Patents Examined by Mohammad Younus Meah
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Patent number: 8999705Abstract: This invention provides nucleic acids and proteins involved in oligosaccharide modification in the species Bifidobacteria. The invention provides methods for utilizing the proteins of the invention to generate human milk oligosaccharides or oligosaccharide mimics. The invention also provides compositions containing the human milk oligosaccharides or oligosaccharide mimics and methods for use.Type: GrantFiled: December 19, 2012Date of Patent: April 7, 2015Assignee: The Regents of the University of CaliforniaInventors: David A. Mills, Carlito B. Lebrilla, J. Bruce German, David Sela
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Patent number: 8178321Abstract: This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid/liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm/Abs 280 nm).Type: GrantFiled: August 16, 2007Date of Patent: May 15, 2012Assignee: Instituto Tecnologico y de Estudios Superiores de MonterreyInventors: Marco Antonio Rito Palomares, Jorge Alejandro Benavides Lozano, Tanhia Denys Hernandez Mireles
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Patent number: 7666658Abstract: Nine efficient aldolase antibodies were generated using hapten 2. This hapten combines, in a single molecule, structural components employed for reactive immunization with structural components employed for forming a transition state analog of the aldol reaction. Characterization of two of these antibodies reveals that they are highly proficient (up to 1000-fold better than any other antibody catalyst) and enantioselective catalysts for aldol and retro-aldol reactions and exhibit enantio- and diastereo-selectivities opposite that of antibody 38C2.Type: GrantFiled: October 6, 2000Date of Patent: February 23, 2010Assignee: The Scripps Research InstituteInventors: Carlos F. Barbas, Richard A. Lerner, Guofu Zhong
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Patent number: 7585650Abstract: The invention relates to mutants and alleles of the zwf gene of coryneform bacteria, which encode variants of the Zwf subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.Type: GrantFiled: April 23, 2007Date of Patent: September 8, 2009Assignee: Degussa AGInventors: Brigitte Bathe, Natalie Schischka, Georg Thierbach
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Patent number: 7575909Abstract: The invention relates to a NADH-dependent oxidoreductase obtained from yeasts, for example, of the genus Pichia, more particularly from Pichia capsulata. The oxidoreductace used in an enzymatic process for enantioselective reduction of organic ketocompounds to the corresponding (S)-hydroxy compounds and in an enzymnatic process for an antioselective preparation of (S)-hydroxy compounds in a two phase system using the isolated, recombinantly overexpressed oxidoreductacse from Pichia capsulata.Type: GrantFiled: May 28, 2004Date of Patent: August 18, 2009Assignee: IEP GmbHInventors: Antje Gupta, Anke Tschentscher, Maria Bobkova
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Patent number: 7572612Abstract: A thermostable TAL enzyme was identified from the fungus Phanerochaete chrysosporium, which has high activity at temperatures of 40° C. to about 60° C. The enzyme was produced in engineered cells and used for production of para-hydroxycinnamic acid (pHCA) from tyrosine. When the pHCA production reaction was run at high temperature, the enzyme was more active and pHCA was produced more rapidly, making the reaction more efficient.Type: GrantFiled: July 12, 2006Date of Patent: August 11, 2009Assignee: E.I. du Pont de Nemours and CompanyInventors: Lixuan Lisa Huang, Zhixiong Xue, Michael P. McCluskey
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Patent number: 7553656Abstract: It is described biologically pure bacterial cultures of mutant strains of Pseudomonas fluorescens, which produces large amounts of alginate. The alginate may contain a certain determined content of mannuronate and guluronate residues, possible presence and determined level of acetyl groups in the alginate, and a desired molecular weight of the alginate. Also high yielding mutants with regulation of alginate production, is described. The invention further provides methods for producing new mutant strains of Pseudomonas fluorescens and variants thereof, and use the resulting strains in alginate production.Type: GrantFiled: July 24, 2003Date of Patent: June 30, 2009Assignee: FMC Biopolymer ASInventors: Martin Gimmestad, Havard Sletta, Karuna Ponniah Karunakaran, Karianne Bakkevig, Helga Ertesvag, Trond Ellingsen, Gudmund Skjak-Braek, Svein Valla
