Abstract: An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
Abstract: A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t.
Abstract: A host vector system comprising Rhizopus species such as Rhizopus niveus (IFO4810) and a plasmid derived from Phycomycetes such as Mucor, Phycomyces and Absidia wherein the plasmid is contained in a chromosome and/or cytoplasm of the Rhizopus species. The host vector system is suitable for the extracellular production of proteins and enzymes. A process for preparing the host vector system is also provided.
Type:
Grant
Filed:
December 14, 1993
Date of Patent:
July 25, 1995
Assignee:
Nihon Shokuhin Kako Co., Ltd.
Inventors:
Masamichi Takagi, Hiroyuki Horiuchi, Koji Yanai, Kenji Sakaguchi
Abstract: Methods and recombinant vectors suitable for accomplishing the in vivo alteration of a nucleic acid molecule are disclosed. The invention in particular discloses the use of recombinases such as Cre to accomplish in vivo recombination.
Abstract: The present invention relates to the unexpected discovery that xanthan gum can be produced from filtered whey or milk products, including whey permeates and milk permeates, by a fermentation process which uses organisms which are capable of converting lactose to xanthan gum.
Type:
Grant
Filed:
May 11, 1993
Date of Patent:
July 18, 1995
Assignees:
Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
Inventors:
Thomas J. Pollock, Richard W. Armentrout
Abstract: A method for sequential cloning of chromosomal DNA of a target organism is disclosed. A first DNA segment homologous to the chromosomal DNA to be sequentially cloned is isolated. The first segment has a first restriction enzyme site on either side. A first vector product is formed by ligating the homologous segment into a suitably designed vector. The first vector product is circularly integrated into the target organism's chromosomal DNA. The resulting integrated chromosomal DNA segment includes the homologous DNA segment at either end of the integrated vector segment. The integrated chromosomal DNA is cleaved with a second restriction enzyme and ligated to form a vector-containing plasmid, which is replicated in a host organism. The replicated plasmid is then cleaved with the first restriction enzyme. Next, a DNA segment containing the vector and a segment of DNA homologous to a distal portion of the previously isolated DNA segment is isolated.
Type:
Grant
Filed:
December 20, 1991
Date of Patent:
July 18, 1995
Assignee:
The United States of America as represented by the United States Department of Energy
Abstract: This invention relates to DNA encoding drug resistance to cis-platin. The invention also includes expression products of, and vectors and hosts comprising the DNA sequence encoding cis-platin resistance. Also included are immunodiagnostic assays of cis-platin resistance and assays for screening materials having a modulating effect on DNA encoding the cis-platin resistance gene and on the expression product thereof. The invention is further directed to antagonists to the cis-platin resistance gene and the expression product thereof. Recombinant and pharmaceutical means making use of the cis-platin resistance gene and its expression product are also provided.
Type:
Grant
Filed:
March 8, 1993
Date of Patent:
July 18, 1995
Assignee:
The Regents of the University of California
Abstract: Disclosed are the plasmid pBUL1 having a restriction endonuclease cleavage map as shown in FIG. 1 and having a length of about 7.9 kbp and its derivatives.The plasmid was isolated from Lactobacillus delbrueckii subsp. bulgaricus M-878.The plasmid is useful as a vector for breeding various microorganisms such as lactic acid bacteria, and the derivatives thereof are useful also as a shuttle vector (lactic acid bacteria--Escherichia coli).
Abstract: A recombinant plasmid vector is provided which is capable of replicating and expressing in a Coryneform bacterial cell, which plasmid vector is pEC 701, pEC 702, pEC 801, pEC 830 or pEC 901.
Abstract: A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence and the vector being capable of being integrated into a chromosome of the host yeast cell; and a diploid or greater ploidy yeast cell transformed by such a vector with heterologous DNA.
