Patents Examined by Narayan K Bhat
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Patent number: 11974730Abstract: In some embodiment, the present invention is directed to a device for obtaining samples from a subject. In other embodiments, methods and devices of the present invention allow the evaluation of conditions of the gastrointestinal tract of a subject. Measurements may be utilized, for example, to diagnose a disease of the esophagus, to monitor inflammation of the esophagus, or to access the treatment of a disease of the esophagus. In one embodiment, the invention comprises a method for measuring esophageal inflammation comprising deploying a device into the esophagus of a subject, removing the device after a predetermined period of time, analyzing the device for a diagnostic indicator of esophageal inflammation and evaluating the diagnostic indicator to diagnose esophageal inflammation.Type: GrantFiled: May 11, 2020Date of Patent: May 7, 2024Assignees: The Regents of the University of Colorado, The Board of Trustees of the University of IllinoisInventors: Glenn T. Furuta, Steven J. Ackerman
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Patent number: 11976272Abstract: The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.Type: GrantFiled: October 14, 2020Date of Patent: May 7, 2024Assignee: TRANSLATE BIO, INC.Inventors: Jonathan Abysalh, Daniel Crawford, Frank DeRosa, Shrirang Karve, Anusha Dias, Michael Heartlein
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Patent number: 11969731Abstract: Instruments, methods, and kits are disclosed for performing fast thermocycling.Type: GrantFiled: November 24, 2020Date of Patent: April 30, 2024Assignee: Biofire Defense, LLCInventors: Kirk Max Ririe, David E. Jones, Christopher Paul Pasko, Anson Cole Chamberlain, Derek David, Aaron Wernerehl, Jonathan Allen Bruns
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Patent number: 11946100Abstract: The present application provides a microdroplet container and a method for manufacturing the same, a method for spreading microdroplets, a microdroplet-generating kit, a temperature-controlling device, an oil phase composition for microdroplet generating and a method for treating the same. The temperature controlling device includes a flexible circuit board a heating substrate, and multiple semiconductor electric couples. The semiconductor electric couples are disposed between the flexible circuit board and a first surface of the heating substrate, and are connected to each other.Type: GrantFiled: January 24, 2019Date of Patent: April 2, 2024Assignee: SNIPER (SUZHOU) LIFE TECHNOLOGY CO., LTD.Inventor: Guang-Ji Sheng
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Patent number: 11939638Abstract: Method and systems for identifying and distinguishing subjects using a detection system comprising pooled probes are described. The pooled probes described comprise subject specific features allowing for identification and distinction of subjects in a complex sample and can be utilized in a variety of detection platforms. The methods and systems can further comprise the use of isotachophoresis to concentrate analytes in a sample.Type: GrantFiled: May 8, 2018Date of Patent: March 26, 2024Assignee: REVERE BIOSENSORS, LLCInventors: Robert Charles Kain, Theofilos Kotseroglou, Vladimir Bashkirov, Richard Shen
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Patent number: 11939625Abstract: A method and a sensor for detecting L-cystine are disclosed. The method is implemented by assembling a sodium 3,3?-dithiodipropane sulfonate (SPS) membrane on a surface of Au membrane layer of an Au electrode and using an extended gate of field effect transistor (FET) and in-situ signal amplification of the FET to detect L-cystine sensitively. The polyanion of the SPS membrane adsorbs and binds a positively charged target L-cystine through electrostatic interaction, thus forming an electric double layer structure to generate a membrane potential identifying a monovalent organic ammonium ion. The sensor includes the FET, wherein a gate-extended gold electrode is arranged on the FET, and the SPS membrane is assembled on the surface of the Au membrane layer of the gate-extended gold electrode. The sensor has an excellent Nernst response to L-cystine.Type: GrantFiled: August 24, 2019Date of Patent: March 26, 2024Assignee: CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGYInventors: Zhong Cao, Jia Yang, Chen Liu, Zhongliang Xiao, Dan Li, Ling Zhang, Yuyang Zhang, Jiaxin Li
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Patent number: 11941534Abstract: A system is provided that includes a bit vector-based distance counter circuitry configured to generate one or more bit vectors encoded with information about potential matches and edits between a read and a reference genome, wherein the read comprises an encoding of a fragment of deoxyribonucleic acid (DNA) encoded via bases G, A, T, C. The system further includes a bit vector-based traceback circuitry configured to divide the reference genome into one or more windows and to use the plurality of bit vectors to generate a traceback output for each of the one or more windows, wherein the traceback output comprises a match, a substitution, an insert, a delete, or a combination thereof, between the read and the one or more windows.Type: GrantFiled: December 28, 2019Date of Patent: March 26, 2024Assignee: Intel CorporationInventors: Gurpreet Singh Kalsi, Anant V. Nori, Christopher Justin Hughes, Sreenivas Subramoney, Damla Senol
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Patent number: 11939627Abstract: Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.Type: GrantFiled: March 23, 2021Date of Patent: March 26, 2024Assignee: Bionano Genomics, Inc.Inventors: Ming H. Xiao, Han Cao
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Patent number: 11940382Abstract: A homogeneous assay method that employs a device is provided. In some embodiments, the device contains a pair of plates that can be opened and closed. The sample is placed between two plates. In some embodiments, the thickness of the sample in a closed configuration, the concentration of labels, and amplification factor of the amplification surface are configured to make the label(s) bound on the amplification surface visible without washing away of the unbound labels.Type: GrantFiled: February 8, 2018Date of Patent: March 26, 2024Assignee: Essenlix CorporationInventors: Stephen Y. Chou, Wei Ding, Yufan Zhang, Ji Qi, Ji Li