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Patent number: 7553652Abstract: The present invention relates to a novel process for the preparation of (S)— or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid and to novel microorganisms capable of utilizing the propionamide of the formula in the form of the racemate or of its optically active isomers as the sole nitrogen source.Type: GrantFiled: August 10, 2005Date of Patent: June 30, 2009Assignee: Lonza AGInventors: Walter Brieden, Andrew Naughton, Karen Robins, Nicholas Shaw, Andreas Tinschert, Thomas Zimmermann
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Patent number: 7531331Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.Type: GrantFiled: August 30, 2001Date of Patent: May 12, 2009Assignee: BASF AktiengesellschaftInventors: Bernhard Hauer, Thomas Friedrich, Christoph Nübling, Rainer Stürmer, Wolfgang Ladner
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Patent number: 7524644Abstract: The present invention relates to a nucleotide sequence of expression cassette OXY-1 of SEQ ID No. 1, a modified staphylokinase SAK-2 gene of SEQ ID No. 2, a peptide sequence of modified staphylokinase SAK-2 gene, of SEQ ID No. 3, three plasmids having International Deposition Nos. BPL-0019, BPL-0020, and BPL-0021, and their corresponding three recombinant E. Coli; also invention relates to a process for over-producing staphylokinase and its analogues by modulating level of oxygen of its growth medium in a host system, and lastly, a method of dissolving blood clot in a subject in need thereof.Type: GrantFiled: March 31, 2004Date of Patent: April 28, 2009Assignee: Council of Scientific and Industrial ResearchInventors: Govindan Rajamohan, Monika Dahiya, Ranjana Pathania, Kanak Lata Dikshit
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Patent number: 7524655Abstract: This invention provides nucleic acid and amino acid sequences of fucosyltransferases from Helicobactor pylori. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids.Type: GrantFiled: December 6, 2005Date of Patent: April 28, 2009Assignees: Neose Technologies, Inc., Governors of the University of AlbertaInventors: Karl F. Johnson, Daniel James Bezila, Diane E. Taylor, Joanne Simala-Grant, David Rasko
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Patent number: 7491521Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.Type: GrantFiled: August 20, 2007Date of Patent: February 17, 2009Assignee: Evonik Degussa GmbHInventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
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Patent number: 7432094Abstract: A DNA fragment encoding a ? subunit is obtained by inverse PCR using primers designed based on the nucleotide sequence of a N-terminal signal sequence region of a GDH ? subunit derived from Burkholderia cepacia KS1 strain.Type: GrantFiled: May 31, 2006Date of Patent: October 7, 2008Inventor: Koji Sode
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Patent number: 7429469Abstract: This invention relates to a method of producing polyesters resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties using yeasts as hosts. By constructing at least one enzyme gene involved in polyester synthesis by adding a DNA coding a peroxisome-targeting signal, introducing an enzyme gene expression cassette containing that gene into yeast, and cultivating the thus-obtained transformant, it becomes possible to cause accumulation of a polyester resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) in yeast cells and recover the polyester from the culture.Type: GrantFiled: October 9, 2002Date of Patent: September 30, 2008Assignee: Kaneka CorporationInventors: Satoru Yokomizo, Takeshi Fukuchi, Fumio Osakada, Keiji Matsumoto, Masamichi Takagi, Akinori Ohta
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Patent number: 7241599Abstract: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).Type: GrantFiled: June 7, 2005Date of Patent: July 10, 2007Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Kenichi Mori, Hiroyuki Nozaki
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Patent number: 7220564Abstract: The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence shown under SEQ ID NO:1 in the sequence listing, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.Type: GrantFiled: September 19, 2003Date of Patent: May 22, 2007Assignee: Kaneka CorporationInventors: Noriyuki Kizaki, Tozo Nishiyama, Yoshihiko Yasohara