Abstract: Nucleic acid sequences encoding the Phaseolus vulgaris storage protein arcelin-1 are provided. These sequences are useful in providing transformed plant cells and plants which are insect-resistant.
Abstract: A process is disclosed for enzymatically converting lower alkyl alcohols to corresponding aldehydes and hydrogen peroxide in the presence of oxygen under process conditions which increase the catalytic capacity of alcohol oxidase enzymes. Such process conditions involve low temperatures, high substrate concentrations and an enriched supply of oxygen. Enzymes may be used in the form of whole cells, a soluble cell free extract or a highly purified fraction, and the process may be employed in batch or continuous operation.
Abstract: Methods and apparatus for the introduction of foreign substances, such as recombinant DNA, into living cell interiors is disclosed. The desired substance to be introduced is dissolved or suspended in water or another suitable liquid to form a solution which is then nebulized and frozen to produce ice particles. These ice particles are accelerated toward and impact upon a target tissue where some of the particles impregnate at least some cells in the target tissue without killing the cells. Following impregnation, the ice particles melt, leaving behind the desired substance in the protoplasm of the bombarded cell.
Type:
Grant
Filed:
March 31, 1992
Date of Patent:
June 15, 1993
Inventors:
Chris Brinegar, James Gilmore, Michael Johnson, Nigel Walker