Patents Examined by Mohammad Y. Meah
  • Polypeptides for carbon-carbon bond formation and uses thereof
    Patent number: 10570379
    Abstract: This document describes polypeptides with dual CoA transferase and ?-ketothiolase activities and variants thereof, use of such polypeptides in biosynthetic methods, and non-naturally occurring hosts comprising such polypeptides.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: February 25, 2020
    Assignee: INVISTA North America S.a.r.l.
    Inventors: Nadia Kadi, Adriana Leonora Botes
  • Multi-enzyme conjugate, method for preparing the same and method for preparing organic compound using the same
    Patent number: 10563230
    Abstract: The present disclosure relates to a multi-enzyme conjugate, a method for preparing the same and a method for preparing an organic compound using the same. More particularly, a multi-enzyme conjugate exhibiting improved catalytic efficiency over respective free enzymes using site-specific incorporation of a clickable non-natural amino acid into the enzymes and two compatible click reactions, a method for preparing the same and a method for preparing an organic compound using the same may be provided.
    Type: Grant
    Filed: May 26, 2017
    Date of Patent: February 18, 2020
    Assignee: GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Inchan Kwon, Jinhwan Cho, Sung In Lim
  • Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
    Patent number: 10544402
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: January 28, 2020
    Assignee: Codexis Inc.
    Inventors: Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier
  • Ketoreductase polypeptides
    Patent number: 10544401
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: January 28, 2020
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Biocatalysts for ezetimibe synthesis
    Patent number: 10544400
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: January 28, 2020
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Methods and products for production of wax esters
    Patent number: 10533198
    Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: January 14, 2020
    Assignee: BIOPETROLIA AB
    Inventors: Jens Nielsen, Shuobo Shi
  • Streptomyces and method for producing milbemycin A4 using same
    Patent number: 10526668
    Abstract: Provided are a Streptomyces (Streptomyces hygroscopicus) HS7522 and a method for preparing milbemycin A4 by culturing the Streptomyces. The Streptomyces (Streptomyces hygroscopicus) HS7522 of the present invention is deposited in “China General Microbiological Culture Collection Center” with an accession number of CGMCC No. 9671 on Sep. 16, 2014.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: January 7, 2020
    Assignee: Zhejiang Hisun Pharmaceutical Co., Ltd.
    Inventors: Yun Teng, Meidong Xu, Meiyi Mo, Zhihui He, Zhengjie Chen, Lianiqing Jiang, Hua Bai
  • Universal N-glycan binding reagent
    Patent number: 10507233
    Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: December 17, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
  • Modified glucosyltransferases for producing branched alpha-glucan polymers
    Patent number: 10508268
    Abstract: Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
    Type: Grant
    Filed: April 18, 2018
    Date of Patent: December 17, 2019
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Mark S. Payne, Yefim Brun, Richard R. Bott
  • Enone reductases
    Patent number: 10494615
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: December 3, 2019
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • NG7 microorganism and uses thereof
    Patent number: 10443078
    Abstract: The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: October 15, 2019
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Jung Hoon Sohn, Hyun Joo Park, Sun Hee Lee, Jung Hoon Bae, Bong Hyun Sung
  • Lov-D acyltransferase mediated acylation
    Patent number: 10428360
    Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: October 1, 2019
    Assignee: Codexis, Inc.
    Inventors: Steven J. Collier, Ee Ling Teo, Joly Sukumaran, Robert J. Wilson, Junye Xu
  • Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates
    Patent number: 10428334
    Abstract: A heterologous gene cassette useful for creating Streptomyces species with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: October 1, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian Grant Fox, Robert Joseph Stankey, Cameron Robert Currie, Emily Beebe
  • Method of biotransformation of benzopyrone compounds into the corresponding phosphate-conjugated derivatives
    Patent number: 10421768
    Abstract: The present invention is related to a biotransformation process, effected by means of an isolated polypeptide possessing benzopyrone phosphate synthetase activity, and also a microorganism comprising a nucleic acid sequence that encodes the polypeptide, for the preparation of phosphate-conjugated derivatives of benzopyrone compounds. The hydrophilic property of the benzopyrone compounds is enhanced after catalyzed by the benzopyrone phosphate synthetase of the present invention.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: September 24, 2019
    Assignee: NATIONAL TAIWAN UNIVERSITY
    Inventors: Nan-Wei Su, Chen Hsu, Shang-Ta Wang
  • Biocatalytic transamination process
    Patent number: 10407702
    Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: September 10, 2019
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
  • Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms
    Patent number: 10385375
    Abstract: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
    Type: Grant
    Filed: January 23, 2017
    Date of Patent: August 20, 2019
    Assignee: Novozymes A/S
    Inventors: Christine Lang, Markus Veen
  • Rosuvastatin calcium and process for producing intermediate thereof
    Patent number: 10377722
    Abstract: An object of the present invention is to provide a novel method capable of producing rosuvastatin calcium and intermediates therefor efficiently, inexpensively and with high purity. The present invention provides a method of efficiently producing rosuvastatin calcium and intermediates therefor having a high purity at an industrial scale, without using an extremely low temperature reaction or a special asymmetric catalyst.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: August 13, 2019
    Assignee: API CORPORATION
    Inventors: Naoyuki Watanabe, Tomoko Maeda, Yasumasa Dekishima, Hiroshi Kawabata, Masaki Nagahama, Kosuke Ito
  • Immunotherapeutic potential of modified lipooligosaccharides/lipid A
    Patent number: 10358667
    Abstract: Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: July 23, 2019
    Assignees: University of Maryland, Baltimore, University of Washington through its Center for Commercialization
    Inventors: Robert K. Ernst, Mark Pelletier, Adeline Hajjar
  • Method for processing cellulose-containing biomass
    Patent number: 10337144
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: July 2, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen
  • Method for processing cellulose-containing biomass
    Patent number: 10316106
    Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: June 11, 2019
    Assignee: BASF SE
    Inventors: Frank Rittig, Stefan Koch, Alois Kindler, Michael Koch, Ferdinand Leifeld, Vaidotas Navickas, Markus Gruen