Patents Examined by Paul J Holland
  • Patent number: 11142783
    Abstract: The invention relates to an optimized seed train expansion process.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: October 12, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Andre De Castro Bizarro Duarte Canelas, Wouter Adrianus Van Winden
  • Patent number: 11130970
    Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
    Type: Grant
    Filed: February 18, 2021
    Date of Patent: September 28, 2021
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Ryan T. Gill, Tanya Elizabeth Warnecke Lipscomb
  • Patent number: 11124478
    Abstract: The present invention is directed to a process for preparing a compound of formula I-11 through multiple-step reactions:
    Type: Grant
    Filed: February 20, 2020
    Date of Patent: September 21, 2021
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: John Y. L. Chung, Kevin Campos, Edward Cleator, Robert F. Dunn, Andrew Gibson, R. Scott Hoerrner, Stephen Keen, Dave Lieberman, Zhuqing Liu, Joseph Lynch, Kevin M. Maloney, Feng Xu, Nobuyoshi Yasuda, Naoki Yoshikawa, Yong-Li Zhong
  • Patent number: 11124813
    Abstract: The present invention relates to a compound of general formula I The present invention also relates to a method of producing N-acetyl homoserine and/or derivatives thereof, the method comprising contacting at least one recombinant cell in an aqueous medium with acetate wherein the recombinant cell comprises an increased activity relative to a wild type cell of (a) an enzyme E1, a homoserine dehydrogenase (EC1.1.1.3) and/or an enzyme E5, an aspartokinase (EC2.7.2.4); and (b) an enzyme E2, a homoserine O-acetyl transferase (EC2.3.1.31) and the acetate is maintained at a concentration of at least about 0.001 g/L in the aqueous medium.
    Type: Grant
    Filed: July 26, 2017
    Date of Patent: September 21, 2021
    Assignee: Evonik Operations GmbH
    Inventors: Thomas Haas, Steffen Schaffer, Thomas B├╝lter, Simon Beck
  • Patent number: 11118205
    Abstract: The present invention discloses a process for producing N-acetyl-D-glucosamine and D-glucosamine salts by microbial fermentation. The invention includes a method to produce N-acetyl-D-glucosamine and/or D-glucosamine salts with higher efficiency and higher yield by increasing the effect of N-acetyl-D-aminomannose-6-phosphate epimerase in microorganisms.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: September 14, 2021
    Inventor: Lan Sun
  • Patent number: 11110128
    Abstract: The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.
    Type: Grant
    Filed: September 10, 2020
    Date of Patent: September 7, 2021
    Assignee: RegenLab USA LLC
    Inventors: Antoine Turzi, Donald Du Toit
  • Patent number: 11099176
    Abstract: The present subject matter provides lactate biosensors as well as compositions, devices, and methods comprising such biosensors.
    Type: Grant
    Filed: November 19, 2016
    Date of Patent: August 24, 2021
    Assignee: Duke University
    Inventors: Homme W. Hellinga, Malin J. Allert
  • Patent number: 11096966
    Abstract: The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: August 24, 2021
    Assignee: RegenLab USA LLC
    Inventors: Antoine Turzi, Donald Du Toit
  • Patent number: 11098289
    Abstract: Described are improved uricase sequences having beneficial effects and methods of treating patients suffering from hyperuricemia.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: August 24, 2021
    Assignee: MedImmune, LLC
    Inventors: Manuel Baca, Andrew C. Nyborg
  • Patent number: 11078499
    Abstract: The present invention provides for a genetically modified host cell comprising a first polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase, and having an enzymatic activity to decarboxylate a phenylacetic acid into a toluene and a carbon dioxide, and a second polypeptide comprising a sequence having at least 70% amino acid sequence identity with a phenylacetate decarboxylase activating enzyme, and having an enzymatic activity to cleave a S-adenosylmethionine (SAM) to form a methionine and a 5?-deoxyadenosyl radical.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: August 3, 2021
    Assignee: The Regents of the University of California
    Inventors: Harry R. Beller, Andria V. Rodrigues, Kamrun Zargar
  • Patent number: 11072663
    Abstract: Cysteine engineered monospecific and bispecific EGFR and/or c-Met FN3 domain containing molecules comprising one or more free cysteine amino acids are prepared by mutagenizing a nucleic acid sequence of a parent molecule and replacing one or more amino acid residues by cysteine to encode the cysteine engineered FN3 domain containing monospecific or bispecific molecules; expressing the cysteine engineered FN3 domain containing molecules; and recovering the cysteine engineered FN3 domain containing molecule. Isolated cysteine engineered monospecific or bispecific FN3 domain containing molecules may be covalently attached to a detection label or a drug moiety and used therapeutically.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: July 27, 2021
    Assignee: Janssen Biotech, Inc.
    Inventors: Shalom Goldberg, Steven Jacobs, Tricia Lin, Karyn O'Neil
  • Patent number: 11060108
    Abstract: A method for stably achieving high expression of a foreign gene in mammalian cells using a novel DNA element is disclosed. More specifically, the present application discloses a DNA element which enhances the activation of transcription by changing the chromatin structure around a gene locus into which a foreign gene expression unit has been introduced.
