Abstract: The present invention relates to a clearing technique which uses a solution containing at least one of a compound having a delipidation ability, a compound having a biochrome decoloring ability, a compound having a decalcification ability, a compound having a refractive index adjusting ability, and a compound having a tissue swelling ability. The clearing technique is suitable for use in high-throughput and low-magnification imaging which involves a simple process.

Abstract: Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer defining a channel, a set of wells, and a cavity. A second layer may be coupled to the first layer. The second layer may include a protrusion extending towards the first layer. The second layer may define an opening configured to receive a fluid. The channel may establish a fluid communication path between the opening and the set of wells. A container may be slidable within the cavity during use. The protrusion may be configured to penetrate a wall of the container.

Abstract: The object of the invention is to provide an automatic analysis apparatus which is capable of both sterilizing a reagent and suppressing property variations in the reagent. Provided is an automatic analysis apparatus including a reagent vessel which holds a reagent; a suction nozzle which sucks the reagent; an analysis unit which executes an analysis operation by adding a reagent sucked from the reagent vessel to a specimen via the suction nozzle; an ultraviolet ray source which sterilizes a reagent by ultraviolet irradiation; and an electrode or a substrate which supplies electric power to the ultraviolet ray source, in which a heat insulation portion is arranged between a reagent in the suction nozzle and the ultraviolet ray source and the electrode or the substrate, or, it is isolated between a reagent in the suction nozzle and the ultraviolet ray source and the electrode or the substrate.

Abstract: An analysis method is a method of analyzing a second substance in a sample including the second substance produced by decomposition of a first substance, and includes analyzing a sample and a compound having a known concentration and detecting the first substance, the second substance and the above-mentioned compound, calculating an intensity or a concentration of the first substance obtained from the above-mentioned data based on data obtained by the detection and a relative response factor in regard to the first substance and the above-mentioned compound, and producing information about an amount or a concentration of the second substance excluding the second substance produced from the first substance in the analysis, based on an intensity or a concentration of the first substance.

Abstract: A detection method includes acquiring at least one first relative response factor obtained by measurement of a sample having a same component as at least one component included in a subject sample at a known concentration and a sample having at least one compound at a known concentration, or at least one first threshold value based on each of the at least one first relative response factor, measuring the subject sample and a sample including the at least one compound, and calculating at least one second relative response factor in regard to at least one component of the subject sample and the at least one compound, and producing first information in regard to a change in concentration of the subject sample based on the at least one first relative response factor or the at least one first threshold value, and the corresponding at least one second relative response factor.

Abstract: A microchip includes a body having an upper surface and a lower surface and a plurality of inner channels formed between the upper surface and the lower surface in which the plurality of inner channels include an input channel configured to receive an assay sample including target antigens and injected through a though hole, a reaction channel configured to be in fluid communication with the input channel and including magnetic particle-first antibody complexes to be subjected to a first antigen-antibody reaction with the target antigens, the magnetic particle-first antibody complexes including magnetic particles and first antibodies, and a detection channel configured to be in fluid communication with the reaction channel and including second antibodies to be subjected to a second antigen-antibody reaction with the immune complexes flowed from the reaction channel.

Abstract: The present invention provides compositions and methods that allow lipid membranes to be imaged optically at nanoscale resolution via a lipid-optimized form of expansion microscopy, also referred to as membrane expansion microscopy (mExM). mExM, via a post-expansion antibody labeling protocol, enables protein-lipid relationships to be imaged in organelles such as mitochondria, the endoplasmic reticulum, the nuclear membrane, and the Golgi apparatus. mExM may be of use in a variety of biological contexts, including the study of cell-cell interactions, intracellular transport, and neural connectomics.

Abstract: A microfluidic chip comprises: a first unit which has a channel for a cell sample to pass through and is configured to separate circulating tumor cells in the cell sample; a second unit, a front end of which communicates with a tail end of the first unit, and the second unit is configured to capture single cells from the separated circulating tumor cells and subject the captured single cells to closed lysis; and a gel layer which is provided at the second unit. The microfluidic chip is configured to implement the binding of a protein molecule of the single cell with an antibody in the gel layer after the single cell is lysed. A cell separation and western blotting method using the microfluidic chip comprises: lysing circulating tumor cells, capturing, and implementing the binding of a lysate with an antibody.

Abstract: An isotopic ratio outlier analysis (IROA) standard sample of metabolite compounds is disclosed. Each of whose compounds has a molecular weight of 2000 AMU or less, and is present as first and second isotopomers that are equally present at two predetermined isotopomeric balances, and contain 2 to 10% of a first isotope, and 90 to 98% of a second isotope, respectively. An internal standard is also disclosed that contains the same metabolite compounds as the IROA standard sample, but contains only compounds containing the heavier of the two isotopes. Methods of making and using the above and related materials are also disclosed.

Abstract: A method for preparing an analysis sample from a sample containing a glycan, includes: performing esterification reaction that subjects at least a part of a sialic acid included in the glycan to esterification other than lactonization; and performing amidation reaction that converts an esterified form of a sialic acid modified through the esterification into an amidated form through contacting the sample with an amidation reaction solution containing at least one compound selected from the group consisting of ammonia, amines, hydrazine, hydrazine derivatives, and hydroxyamine, and salts thereof to be reacted with the sialic acid modified through the esterification.

