Patents Examined by Mohammad Y. Meah
  • Patent number: 10443078
    Abstract: The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: October 15, 2019
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Jung Hoon Sohn, Hyun Joo Park, Sun Hee Lee, Jung Hoon Bae, Bong Hyun Sung
  • Patent number: 10428334
    Abstract: A heterologous gene cassette useful for creating Streptomyces species with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: October 1, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian Grant Fox, Robert Joseph Stankey, Cameron Robert Currie, Emily Beebe
  • Patent number: 10428360
    Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: October 1, 2019
    Assignee: Codexis, Inc.
    Inventors: Steven J. Collier, Ee Ling Teo, Joly Sukumaran, Robert J. Wilson, Junye Xu
  • Patent number: 10421768
    Abstract: The present invention is related to a biotransformation process, effected by means of an isolated polypeptide possessing benzopyrone phosphate synthetase activity, and also a microorganism comprising a nucleic acid sequence that encodes the polypeptide, for the preparation of phosphate-conjugated derivatives of benzopyrone compounds. The hydrophilic property of the benzopyrone compounds is enhanced after catalyzed by the benzopyrone phosphate synthetase of the present invention.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: September 24, 2019
    Assignee: NATIONAL TAIWAN UNIVERSITY
    Inventors: Nan-Wei Su, Chen Hsu, Shang-Ta Wang
  • Patent number: 10407702
    Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: September 10, 2019
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
  • Patent number: 10385375
    Abstract: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
    Type: Grant
    Filed: January 23, 2017
    Date of Patent: August 20, 2019
    Assignee: Novozymes A/S
    Inventors: Christine Lang, Markus Veen
  • Patent number: 10377722
    Abstract: An object of the present invention is to provide a novel method capable of producing rosuvastatin calcium and intermediates therefor efficiently, inexpensively and with high purity. The present invention provides a method of efficiently producing rosuvastatin calcium and intermediates therefor having a high purity at an industrial scale, without using an extremely low temperature reaction or a special asymmetric catalyst.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: August 13, 2019
    Assignee: API CORPORATION
    Inventors: Naoyuki Watanabe, Tomoko Maeda, Yasumasa Dekishima, Hiroshi Kawabata, Masaki Nagahama, Kosuke Ito
  • Patent number: 10358667
    Abstract: Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: July 23, 2019
    Assignees: University of Maryland, Baltimore, University of Washington through its Center for Commercialization
    Inventors: Robert K. Ernst, Mark Pelletier, Adeline Hajjar
  • Patent number: 10337144
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: July 2, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen
  • Patent number: 10316106
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: June 11, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen
  • Patent number: 10308925
    Abstract: Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No.1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 ?M MTL·h?1·mg protein?1.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: June 4, 2019
    Assignee: HUBEI UNIVERSITY OF TECHNOLOGY
    Inventors: Yajie Tang, Kaizhi Jia, Yanghua Xu, Quan Zhang, Hongmei Li
  • Patent number: 10287289
    Abstract: The application is directed to efficient and economical processes as described in more detail below for the preparation of the beta 3 agonists of the formula of I-7 and intermediate compounds that can be used for making these agonists. The present disclosure relates to a process for making beta-3 agonists and intermediates using ketoreductase (KRED) biocatalyst enzymes and methods of using the biocatalysts.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: May 14, 2019
    Assignee: MERCK SHARP & DOHME CORP.
    Inventors: Feng Xu, Zhuqing Liu, Richard Desmond, Jeonghan Park, Alexei Kalinin, Birgit Kosjek, Hallena Strotman, Hongmei Li, Johannah Moncecchi
  • Patent number: 10280441
    Abstract: The present invention provides for a method of producing a cinnamoyl anthranilate, or analog thereof, in a genetically modified host cell.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: May 7, 2019
    Assignee: The Regents of the University of California
    Inventors: Dominique Loque, Aymerick Guillaume Eudes
  • Patent number: 10253303
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: April 9, 2019
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 10253344
    Abstract: Recombinant polypeptides having UDP-glycosyltransferase activities, including a 1,2-19-O-glucose glycosylation activity and a 1,2-13-O-glucose glycosylation activity for synthesizing of steviol glucosides, are provided. A method of producing a steviol glycoside composition using such recombinant polypeptide is also provided. Also disclosed are steviol glycosides referred to as rebaudioside Z1 and rebaudioside Z2.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: April 9, 2019
    Assignee: Conagen Inc.
    Inventors: Guohong Mao, Xiaodan Yu
  • Patent number: 10246687
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: April 2, 2019
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 10233467
    Abstract: Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.
    Type: Grant
    Filed: August 14, 2013
    Date of Patent: March 19, 2019
    Assignee: Easel Biotechnologies, LLC
    Inventors: Yi-Xin Huo, Kwang Myung Cho
  • Patent number: 10227622
    Abstract: The present invention provides for a genetically modified host cell comprising a 2-pyrrolidone synthase, or an enzymatically active fragment thereof, heterologous to the host cell.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 12, 2019
    Assignee: The Regents of the University of California
    Inventors: Jingwei Zhang, Jay D. Keasling
  • Patent number: 10221400
    Abstract: Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d?1, and a cell density of less than about 2×107 cell/mL.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: March 5, 2019
    Assignees: Baxalta GmbH, Baxalta Incorporated
    Inventors: Leopold Grillberger, Manfred Reiter, Daniel Fleischanderl
  • Patent number: 10202621
    Abstract: Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels, carboxylic acids and equivalents thereof (e.g., esters and salts). For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol, butanol or organic acids (e.g., acetic or lactic acid), salts of organic acids or mixtures thereof. If desired, organic acids can be converted into alcohols, such as by first converting the acid, salt or mixtures of the acid and its salt to an ester, and then hydrogenating the formed ester. Acetogens or homoacetogens which are capable of utilizing a syngas from a thermochemical conversion of coal or biomass can be utilized to produce the desired product.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: February 12, 2019
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman