Patents Examined by Nathan Bowers
  • Patent number: 10287543
    Abstract: The invention is directed to a Perfusion device for biological tissues comprising a casing having two parts, a first part (1) and a second part (9), a holder (7) for a plurality of hollow penetration structures (8), wherein the hollow penetration structures (8) are provided with at least one orifice having fluid communication through the holder (7) a support (5) for the biological tissue (6) characterized in that the support (5) for the biological tissue (6) is positioned in the casing at a distance to the holder (7) that by joining the first part (1) and the second part (9) to form the casing, the hollow penetration structures (8) are in proximity to the holder (7). Use of the perfusion device in a process for disaggregation of a biological tissue to yield target cells.
    Type: Grant
    Filed: October 25, 2016
    Date of Patent: May 14, 2019
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Carsten Poggel, Andreas Bosio, Wolfgang Stoters, Timo Adams
  • Patent number: 10281369
    Abstract: Disclosed are methods, systems, and devices for detecting biological analytes in a sample. The disclosed technology can be used to obtain readings of analyte concentration in a sample by imaging scattered light from an angled narrow beam illuminator. A fluid sample containing one or more biological, organic, and inorganic analytes including proteins, viruses, bacteria, phages, toxins, peptides, DNA, RNA, hormones, chemicals, drugs, and isotopes can be transferred to a microfluidic device having one or more channels with dimensions to generate capillary action for sample transport. The geometry of the microfluidic device may include a reservoir and sensing area, wherein an immunometric reaction can take place for the narrow beam scanning. The test particle may be coated with a specific binding member that is used to bind the binding pair member on an analyte in a sample. Test particles form the binding and the particle/analyte conjugate may be scanned.
    Type: Grant
    Filed: December 23, 2016
    Date of Patent: May 7, 2019
    Assignee: Vor, Inc.
    Inventors: TsungFeng Wu, Yu-Chen Chen
  • Patent number: 10258590
    Abstract: Embodiments of the disclosure are drawn to an enteral feeding device for hydrolyzing triglycerides in a nutritional formula. The device may include a body housing a chamber, an inlet configured to fluidly couple with a source of nutritional formula, and an outlet configured to fluidly couple with an enteral feeding tube. The device may include a headspace and a plurality of particles contained within the chamber, wherein the lipase is covalently bonded to the plurality of particles. The device may include an inlet filter located between the inlet and the chamber, wherein the inlet filter contains a first plurality of openings, and an outlet filter located between the chamber and the outlet, wherein the outlet filter has a second plurality of openings smaller than the plurality of particles.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: April 16, 2019
    Assignee: Alcresta Therapeutics, Inc.
    Inventors: Robert Gallotto, Greta L. Loring, Kenneth Gary, Edward S. Park, David J. Brown, Willem Robert Klaas Schoevaart, Michiel Christian Alexander van Vliet
  • Patent number: 10253316
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: June 30, 2018
    Date of Patent: April 9, 2019
    Assignee: INSCRIPTA, INC.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Patent number: 10247724
    Abstract: A Petri dish bioreactor can allow a standard Petri dish to be converted to a microscopically observable bioreactor. A bioreactor can be formed by the compression of a device against the edge of a Petri dish thus creating a sealed isolated chamber. One advantage of this conversion is to provide an inexpensive device that can be used for cell culture, observation, transportation, and storage in the laboratory and clinical setting. Further, by creating a sealed bioreactor chamber accessible by ports, the device can reduce cross contamination and allow for the maintenance of a controlled microenvironment within the bioreactor.
    Type: Grant
    Filed: May 4, 2018
    Date of Patent: April 2, 2019
    Assignee: Autobiologic Inc.