Abstract: A method for preparing droplets using a microfluidic chip system is provided. The microfluidic chip system includes a droplet generation device, a power generation device, a collection bottle, a connection device, and a preparation platform, the droplet generation device includes a chip body, the chip body defines a continuous phase inlet for receiving a continuous phase and a dispersed phase inlet for receiving a dispersed phase. The power generation device is activated to form a pressure difference among the collection bottle, the connection device, and the chip body, wherein the pressure difference promotes the dispersed phase and the continuous phase to converge and flow into the collection bottle in form of the droplets.

Abstract: A probe for collecting optically stimulated electron emission to inspect chemical reactions of a surface includes a light source to emit light on the surface, a collector, and a controller. The light source emits light on the surface. The collector is configured to detect photoelectrons emitted from the surface in response to the light from the light source impinging on the surface. The collector is further configured to provide a photocurrent based on the detected photoelectrons. The controller includes at least one processor and is operably coupled to the light source and the collector. The controller is configured to cause the light source to emit light on the surface, receive the photocurrent from the collector, and determine at least one chemical reaction of the surface based on the received photocurrent.

Abstract: There is provided a pipette tip disposal assembly. The assembly comprises a receiving element adapted to support an end of at least one pipette tip, the at least one pipette tip being toppled in use from the receiving element along a first direction; and a container positioned to receive the at least one toppled pipette tip, the at least one pipette tip falling towards a base of the container on being received by the container. The base of the container has a first linear dimension aligned with the first direction corresponding to the length of a pipette tip.

Abstract: A reaction processor includes: a vessel installation unit for installing a reaction processing vessel provided with a channel formed in a substrate; a high temperature heater and a medium temperature heater for adjusting the temperature of the channel of the reaction processing vessel; a vessel alignment mechanism for adjusting the position of the reaction processing vessel 10; and a housing that has a housing main unit and a cover portion capable of being opened and closed with respect to the housing main unit and that houses the vessel installation unit, the high temperature heater, the medium temperature heater, and the vessel alignment mechanism. In conjunction with the state of the cover portion being changed from an open state to a closed state, the vessel alignment mechanism aligns the reaction processing vessel such that the reaction processing vessel can be heated by the high temperature heater and the medium temperature heater.

Abstract: A microfluidic device having an array for cell trapping is used to analyze cell-cell interaction at single-cell level. The microfluidic trapping array efficiently pairs single cells in isolated compartments in an easy-to-operate manner. A first cell is squeezed through an opening of a first cavity by a strong forward flow. Subsequently, a second cell is pushed into a second cavity by a low reverse flow. The trapped cell pairs are sealed by an oil phase or hydrogel into isolated compartments, thereby eliminating interference from other cell pairs or the surrounding media.

Abstract: Systems, devices, kits, and methods for detecting and quantifying targeted compounds within a liquid (such as urine) are provided. Such systems, devices, and methods may be autonomous, noninvasive, and provide quick and accurate results. The systems and devices are at least partially disposable (single-use) and configured to be embedded within or applied to a conventional diaper or the like. Methods for using the systems and devices hereof include receiving a liquid to be tested within a portion of a disposable device, allowing the liquid to traverse through one or more channels defined within the device in a controlled fashion, reacting the liquid with one or more chemical reagents, using a sensing unit to collect photocurrent data regarding the chemical reach on(s), and wirelessly transmitting that data to a computing unit for storage and quantitative analysis.

Abstract: A sorbent composition with improved acid gas reactivity comprising calcium hydroxide particles is provided. In the calcium hydroxide composition, the amount of time it takes the calcium hydroxide particles to neutralize in citric acid of a mass greater than 10 times the mass of the calcium hydroxide particles is less than 10 seconds; about 90% of the calcium hydroxide particles are less than or equal to about 10 microns; and the calcium hydroxide particles have a BET surface area of about 18 m2/g or greater.

Abstract: A material that can be used for detecting at least one alkaline element in cationic form chosen from polymers or inorganic materials, the material being functionalised by at least one group, referred to as group A, comprising one or more aromatic rings, the or all or a portion of the aromatic rings comprising at least one imine substituent and the or all or a portion of the aromatic rings comprising at least one atom carrying a free doublet within the or all or a portion of the rings and/or at least one other substituent different from an imine substituent comprising at least one atom carrying a free doublet.

Abstract: The current invention relates to the method and apparatus to separate biological entities from a fluid sample. The claimed methods separate biological entities based on size of entities by using acoustic pressure nodes in a microfluidic device. The claimed methods further separate biological entities with magnetic labels and by using a magnetic device. The claimed methods further include combination of microfluidic devices and magnetic devices to separate biological entities from a fluid sample.

Abstract: A blood glucose measurement reagent includes glucose dehydrogenase; a chromogenic indicator; and an aromatic hydrocarbon having at least one sulfonic acid group.