Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.
Abstract: A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.
Type:
Grant
Filed:
January 22, 2018
Date of Patent:
November 2, 2021
Assignee:
JILIN ASYMCHEM LABORATORIES CO., LTD.
Inventors:
Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Na Zhang, Yulei Ma, Yibing Cheng, Huiyan Mu
Abstract: A method for producing an objective substance such as sphingoid bases and sphingolipids using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance in a culture medium containing an additive that is able to associate with, bind to, solubilize, and/or capture the objective substance, and collecting the objective substance from cells of the yeast and/or the culture medium.
Type:
Grant
Filed:
February 16, 2018
Date of Patent:
October 26, 2021
Assignees:
Ajinomoto Co., Inc., Evolva SA
Inventors:
Markus Schwab, Corina Daniela Wirdnam, Thomas Oestergaard Tange, Maud Babau, Anaelle Hatsch, Fanny Delegrange, David Fischer, Sabina de Andrade Pereira Tavares
Abstract: The present invention provides a method for the production of oligosaccharides by the fermentation of dextran-sucrase-producing microorganisms with sucrose and maltose. The disclosed process allows for the control of the final composition of the isomaltooligosaccharides by adjustments to pH and the initial ratio of sucrose to maltose.
Type:
Grant
Filed:
August 22, 2015
Date of Patent:
October 26, 2021
Assignee:
ISOThrive Inc.
Inventors:
Lee Madsen, II, Jack Oswald, Donal F Day, Young Hwan Moon
Abstract: Disclosed herein are methods that may be used for the synthesis of benzylisoquinoline alkaloids (BIAs) such as alkaloid morphinan. The methods disclosed can be used to produce thebaine, oripavine, codeine, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone, naltrexone, naloxone, hydroxycodeinone, neopinone, and/or buprenorphine. Compositions and organisms useful for the synthesis of BIAs, including thebaine synthesis polypeptides, purine permeases, and polynucleotides encoding the same, are provided.
Type:
Grant
Filed:
June 27, 2017
Date of Patent:
October 12, 2021
Assignee:
Antheia, Inc.
Inventors:
Peter James Facchini, Xue Chen, Jeffrey C. Colbeck, Joseph Tucker
Abstract: The present invention discloses a ?-galactosidase mutant, and a preparation method and application thereof, belonging to the fields of gene engineering and enzyme engineering. Amino acids of specific sites in the ?-galactosidase are mutated, the ?-galactosidase is transferred into a recombinant bacterium, and enzymatic transformation is performed under optimized conditions, so that the yield of galactooligosaccharide produced by the mutant reaches 59.8%, which is increased by about 70% as compared with that of wild enzyme, thereby implementing the increase of the galactooligosaccharide yield. The present invention has very high industrialized application value.
Abstract: This Invention discloses a method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt by microbial fermentation. The method is intended to manufacture N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt in higher efficiency and higher yield, by expression of vitreoscilla hemoglobin in microorganism.
Abstract: The invention provides an improved method of isolating the 3-(E)-isomer of an unsaturated carboxylic acid from a mixture of corresponding (E/Z)isomers. More particularly, the present invention relates to an improved method for the biocatalytic preparation of (3E,7E)-homofarnesylic acid; as well as a novel biocatalytic method for the improved preparation of homofarnesol, in particular of (3E,7E)-homofarnesol and homofarnesol preparations having an increased content of (3E,7E)-homofarnesol. The present invention also relates to methods of preparing(?)-ambroxby applying (3E,7E)-homofarnesylic acid or (3E,7E)-homofarnesol as obtained according to the invention as starting material.
Type:
Grant
Filed:
February 23, 2018
Date of Patent:
October 5, 2021
Assignee:
BASF SE
Inventors:
Wolfgang Siegel, Melanie Weingarten, Michael Breuer, Mathias Schelwies
Abstract: Disclosed in the present invention is a recombinant Escherichia coli and application thereof. The recombinant Escherichia coli was deposited on Aug. 28, 2018 in the China General Microbiological Culture Collection Center with the preservation number CGMCC No. 16349. The recombinant Escherichia coli is fermented and cultured, and the concentration of Microcin J25 in the supernatant can reach 4000 mg/L or more.
Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
Type:
Grant
Filed:
October 3, 2016
Date of Patent:
October 5, 2021
Assignee:
DSM IP Assets B.V.
Inventors:
Ioannis Papapetridis, Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Paul Klaassen
Abstract: Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.
