designed primers according to the principle of point mutation, and 8 of the mutants have higher activity than a wild-type strain, in which the NMN yield of the mutant Nampt-V365L is increased by 62%, and the NMN yields of the mutants Nampt-S248A, Nampt-N164L, Nampt-S382M, Nampt-A245T and Nampt-A208G are increased by 34%, 27%, 27%, 22% and 17% respectively.
    Type: Grant
    Filed: September 29, 2021
    Date of Patent: November 8, 2022
    Assignee: HOBOOMLIFE BIO-TECHNOLOGY (SHENZHEN) CO., LTD.
    Inventors: Liqing Zhao, Jiansheng Chen, Zhigang Duan, Haichao Zhang, Beijia Huang
  • Patent number: 11485991
    Abstract: The present invention generally relates to a method of peptides' or polypeptides' modification by glycosylation. In particular, the invention relates to one pot synthesis of disaccharide glycan on to the acceptor substrate and thereby generating O- and/or S-glycosylated neo-glycopeptides including antimicrobial peptides by using multifunctional recombinant nucleotide dependent glycosyltransferase.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: November 1, 2022
    Assignee: Council of Scientific & Industrial Research
    Inventors: Alka Rao, Rupa Nagar
  • Patent number: 11453897
    Abstract: The invention relates to a method for producing aminobenzoic acid or an aminobenzoic acid derivative using a fermentation process, in which (I) the aminobenzoic acid formed in the fermentation broth obtained by the fermentation is bound in part, or as much as possible based on the solubility equilibrium, as insoluble calcium-aminobenzoate, said insoluble calcium-aminobenzoate is then (II) either isolated as such or in a mixture with the microorganism used in the fermentation and transitioned into a water soluble form, while separating an insoluble calcium salt which is different from the calcium-aminobenzoate, and then (III) by introducing carbon dioxide under pressure into the aqueous solution from the precipitated calcium salt has been released, aminobenzoic acid is precipitated.
    Type: Grant
    Filed: June 5, 2019
    Date of Patent: September 27, 2022
    Assignee: Covestro Intellectual Property GmbH & Co. KG
    Inventors: Gernot Jaeger, Guenter Olf, Franz Beggel, Wolf Kloeckner, Simon Klaff
  • Patent number: 11453899
    Abstract: A method produces a protein using a filamentous fungus, in which decrease in dissolved oxygen saturation during culture of the filamentous fungus can be suppressed even when the culture is scaled up. The method of producing a protein includes culturing a fungus belonging to the genus Trichoderma whose BXL1 gene was disrupted, using a biomass containing cellulose and xylan as an inducer. The use of the BXL1 gene-disrupted fungus belonging to the genus Trichoderma enables suppression of the decrease in dissolved oxygen saturation even when xylose and cellulose are used as inducers.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: September 27, 2022
    Assignee: Toray Industries, Inc.
    Inventors: Koji Kobayashi, Shingo Hiramatsu, Katsushige Yamada
  • Patent number: 11447782
    Abstract: The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: September 20, 2022
    Assignee: DANISCO US INC.
    Inventors: Cristina Bongiorni, Marc Kolkman, Chris Leeflang, Virgil Arthur Rhodius, Anita Van Kimmenade
  • Patent number: 11441133
    Abstract: The invention discloses a strain of Acinetobacter and use thereof in the production of chiral 3-cyclohexene-1-carboxylic acid. Its taxonomic name is Acinetobacter sp., which is deposited on Jan. 21, 2019 at the China General Microbiological Culture Collection Center, under accession number CGMCC No. 17220. Using the Acinetobacter strain of the invention to produce chiral methyl 3-cyclohexene-1-carboxylate, the resulting methyl (S)-3-cyclohexene-1-carboxylate has an optical purity of 99% or more, and the catalyst has good stability, mild reaction condition and can withstand high concentrations of substrate and product. Using the resolution process of the invention, (S)-3-cyclohexene-1-carboxylic acid with high optical purity and high concentration can be simply and efficiently obtained, and the process is energy-saving and environmentally friendly, and the high-concentration of product is beneficial to downstream product recovery process.
    Type: Grant
    Filed: November 22, 2019
    Date of Patent: September 13, 2022
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Ye Ni, Zhe Dou, Guochao Xu
  • Patent number: 11434506
    Abstract: The present invention relates to the production of compounds comprised in pheromones, in particular moth pheromones, such as desaturated fatty alcohols and desaturated fatty alcohol acetates and derivatives thereof, from a yeast cell.