    Inventors: An Vuong, Charlie Chandsawangbhuwana
  • Patent number: 10246730
    Abstract: A method includes disposing a solution including a microbe or a virion on a surface of a semiconductor substrate, the semiconductor substrate having a trench extending from the surface to a region within the semiconductor substrate; wherein the the microbe or the virion is trapped within the trench of the semiconductor substrate.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 2, 2019
    Inventors: Yann Astier, David Esteban, Judson R. Holt, Henry K. Utomo
  • Patent number: 10239060
    Abstract: A system and method of manufacture for the system, comprising a set of heater-sensor dies, each heater-sensor die comprising an assembly including a first insulating layer, a heating region comprising an adhesion material layer coupled to the first insulating layer and a noble material layer, and a second insulating layer coupled to the heating region and to the first insulating layer through a pattern of voids in the heating region, wherein the pattern of voids in heating region defines a coarse pattern associated with a heating element of the heating region and a fine pattern, integrated into the coarse pattern and associated with a sensing element of the heating region; an electronics substrate configured to couple heating elements and sensing elements of the set of heater-sensor dies to a controller; and a set of elastic elements configured to bias each of the set of heater-sensor dies against a detection chamber.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: March 26, 2019
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Sundaresh Brahmasandra, Thomas Haddock, Patrick Duffy, Jeffrey Williams
  • Patent number: 10240143
    Abstract: There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: March 26, 2019
    Assignee: BIO-FD&C CO., LTD
    Inventors: Sang-Hyun Moh, Jeong-Hun Lee, Hyo-Hyun Seo, Dong-Sun Shin, Moon-Jin Cho, Hyo-Seok Kang, Hae-Soo Jung, Hyun-Jin Oh, Yeong-Wook Yu, Ji-Hong Moh, Mun-Seong Heo, Ser-Jong Yoo, Hyun-E Lee, Jin-Hyeong Lee, Yu-Ri Lee, Eun-Ae Kim, Seung-Jun Lee
  • Patent number: 10241054
    Abstract: A system for use in rapid sample analysis that includes a biosensor reagent, wherein the biosensor reagent includes living biological cells; a reservoir card, wherein the reservoir card stores the biosensor reagent; and a test cartridge base, wherein the test cartridge base is configured to accept the reservoir card, and wherein the test cartridge base further includes a contoured reaction chamber; and an inlet channel connected to and entering the reaction chamber at a predetermined angle thereto.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: March 26, 2019
    Assignee: Fundamental Solutions Corporation
    Inventors: Marvin R. Williams, Charles McBrairty, Daniel W. Pfautz, Thomas J. Zupancic, Lingchun Zeng, Andrew Weiman, Richard S. Brody, Joseph D. Kittle, Anthony Truscott, Robert Baranowski
  • Patent number: 10226771
    Abstract: A system for thermocycling biological samples within detection chambers comprising: a set of heater-sensor dies, each heater-sensor die comprising a heating surface configured to interface with a detection chamber and a second surface, inferior to the heating surface, including a first connection point; an electronics substrate, comprising a first substrate surface coupled to the second surface of each heater-sensor die, an aperture providing access through the electronics substrate to at least one heater-sensor die, and a second substrate surface inferior to the first substrate surface, wherein the electronics substrate comprises a set of substrate connection points at least at one of the first substrate surface, an aperture surface defined within the aperture, and the second substrate surface, and wherein the electronics substrate is configured to couple heating elements and sensing elements of the set of heater-sensor dies to a controller; and a set of wire bonds, including a wire bond coupled between the
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: March 12, 2019
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Sundaresh Brahmasandra, Thomas Haddock, Patrick Duffy, Jeffrey Williams
  • Patent number: 10226773
    Abstract: A micro heating device according to an embodiment of the inventive concept comprises a support part having at least one or more heating part, an oil chamber positioned over the support part and filled with oil therein, a specimen chamber having a reaction space into which a specimen is loaded and which is provided so as to be dipped into the oil, and a drive part configured to move the specimen chamber in the oil. The specimen chamber includes a temperature sensor for measuring a temperature of the specimen chamber.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: March 12, 2019
    Inventors: Dae-Sik Lee, Moon Youn Jung
  • Patent number: 10227556
    Abstract: Cell culture devices and methods of their use are provided according to aspects of the present invention which include a base having a pair of spaced apart wells and at least one fluid port defined from an exterior of the base to each of the wells, each fluid port being spaced above the base sheet by a distance sufficient to introduce a material above a matrix deposited on the base sheet in the wells; and a cover having a pair of spaced apart well covering portions that are each disposed above a respective one of the one spaced apart wells of the base body when the cover is covering the upper surface of the base body and having a gas passage defined between the spaced apart well covering portions and at least one gas port defined from an exterior of the cover to each of the well covering portions.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: March 12, 2019
    Assignee: Wayne State University
    Inventors: Bonnie F. Sloane, Yong Xu, Kyungmin Ji, Hongen Tu, Kamiar Moin, Kingsley Osuala
  • Patent number: 10221391
    Abstract: A closed system suitable for the aseptic culturing of therapeutic cells. The present system facilitates the aseptic manufacturing of cells for use in therapy, without the need for a clean-room environment because no open processing steps are required.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: March 5, 2019
    Inventors: Rainer Ludwig Knaus, Gavin Holms, Katy Rebecca Newton, Tina Lesley Crombie, Andrew Roy MacLeod Provan
  • Patent number: 10214764
    Abstract: The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: February 26, 2019
    Assignee: BIOMERIEUX, INC.
