Abstract: Methods for obtaining N-terminal methionine-free proteins involve a novel E. coli methionine amino peptidase. The method is capable of in vitro or in vivo application. For in vivo application, a plasmid-borne DNA encoding the peptide is expressed in a bacterial host.
Type:
Grant
Filed:
May 6, 1986
Date of Patent:
September 12, 1989
Assignee:
Cetus Corporation
Inventors:
Arie Ben-Bassat, Keith A. Bauer, Shing Chang, Sheng-Yung Chang