Patents Examined by Paul J Holland
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Patent number: 11603522Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.Type: GrantFiled: December 16, 2019Date of Patent: March 14, 2023Assignees: NOVOZYMES A/S, NOVOZYMES NORTH AMERICA, INC.Inventors: Carsten Andersen, Randall Deinhammer, Thomas Agersten Poulsen, Miguel Duarte Toscano, Peter Kamp Hansen, Henrik Friis-Madsen, Anders Viksoe-Nielsen, Signe Eskildsen Larsen, Lars L. H. Christensen
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Patent number: 11597959Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.Type: GrantFiled: December 14, 2020Date of Patent: March 7, 2023Assignee: Amgen Inc.Inventors: Chung-Jr Huang, Xiaoming Yang
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Patent number: 11597917Abstract: The present invention is related to a dual promoter lentiviral vector and methods of use for the treatment of diseases and disorders, specifically lysosomal storage disorders.Type: GrantFiled: June 6, 2018Date of Patent: March 7, 2023Assignees: The Medical College of Wisconsin, Inc., University Health Network, Washington University in St. LouisInventors: Jeffrey A. Medin, John F. Dipersio, Murtaza S. Nagree
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Patent number: 11578342Abstract: The disclosure discloses recombinant Bacillus subtilis for synthesizing guanosine diphosphate fucose and a construction method and application thereof. The recombinant Bacillus subtilis is obtained by intensively expressing guanylate kinase and nucleotide diphosphokinase genes and expressing exogenous fucokinase and phosphate guanylyltransferase genes in a genome of Bacillus subtilis 168. According to the disclosure, a bacterial strain for synthesizing the guanosine diphosphate fucose is obtained by reconstructing the Bacillus subtilis 168, with a volume of intracellular accumulation up to 196.15 g/L. According to the disclosure, by intensively expressing the guanylate kinase and nucleotide diphosphokinase genes, and enhancing the supply of intracellular GDP-L-fucose composition cofactors, the synthesis of the guanosine diphosphate fucose is promoted. The construction method for the recombinant Bacillus subtilis of the disclosure is simple and convenient to use, thus having good application prospects.Type: GrantFiled: January 20, 2020Date of Patent: February 14, 2023Assignees: BRIGHT DAIRY & FOOD CO., LTD., Jiangnan UniversityInventors: Long Liu, Jian Chen, Guocheng Du, Jieying Deng, Chunmei Chen, Xueqin Lv, Jianghua Li
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Patent number: 11572552Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.Type: GrantFiled: December 18, 2020Date of Patent: February 7, 2023Assignee: HOFFMANN-LA ROCHE INC.Inventors: Mara Boenitz-Dulat, Martin Schatte
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Patent number: 11555204Abstract: The present invention provides an approach to enhancing the production of a foreign protein serving as a protein-based pharmaceutical product in host cells such as cultured cells derived from a mammal. The present invention provides transformed cells having a novel Hspa5 gene promoter, and a method for secreting and producing a foreign protein at high levels using the transformed host cells.Type: GrantFiled: October 2, 2017Date of Patent: January 17, 2023Assignee: Daiichi Sankyo Company, LimitedInventors: Kenji Masuda, Koichi Nonaka, Hiroki Tanemura
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Patent number: 11542509Abstract: Provided herein are systems, compositions, and methods for the incorporation of unnatural amino acids into proteins via nonsense suppression or rare codon suppression. Nonsense codons and rare codons may be introduced into the coding sequence of a protein of interest using a CRISPR/Cas9-based nucleobase editor described herein. The nucleobase editors are able to be programmed by guide nucleotide sequences to edit the target codons in the coding sequence of the protein of interest. Also provided are application enabled by the technology described herein.Type: GrantFiled: August 24, 2017Date of Patent: January 3, 2023Assignee: President and Fellows of Harvard CollegeInventors: Juan Pablo Maianti, David R. Liu, Luke W. Koblan
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Patent number: 11523623Abstract: The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of, e.g., chlorophyll and may thus provide lightening the color of the protein-enriched extracts. The methods generally include treatment with peracetic acid or hydrogen peroxide and filtrations. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant material.Type: GrantFiled: January 16, 2020Date of Patent: December 13, 2022Assignee: R.J. Reynolds Tobacco CompanyInventors: Nicholas Partain, Joshua D. Morton, Barry Bratcher, John-Paul Mua, Kyle Ford
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Patent number: 11525124Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.Type: GrantFiled: May 11, 2020Date of Patent: December 13, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Helen Franklin, Cynthia Cech, Timothy Kellogg Craig, Aruna Ayer, Kirti Dhiman, Natalie B. Chechelski Johnston, Joshua N. Mabry, Arkadiusz Bibillo, Peter Crisalli, Randall W. Davis