    Inventors: John Walsh, Jones M. Hyman, Christopher Ronsick, John Link, Ron Robinson, Mark Wilson
  • Patent number: 10214715
    Abstract: A bioreactor system for stem cell expansion has a fluid-filled reactor chamber in which stem cells are cultured. The culture compartment of the reactor chamber is transversed by a perfusion core with multiple niche-like scaffolds positioned along the length of the perfusion core. The bioreactor system also uses a media gas exchange module that is free standing and does not require its inclusion in a CO2 incubator.
    Type: Grant
    Filed: March 5, 2015
    Date of Patent: February 26, 2019
    Assignee: Synthecon, Inc.
    Inventor: Stephen S. Navran, Jr.
  • Patent number: 10214458
    Abstract: This invention relates to processes and systems for converting fresh food waste into nutrient rich hydrolysates and particulate compositions. The invention also relates to the hydrolysates and compositions useful, for example, as fertilizers, feedstock or other nutrient supplements.
    Type: Grant
    Filed: December 13, 2016
    Date of Patent: February 26, 2019
    Inventors: Daniel M. Morash, Mark LeJeune
  • Patent number: 10209259
    Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: February 19, 2019
    Inventors: Magdalena Anna Ostrowski, Rudolf Grimm, Kevin P. Killeen, Karla M. Robotti
  • Patent number: 10209243
    Abstract: Disclosed is a device (100) for cardiac electrophysiology screening comprising a substrate (10) comprising a cavity (42), said substrate carrying a deformable layer (32) extending over said cavity (42), wherein a portion of said deformable layer (32) covers said cavity and acts as a membrane over said cavity (32), said portion having a surface comprising a pattern of grooves (44) and carrying a multi-electrode structure (110, 110?); and a plurality of cardiomyocytes (130) assembled in at least some of said grooves (44). A method of manufacturing such a device (100) is also disclosed.
    Type: Grant
    Filed: July 13, 2011
    Date of Patent: February 19, 2019
    Assignee: Koninklijke Philips N.V.
    Inventors: Ronald Dekker, Anja Van De Stolpe, Berent Jan Van Meer, Saeed Khoshefetrat Pakazad, Angel Savov
  • Patent number: 10208279
    Abstract: The present invention relates to the field of testing and evaluation of biological samples and provides an apparatus for cultured samples including at least one independently accessible module adapted for incubating at least one of a number of samples wherein the at least one module is operatively associated with a light source and a movable optical inspection means adapted for motion about a viewing axis through the module to enable a sweeping of viewing area. The invention also provides a method of assessing cultured samples for viability, including the steps of: disposing biological samples in a substantially elliptical arrangement within a culturing chamber of an independently accessible module and imaging individual samples of the substantially elliptical arrangement with optical inspection means driven within an X-Y plane that is normal to a viewing axis through the module to obtain time lapse measurement of development of individual samples.
    Type: Grant
    Filed: March 3, 2014
    Date of Patent: February 19, 2019
    Assignee: Genea IP Holdings Pty Limited
    Inventors: Eduardo Vom, Simon Davies, Adrian Higgins, Jasmine Pouladi, Ben Stewart-Steele, Simon Spence
  • Patent number: 10202572
    Abstract: A culture container includes a first transparent member being capable of keeping a predetermined temperature; a second transparent member facing to the first transparent member; a housing member to which the first transparent member and the second transparent member are adhered forming a culture space being capable of housing a well plate together with the first transparent member and the second transparent member; and a sealing member for sealing a liquid injected into the culture space between the first transparent member and the housing member.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: February 12, 2019
    Assignee: Sony Corporation
    Inventors: Eiichi Tanaka, Shinsuke Haga, Shin Hasegawa, Tatsuya Minakawa, Suguru Dowaki, Eriko Matsui