    Type: Grant
    Filed: July 26, 2019
    Date of Patent: July 13, 2021
    Assignee: Daiichi Sankyo Company, Limited
    Inventors: Daisuke Nishimiya, Tatsuya Inoue
  • Patent number: 11060079
    Abstract: The present disclosure relates to biosynthetic pathways for producing flavor and fragrance chemicals, such as green notes including trans-2-unsaturated aldehydes and lactones. The present disclosure provides methods for producing trans-2-unsaturated aldehydes, delta-lactones, and gamma-lactones. The present disclosure provides pathways for the preparation of trans-2-unsaturated aldehydes, delta-lactones, and gamma-lactones by reacting aldehydes in the presence of aldolases.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: July 13, 2021
    Assignee: ARDRA INC.
    Inventors: Pratish Gawand, Jonas Ernst Norbert Muller
  • Patent number: 11046982
    Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.
    Type: Grant
    Filed: February 6, 2020
    Date of Patent: June 29, 2021
    Assignee: Codexis, Inc.
    Inventors: Lynne Gilson, Steven J. Collier, Joly Sukumaran, Wan Lin Yeo, Oscar Alvizo, Ee Ling Teo, Robert J. Wilson, Junye Xu
  • Patent number: 11035003
    Abstract: Focal and segmental glomerulosclerosis (FSGS) is a kidney disorder of unknown etiology and up to 20% of patients on dialysis have this diagnosis. A large family with hereditary FSGS carries a missense mutation in the TRPC6 gene on chromosome 11q, encoding the ion channel protein Transient Receptor Potential Cation Channel 6. The missense mutation is a P112Q substitution, which occurs in a highly conserved region of the protein, enhances TRPC6-mediated calcium signals in response to agonists such as angiotensin II, and alters the intracellular distribution of TRPC6 protein. Previous work has emphasized the importance of cytoskeletal and structural proteins in proteinuric kidney diseases. Our findings suggest a novel mechanism for glomerular disease pathogenesis.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: June 15, 2021
    Assignee: DUKE UNIVERSITY
    Inventors: Michelle Winn, Margaret A. Pericak-Vance, Jeffery M. Vance
  • Patent number: 11034939
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: June 15, 2021
    Assignee: Codexis, Inc.
    Inventors: Weng Lin Tang, Helen Hsieh, Son Pham, Derek Smith, Steven J. Collier
  • Patent number: 11028346
    Abstract: The present invention relates to detergent compositions comprising protease variants and alpha-amylases or variants thereof. Furthermore, the present invention relates to methods of using the detergent compositions.
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: June 8, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Carsten Andersen, Yanfei Wang, Wenwen Tao, Bitten Plesner, Elena Genesca Pont
  • Patent number: 11021720
    Abstract: A process for producing ethanol includes steps of pre-treatment of the lignocellulosic vegetable raw material, including the steps consisting in destructuring the lignocellulosic vegetable raw material, then in separating, on the one hand, the cellulose (C6) capable of then being hydrolysed (and fermented for the production of ethanol) and, on the other hand, the hemicelluloses capable of then being hydrolysed and the lignins. The hydrolysis of the cellulose and of the hemicelluloses is then carried out in a sequenced manner according to the following steps consisting in: i) beginning the enzymatic hydrolysis of the cellulose by at least one enzyme for a first period with a view to obtaining an intermediate hydrolysate; ii) adding hemicelluloses to the intermediate hydrolysate; iii) continuing the enzymatic hydrolysis of the mixture until a final hydrolysate is obtained at the end of a total period of enzymatic hydrolysis.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: June 1, 2021
    Assignee: COMPAGNIE INDUSTRIELLE DE LA MATIERE VEGETALE
    Inventors: Bouchra Benjelloun Mlayah, Michel Delmas
  • Patent number: 11016097
    Abstract: The present invention provides compositions comprising chimeric polypeptides that bind to free ubiquitin proteins or free ubiquitin-like proteins with high affinity, as well as chimeric polypeptides that bind to both free and conjugated ubiquitin proteins or free and conjugated ubiquitin-like proteins, and methods of using the chimeric polypeptides to determine the amount of free or total ubiquitin or free or total ubiquitin-like proteins in various types of samples.
    Type: Grant
    Filed: January 18, 2019
    Date of Patent: May 25, 2021
    Assignee: Colorado State University Research Foundation
    Inventors: Robert E. Cohen, Yun-Seok Choi
  • Patent number: 11001825
    Abstract: The present disclosure discloses a thermophilic L-asparaginase mutant and screening and fermentation methods thereof, and belongs to the field of gene engineering, enzyme engineering and fermentation engineering. In Bacillus subtilis 168, a Pyrococcus yayanosii CH1-derived L-asparaginase encoding gene is used as a template, and a mutation library is constructed by an error-prone PCR (epPCR) technology. A mutant strain with improved specific enzyme activity is screened through a high-flux screening method of synchronous cell disruption and enzyme activity measurement. Mutated residues included in a positive mutant are analyzed to construct a composite mutant strain S17G/A90S/R156S/K272A with improved specific enzyme activity and specific enzyme activity of 3108 U/mg. An expression quantity of the composite mutant strain in the Bacillus subtilis 168 is increased through measures of a strong promoter P43 and RBS optimization.
    Type: Grant
    Filed: January 24, 2019
    Date of Patent: May 11, 2021
    Assignee: Jiangnan University
    Inventors: Zhiming Rao, Xu Li, Xian Zhang, Shuqin Xu, Jingyi Hu, Meijuan Xu, Taowei Yang