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Patent number: 11932901Abstract: Disclosed herein include systems, methods, compositions, and kits for performing targeted single cell mRNA sequencing assays. Capture oligonucleotides capable of specifically binding to target nucleic acid species are provided in some embodiments. Depletion oligonucleotides capable of specifically binding to undesirable nucleic acid species are provided in some embodiments. The method can comprise random priming and extension of barcoded transcripts followed by one or more enrichment and/or depletion steps using said capture oligonucleotides and/or depletion oligonucleotides. Immune repertoire profiling methods are also provided in some embodiments.Type: GrantFiled: July 12, 2021Date of Patent: March 19, 2024Assignee: Becton, Dickinson and CompanyInventors: Hye-Won Song, Margaret Nakamoto
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Patent number: 11932906Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.Type: GrantFiled: June 17, 2021Date of Patent: March 19, 2024Assignee: Quantum-Si IncorporatedInventors: Guojun Chen, Jeremy Lackey, Alexander Goryaynov, Gerard Schmid, Ali Kabiri, Jonathan M. Rothberg, Todd Rearick, Jonathan C. Schultz, Farshid Ghasemi, Keith G. Fife
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Patent number: 11932899Abstract: Provided herein are methods and systems for characterizing a nucleic acid molecule from a single cell. A method for characterizing a nucleic acid molecule from a single cell may comprise providing a partition (e.g., droplets or wells) comprising a single cell and a single bead. The single bead may comprise a nucleic acid barcode molecule. A nucleic acid molecule from the single cell and the nucleic acid barcode molecule may be used to generate a barcoded nucleic acid molecule for sequencing, which may be used to determine an epigenetic state or characteristic of the nucleic acid molecule as being associated with the single cell.Type: GrantFiled: June 6, 2019Date of Patent: March 19, 2024Assignee: 10X GENOMICS, INC.Inventors: Kamila Belhocine, Michael Schnall-Levin, Andrew D. Price
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Patent number: 11932904Abstract: Disclosed herein are improved methods and systems for sequencing nucleic acid that exploit the temperature-dependence of the emitted intensity of fluorescent dyes. The temperature of the sequencing reaction is adjusted during each sequencing cycle, and the emission, or lack of emission, of light meeting or exceeding a threshold by the fluorescent dyes at different temperatures, or within different temperature ranges, is used to detect the fluorescent labels of the incorporated dNTPs and thereby sequence the nucleic acid. The disclosed methods enable a determination of the dNTP incorporated at any given site with a reasonable number of chemistry steps without the complex optics necessary for prior-art systems.Type: GrantFiled: December 2, 2021Date of Patent: March 19, 2024Assignee: Western Digital Technologies, Inc.Inventors: Patrick Braganca, Daniel Bedau
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Patent number: 11926867Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.Type: GrantFiled: March 16, 2023Date of Patent: March 12, 2024Assignee: 10x Genomics, Inc.Inventors: Yifeng Yin, Michael Schnall-Levin, Eswar Prasad Ramachandran Iyer
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Patent number: 11920184Abstract: Methods and related products are disclosed that improve the probability of interaction between a target molecule and a nanopore by capturing the target molecule on a surface comprising the nanopore. The captured target molecule, the nanopore, or both, are able to move relative to each other along the surface. When the leader of the target molecule is in proximity with the nanopore, interaction of the target portion of the target molecule with the nanopore occurs, thereby permitting sensing of the target portion. Confining the target molecule and nanopore in this manner leads to significantly enhanced interaction with the nanopore.Type: GrantFiled: October 15, 2020Date of Patent: March 5, 2024Assignee: Roche Sequencing Solutions, Inc.Inventors: Mark Stamatios Kokoris, Robert N. McRuer
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Patent number: 11911765Abstract: A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.Type: GrantFiled: June 16, 2020Date of Patent: February 27, 2024Assignee: ILLUMINA, INC.Inventors: Tony Yen, Eric Stava, Rajagopal Panchapakesan
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Patent number: 11905558Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.Type: GrantFiled: August 14, 2020Date of Patent: February 20, 2024Assignee: Life Technologies CorporationInventors: Mark Reed, Chiu Tai Andrew Wong, David Marran
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Patent number: 11891669Abstract: Method and systems for identifying and distinguishing subjects using a detection system comprising pooled probes are described. The pooled probes described comprise subject specific features allowing for identification and distinction of subjects in a complex sample and can be utilized in a variety of detection platforms. The methods and systems can further comprise the use of isotachophoresis to concentrate analytes in a sample.Type: GrantFiled: May 8, 2018Date of Patent: February 6, 2024Assignee: REVERE BIOSENSORS, LLCInventors: Robert Charles Kain, Theofilos Kotseroglou, Vladimir Bashkirov, Richard Shen
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Patent number: 11892444Abstract: Improved multi-cell nanopore-based sequencing chips and methods can employ formation, characterization, calibration, and/or normalization techniques. For example, various methods may include one or more steps of performing physical checks of cell circuitry, forming and characterizing a lipid layer on the cells, performing a zero point calibration of the cells, forming and characterizing nanopores on the lipid layers of each cell, performing a sequencing operation to accumulate sequencing signals from the cells, normalizing those sequencing signals, and determining bases based on the normalized sequencing signals.Type: GrantFiled: October 21, 2020Date of Patent: February 6, 2024Assignee: Roche Sequencing Solutions, Inc.Inventors: Morgan Mager, John Mannion
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Patent number: 11891657Abstract: A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.Type: GrantFiled: November 9, 2020Date of Patent: February 6, 2024Assignee: Orflo Technologies LLCInventors: Harold E. Ayliffe, Donald O'Neil