Type:
Grant
Filed:
June 4, 2019
Date of Patent:
September 21, 2021
Assignees:
UNIVERSITY OF MARYLAND, BALTIMORE, UNIVERSITY OF WASHINGTON
Inventors:
Robert K. Ernst, Mark Pelletier, Adeline Hajjar
Abstract: A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having ?, ?-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes.
Type:
Grant
Filed:
July 4, 2016
Date of Patent:
September 21, 2021
Assignee:
The Governing Council of The University of Toronto
Inventors:
Alexander Yakunin, Anna Khusnutdinova, Jeong Chan Joo, Radhakrishnan Mahadevan
Abstract: The present invention relates to methods and compositions for modulating glycosylation profile, such as the galactosylation profile, of recombinant proteins expressed by mammalian host cells during the cell culture process by supplementing cell culture media with a peracetyl galactose.
Type:
Grant
Filed:
May 10, 2017
Date of Patent:
September 14, 2021
Assignee:
ARES TRADING S.A.
Inventors:
Martin Jordan, Gabrielle Leclercq, Jonathan Souquet, Herve Broly, David Bruhlmann
Abstract: This invention claims processes that append a single nucleotide having a 3?-ONH2 moiety to the 3?-ends of an oligonucleotide primer using 3?-deoxynucleoside triphosphates that have, instead of a 3?-OH moiety, a 3?-ONH2 moiety, where the nucleotides contain both standard and non-standard nucleobases, and where as a key claim limitation, substantially no hydroxylamine is present in the solutions used in the claimed processes.
Abstract: The present invention relates to the preparation of amines from aldehydes and ketones by reductive amination with enzymes having a reductive aminase activity on aldehydes and ketones devoid of any carboxyl group gamma of the carbonyl group. The invention also relates to the enzymes per se and their uses in biocatalysis. The enzymes are derived from Mycobacterium smegmatis and vaccae, Cystobacter fuscus, Microbacterium sp. and Aminomonas paucivorans.
Type:
Grant
Filed:
July 5, 2018
Date of Patent:
September 14, 2021
Assignees:
COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, UNIVERSITE D'EVRY VAL D'ESSONNE (UEVE)
Inventors:
Carine Vergne-Vaxelaire, Véronique De Berardinis, Anne Zaparucha
Abstract: The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume gluconse and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
September 7, 2021
Assignee:
CARGILL, INCORPORATED
Inventors:
Arlene M. Fosmer, Christopher K. Miller, Gregory Michael Poynter, Brian Jeffrey Rush, Jon Veldhouse
Abstract: The present invention discloses a fermentation process with a Pichia yeast expressing a recombinant protein, in which Pichia pastoris is used as a fungal strain. The process comprises performing primary seed culturing to reach a fungal concentration of 20±2 g/L; then performing secondary seed culturing to reach a fungal concentration of 120±10 g/L; next proceeding to a glycerol culturing stage, wherein the amount of glycerol added in the glycerol culturing stage is 60-70 g/L; and then proceeding to a methanol-induced stage for 120±8 h after the dissolved oxygen quickly reaches a relatively stable state, to complete the fermentation process. In the present invention, a glycerol fed-batch addition stage in existing processes is omitted, and the process proceeds to a next stage as soon as glycerol is completely consumed, with no need to prepare sterilized glycerol for fluidic addition. As such, the glycerin sterilizer is omitted, the consumption of energy and resource and the waste of glycerin are reduced.
Type:
Grant
Filed:
October 18, 2016
Date of Patent:
August 31, 2021
Assignee:
JIANGSU JLAND BIOTECH CO., LTD.
Inventors:
Shulin Yang, Jianfeng Zhao, Jianmin Huang, Lihu Gao, Erfeng Du, Hai Tao, Liping Feng, Le Ji, Aimei Zhou
Abstract: An enzyme having the following characteristics (a) and (b): (a) the enzyme has an activity of reversible dehydrogenation of D-amino acids; (b) the enzyme is a hexamer of polypeptides having an amino acid sequence having 80% or greater identity to the amino acid sequence of SEQ ID NO: 2.
Type:
Grant
Filed:
August 9, 2018
Date of Patent:
August 24, 2021
Assignees:
KUREHA CORPORATION, NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
Type:
Grant
Filed:
February 13, 2020
Date of Patent:
August 24, 2021
Assignee:
Codexis, Inc.
Inventors:
Martina Quintanar-Audelo, Ellen Eberhard, Jovana Nazor, Derek Smith, Cuixia Wang
Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
Type:
Grant
Filed:
December 9, 2019
Date of Patent:
August 24, 2021
Assignee:
Codexis, Inc.
Inventors:
Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier