Patents Examined by Pensee T. Do
  • Patent number: 8071393
    Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: July 24, 2007
    Date of Patent: December 6, 2011
    Assignee: BioArray Solutions, Ltd.
    Inventor: Michael Seul
  • Patent number: 8039271
    Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: February 12, 2007
    Date of Patent: October 18, 2011
    Assignee: BioArray Solutions, Ltd.
    Inventor: Michael Seul
  • Patent number: 7943398
    Abstract: Embodiments of the invention relate generally to ferromagnetic microdisks, methods of detecting target bioanalyte using ferromagnetic microdisks, and kits (such as for using in the laboratory setting) containing the reagents necessary to make, and/or use ferromagnetic microdisks for bioanalyte detection, depending on the user's planned application. The methods and products allow the fabrication of ferromagnetic microdisks, and their use in the detection of biological molecules with high sensitivity, little or no signal decay, improved safety, convenience, and lowered cost for use and disposal.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: May 17, 2011
    Assignee: Intel Corporation
    Inventor: Chang-Min Park
  • Patent number: 7939283
    Abstract: Assays, such as immunoassays, and related articles are disclosed.
    Type: Grant
    Filed: July 12, 2002
    Date of Patent: May 10, 2011
    Assignee: Fisher Scientific Company L.L.C.
    Inventors: Teresa Chan, Joseph F. Lawlor, Gordon C. Siek, Arleen Chase, Joseph D. Musto
  • Patent number: 7935539
    Abstract: An improved agglutination immunoassay is characterized by reacting a sample fluid which may contain an analyte with a generic antibody conjugated to latex particles and then adding an antibody specific to the analyte to be determined. Agglutination resulting from adding the antibody specific to the analyte is measured and correlated with the amount of analyte in the sample.
    Type: Grant
    Filed: February 14, 2003
    Date of Patent: May 3, 2011
    Assignee: Siemens Healthcare Diagnostics, Inc.
    Inventors: David Brock, Jim Mattler, Gary Rheinheimer
  • Patent number: 7927827
    Abstract: A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.
    Type: Grant
    Filed: May 21, 2007
    Date of Patent: April 19, 2011
    Assignee: Kent State University
    Inventors: Gary D. Niehaus, Christopher J. Woolverton, Kathleen J. Doane, Steven P. Schmidt, Steven A. Signs
  • Patent number: 7919333
    Abstract: The invention relates to spherical and magnetic silicagel particles which are provided with an increased surface by incorporating SiO2 colloids and produced by a method for inverse dispersion cross-linking of silica sols. The addition of certain metallic oxides to said silica sols and a subsequent tempering make it possible to obtain silicagel particles which exhibit increased nucleic acid binding properties and are used for separating a nucleic acid and isolating biomolecules.
    Type: Grant
    Filed: November 23, 2004
    Date of Patent: April 5, 2011
    Assignee: MagnaMedics GmbH
    Inventor: Detlef Müller-Schulte
  • Patent number: 7910382
    Abstract: The present invention provides liquid crystal-based devices and methods for bioagent detection. In certain aspects, the present invention is directed to devices and methods utilizing liquid crystals and membranes containing polymerized targets that can report the presence of bioagents including, but not limited to, enzymes, antibodies, and toxins.
    Type: Grant
    Filed: October 31, 2006
    Date of Patent: March 22, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Nicholas L. Abbott, Joon-Seo Park, Sarah Teren, David J. Beebe, Eric A. Johnson
  • Patent number: 7906345
    Abstract: Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity.
    Type: Grant
    Filed: April 22, 2004
    Date of Patent: March 15, 2011
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Shan X Wang, Robert L White, Chris D Webb, Guanxiong Li
  • Patent number: 7897106
    Abstract: Contemplated herein is an automated microscope slide antigen recovery and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments for individually and independently processing a plurality of individual microscope slides. The apparatus preferably features independently movable slide support elements each having an individually heatable heating plate. Each slide support element preferably supports a single microscope slide. Each microscope slide can be enclosed within an individual pressurizable reaction compartment. Pressures exceeding 1 atm or below 1 atm can be created and maintained in the reaction compartment prior to, during or after heating of the slide begins.
    Type: Grant
    Filed: May 24, 2006
    Date of Patent: March 1, 2011
    Inventors: Lee Angros, Thomas Lee Byers
  • Patent number: 7892856
    Abstract: Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.
    Type: Grant
    Filed: December 26, 2006
    Date of Patent: February 22, 2011
    Assignee: Battelle Memorial Institute
    Inventors: Jay W. Grate, Cynthia J. Bruckner-Lea, David A. Holman
  • Patent number: 7892854
    Abstract: The present invention provides methods and apparatus for the application of a particle array in bioassay format to perform qualitative and/or quantitative molecular interaction analysis between two classes of molecules (an analyte and a binding agent). The methods and apparatus disclosed herein permit the determination of the presence or absence of association, the strength of association, and/or the rate of association and dissociation governing the binding interactions between the binding agents and the analyte molecules. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
    Type: Grant
    Filed: June 21, 2001
    Date of Patent: February 22, 2011
    Assignee: BioArray Solutions, Ltd.
    Inventors: Sukanta Banerjee, Michael Seul, Alice X. Li, Kairali Podual, Chiu W. Chau
  • Patent number: 7879625
    Abstract: Improved surface-enhanced Raman scattering (SERS) substrates comprising chemically-derivatized magnetic microparticles complexed with metal colloidal particles or substrates. The SERS substrates permit collection, detection, measurement, and/or analysis of analytes present at concentrations ranging parts per trillion to parts per billion. Further, compositions, methods, and devices that provide for rapid and/or sensitive detection of chemical compounds of interest present in small concentrations. The subject matter has use in the areas of homeland security and force protection, for example, in the detection of trace samples including, for example, BTEX (benzene, toluene, ethylbenzene, and xylenes), chlorinated solvents, TNT, nerve agents, blister agents, metal ions, anions, antigens, peptides, nucleic acids, spores, fungi, viruses, and bacteria.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: February 1, 2011
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventor: Pamela A. Boss
  • Patent number: 7875466
    Abstract: This invention provides nanometer-sized fluorescent magnetic particles and processes of making them. The nanoparticle has a core particle comprising a magnetic material and a fluorescent material, and the particle size is less than about 1 micrometer. The nanoparticles can be coated with an inorganic or organic layer and can be surface-modified. The nanoparticles can be used in many biological assays.
    Type: Grant
    Filed: August 13, 2003
    Date of Patent: January 25, 2011
    Assignees: CapitalBio Corporation, Tsinghua University
    Inventors: Huachang Lu, Guangshun Yi, Depu Chen, Lianghong Guo, Jing Cheng
  • Patent number: 7842516
    Abstract: Labels, methods of making labels and methods of using labels are disclosed. The labels can be manufactured using fiber drawing techniques or by shutter masking. The labels can be used for detecting the presence of an analyte in a sample and for detecting interactions of biomolecules.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: November 30, 2010
    Assignee: Corning Incorporated
    Inventors: Matthew J. Dejneka, Joydeep Lahiri, Uwe R. Muller, Cameron W. Tanner, Patrick D. Tepesch
  • Patent number: 7829348
    Abstract: The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).
    Type: Grant
    Filed: August 31, 2004
    Date of Patent: November 9, 2010
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Marc D. Porter, Jing Ni, Robert J. Lipert, G. Brent Dawson
  • Patent number: 7824926
    Abstract: The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).
    Type: Grant
    Filed: September 24, 2001
    Date of Patent: November 2, 2010
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Marc D. Porter, Jing Ni, Robert J. Lipert, G. Brent Dawson
  • Patent number: 7820402
    Abstract: The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase.
    Type: Grant
    Filed: September 19, 2005
    Date of Patent: October 26, 2010
    Assignee: Fuji Photo Film Co., Ltd.
    Inventors: Yoshiki Sakaino, Hitomi Ito, Toshihiro Mori, Osamu Seshimoto, Toshihisa Ito, Yoshikazu Amano
  • Patent number: 7785902
    Abstract: Disclosed is a method of analysis of a mixture of biological and/or chemical components that entails spatially arranging a chosen component attached to magnetic particles, exposing the particles to a magnetic field, and recording a magnetic induction signal, from which the content of the analyte in the mixture is judged; this includes grouping the chosen component in a probe volume, making the magnetic field alternating, pre-setting its spectrum, at least, at two frequencies, and recording the signal at a frequency, which is a linear combination of these frequencies, during the exposure of the magnetic particles to the field.
    Type: Grant
    Filed: March 7, 2001
    Date of Patent: August 31, 2010
    Assignee: Magnisense Technology Limited
    Inventors: Petr Ivanovich Nikitin, Petr Mikhailovich Vetoshko
  • Patent number: 7781182
    Abstract: The invention relates to assays for measuring ubiquitin ligase activity and for identifying modulators of ubiquitin ligase enzymes.
    Type: Grant
    Filed: January 9, 2006
    Date of Patent: August 24, 2010
    Assignee: Rigel Pharmaceuticals, Inc.
    Inventors: Sarkiz D. Issakani, Jianing Huang, Julie Sheung, Todd R. Pray