    Type: Grant
    Filed: December 15, 2017
    Date of Patent: September 6, 2022
    Assignee: Danmarks Tekniske Universitet
    Inventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
  • Patent number: 11421210
    Abstract: Chimeric and other variant ?-glucuronidase enzymes with enhanced properties as compared to unmodified enzyme are provided. The enzymes of the invention advantageously exhibit enhanced enzymatic activity, enhanced substrate range, enhanced pH range, enhanced temperature range and/or enhanced enzyme stability. Methods of using the variant enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.
    Type: Grant
    Filed: October 8, 2019
    Date of Patent: August 23, 2022
    Assignee: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC.
    Inventors: Caleb Reece Schlachter, John Tomashek, Lim Andrew Lee
  • Patent number: 11407982
    Abstract: Disclosed are transaminase mutants and use thereof. The amino acid sequence of the transaminase mutant is obtained by the mutation of the amino acid sequence as shown in SEQ ID NO: 1, and the mutation at least comprises one of the following mutation sites: the 19-th site, the 41-th site, the 43-th site, the 72-th site, the 76-th site, the 92-th site, the 107-th site, the 125-th site, the 132-th site, the 226-th site, the 292-th site, the 295-th site, the 308-th site, and the 332-th site; and the 19-th site is mutated into a serine, the 41-th site is mutated into a serine, the 43-th site is mutated into an asparagine, a glycine in the 72-th site is mutated into a leucine, etc.; or the amino acid sequence of the transaminase mutant has the mutation sites in the mutanted amino acid sequence, and has more than 80% homology to the mutanted amino acid sequence.
    Type: Grant
    Filed: November 6, 2017
    Date of Patent: August 9, 2022
    Assignee: ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xuecheng Jiao, Na Zhang, Rui Li, Kejian Zhang
  • Patent number: 11396666
    Abstract: A method for producing a compound represented by formula (3) including bringing a carbon-carbon double bond reductase, a microorganism or cell having an ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture solution containing the enzyme which is obtained by culturing the microorganism or cell, and a carbonyl reductase, a microorganism or cell having an ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture solution containing the enzyme which is obtained by culturing the microorganism or cell into contact with a compound represented by formula (1) to obtain a compound represented by formula (3):
    Type: Grant
    Filed: April 17, 2020
    Date of Patent: July 26, 2022
    Assignee: API CORPORATION
    Inventors: Haruka Sasano, Takanobu Iura, Kenji Oki
  • Patent number: 11396646
    Abstract: Polynucleotides encoding corresponding polypeptides capable of glycosylating steviol at its C-19 position to produce a steviol glycoside, an expression vector including such a polynucleotide, a method for producing a steviol glycoside by culturing a recombinant host cell containing such an expression vector under conditions in which the cell expresses the UDP-glycosyltransferase from the polynucleotide, and a method for producing a steviol glycoside by contacting a composition including steviol with a recombinant UDP-glycosyltransferase. The steviol glycoside can be steviol-19-O-glycoside. The recombinant host cell containing such an expression vector can be a bacterial cell, a plant cell, or a fungal cell, an animal cell, or a multicellular organism such as a plant.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: July 26, 2022
    Inventors: Xu Li, Han-Yi Chen, Nickolas Anderson, Amanda Waters
  • Patent number: 11390889
    Abstract: A method for manufacturing 1,3-propanediol includes culturing, in the presence of a saccharide and formaldehyde to produce 1,3-propanediol, a microorganism having the following genes: (a) a first gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and aldehydes; (b) a second gene encoding an enzyme that catalyzes a decarboxylation reaction of ?-keto acids; and (c) a third gene encoding an enzyme that catalyzes a reduction reaction of aldehydes, is provided.
    Type: Grant
    Filed: October 30, 2018
    Date of Patent: July 19, 2022
    Assignees: Green Earth Institute Co., Ltd., Natural Beauty, Limited
    Inventors: Keisuke Yamamoto, Atsunari Tsuchisaka, Shuhei Nakane, Toru Nakayashiki
  • Patent number: 11390894
    Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using polypeptides or recombinant cells comprising said polypeptides. More particularly, the present invention pertains to polypeptides having aryl sulfotransferase activity, recombinant host cells expressing same and processes for the production of aryl sulfates employing these polypeptides or recombinant host cells.
    Type: Grant
    Filed: July 30, 2020
    Date of Patent: July 19, 2022
    Assignee: CysBio ApS
    Inventors: Christian Bille Jendresen, Alex Toftgaard Nielsen