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Patent number: 11512305Abstract: A nanoparticle (for example, quantum dot) serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes.Type: GrantFiled: December 13, 2017Date of Patent: November 29, 2022Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Igor L. Medintz, James N. Vranish, Mario Ancona, Kimihiro Susumu, Sebastian A. Diaz
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Patent number: 11512336Abstract: Described herein is a method for producing a transgenic cell product wherein the gene of interest is operably linked to an inducible promoter other than AOX1. Production of the transgenic cell product is activated when the host cell is grown on a non-repressing carbon source for de-repressing the inducible promoter and an amount of an inducer compound selected from the group consisting of: formaldehyde; S-formylglutathione; S-hydroxymethyl glutathione; formic acid; an alkali metal salt of formic acid; and an alkaline earth metal salt of formic acid; sufficient to induce the inducible promoter is added to the host cell culture.Type: GrantFiled: September 17, 2021Date of Patent: November 29, 2022Assignee: BioBoost Synbio Consulting Inc.Inventors: Oleg Tyurin, Mingyang Sun
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Patent number: 11505791Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.Type: GrantFiled: December 2, 2019Date of Patent: November 22, 2022Assignee: The Regents of the University of CaliforniaInventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
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Patent number: 11492389Abstract: The present invention relates to cell culture media supplements or complete media compositions comprising plant-produced heterologous recombinant human albumin, as well as methods of making the cell culture media, and methods of using the supplemented cell culture media to improve viability, productivity, and growth characteristics of cultured cells.Type: GrantFiled: June 7, 2022Date of Patent: November 8, 2022Assignee: Ventria Biosciences Inc.Inventors: Steven Clyde Pettit, Mary Ann Michelle Fernandez Santos, Ning Huang
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Patent number: 11492614Abstract: The present disclosure relates to a novel CRISPR-Cas9 based system for editing mitochondrial DNA. Aspects of the disclosure provide for mitochondrial translocation of both the guide RNA and the recombinant Cas9 nuclease.Type: GrantFiled: November 15, 2017Date of Patent: November 8, 2022Assignee: Research Institute at Nationwide Children's HospitalInventors: Kenneth John McLaughlin, Syed-Rehan Ashfaq Hussain
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Patent number: 11485979Abstract: One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus Komagataella. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.Type: GrantFiled: September 25, 2020Date of Patent: November 1, 2022Assignees: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, KANEKA CORPORATIONInventors: Yoichiro Ito, Jun Ishii, Yasuyuki Nakamura, Akihiko Kondo, Teruyuki Nishi
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Patent number: 11453896Abstract: The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one ?-ketothiolase enzyme having thiolysis activity for ?-keto-(C6) acyl-CoA (i.e., ?-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.Type: GrantFiled: July 13, 2020Date of Patent: September 27, 2022Assignee: KANEKA CORPORATIONInventors: Hisashi Arikawa, Shunsuke Sato
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Patent number: 11434493Abstract: The invention provides important tools for the use of Chlorophyte organisms, including regulatory sequences useful for the expression of transgenes in such organisms. Also provided are vectors and expression cassettes containing promoters and/or terminators disclosed herein for expression of transgenes in Chlorophyte organisms. Methods of using these tools are also provided.Type: GrantFiled: February 4, 2021Date of Patent: September 6, 2022Assignee: Viridos, Inc.Inventors: John Verruto, Moena Aqui
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Patent number: 11434514Abstract: The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.Type: GrantFiled: November 22, 2021Date of Patent: September 6, 2022Assignee: Amgen Inc.Inventors: Chung-Jr Huang, Xiaoming Yang
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Patent number: 11421251Abstract: Disclosed herein are Type I Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system related compositions and methods of using said Type I CRISPR/Cas system related compositions for altering gene expression and genome engineering. The invention relates to compositions comprising Type I CRISPR-Cas polypeptides and CRISPR array nucleic acids designed for genome modification in eukaryotic cells and for targeted killing of eukaryotic cells.Type: GrantFiled: April 1, 2020Date of Patent: August 23, 2022Assignees: Duke University, North Carolina State UniversityInventors: Charles A. Gersbach, Adrian Pickar Oliver, Chase Beisel
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Patent number: 11408012Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.Type: GrantFiled: January 29, 2022Date of Patent: August 9, 2022Assignee: Inscripta, Inc.Inventors: Andrew Garst, Ryan T. Gill, Tanya Elizabeth Warnecke Lipscomb