Type:
Grant
Filed:
January 24, 1990
Date of Patent:
June 6, 1995
Assignee:
Robert R. Yocum
Inventors:
Robert R. Yocum, Robert S. Daves, Michael C. Chen
Abstract: The present invention contemplates a method of physiologic engineering by genetically altering second messenger levels in cells. This method allows the hyperactivation or inhibition of cell function within cells, tissues and animals by introducing a foreign gene that alters a second messenger system. The use of physiologically engineered animals as systems for determining the effectiveness of therapeutic compositions is also contemplated.
Abstract: The invention relates to an artificial promoter for the expression of proteins, especially urate oxidase in yeast, which comprises:a sub-sequence upstream from the TATA component of the sequence of the promoter of the GAL7 gene of Saccharomyces cerevisiae, which comprises the upstream activation sequences UAS1 and UAS2; anda sub-sequence of the sequence of an ADH.sub.2 promoter comprising the TATA component and the transcription initiation region.
Type:
Grant
Filed:
July 6, 1993
Date of Patent:
April 18, 1995
Assignee:
Sanofi
Inventors:
Pascal Leplatois, Gerard Loison, Bernard Pessegue, David Shire
Abstract: The isolation and characterization of the promoter regions of the genes which code for the pilinic subunits fim2, fim3 and fimx of Bordetella pertussis, are described, as well as the construction of vectors containing the regions, and microorganisms transformed by the vectors.The promoter regions, or nucleotide fragments thereof, are particularly useful for the regulable or non-regulable expression of genes which code for a protein of interest in a strain of Bordetella. The transformed Bordetella strains are particularly suitable for the development of an effective anti-pertussis vaccine.
Type:
Grant
Filed:
March 16, 1994
Date of Patent:
March 7, 1995
Assignee:
Eniricerhce S.p.A.
Inventors:
Barbara Riboli, Paola Pedroni, Anna Cuzzoni, Francesca de Ferra, Guido Grandi
Abstract: The invention relates to genes, vectors and Bacilli transformed with them which are useful in the hyperproduction of enzymes. The invention also relates to processes for producing enzymes using the genes, vectors and organisms of the invention.
Abstract: The present invention relates to DNA sequences useful in directing low to moderate expression of proteins in E. coli. The sequences are on based on modification of the repressor binding site of the tetA gene of transposon Tn10.
Abstract: Sample DNA is analyzed by joining dsDNA sample fragments to labeling moieties having a primer binding sequence, to provide labeled dsDNA. After denaturation of the labeled dsDNA, strands binding to a probe are separated, conveniently using particles and a specific binding pair, followed by amplification of the sample strands and analysis and/or isolation of the amplified strands.
Abstract: Isolated DNA or RNA molecules capable of hybridizing under stringent conditions to the myoD regulatory region, its proximal promoter and distal enhancer regulatory regions, and regulatory elements within the proximal and distal regions for binding basic helix-loop-helix proteins, MyoD, and proteins binding at SP1, AP1, CAAT, M-CAT, CArG, and MEF sites in DNA. DNA or RNA expression vectors for introducing a gene into a cell under the regulatory control of the myoD regulatory region. Transduced or transfected pre-muscle cells, myocytes, or myoblasts. Methods of inducing a muscle phenotype in a non-muscle cell, positively selecting for a cells expressing MyoD, and negatively selecting for cells expressing MyoD.
Type:
Grant
Filed:
September 3, 1991
Date of Patent:
October 4, 1994
Assignee:
Fred Hutchinson Cancer Research Center
Inventors:
Stephen J. Tapscott, Harold M. Weintraub, Theodore D. Palmer
Abstract: An E. coli heterologous gene expression system comprising a multicopy expression vector comprising an F promoter from bacteriophage P2; and at least one copy of a DNA sequence comprising a delta gene from satellite P4 operably linked to a regulatable promoter on a different replicon is disclosed.
Abstract: The kil-kor system from the plasmid pIJ101 can be utilized for regulated gene expression in Streptomycetes. For this, the KorA protein is either inactivated or eliminated to "switch on" a particular gene.
Type:
Grant
Filed:
July 23, 1993
Date of Patent:
August 9, 1994
Assignee:
Hoechst Aktiengesellschaft
Inventors:
Wolfgang Wohlleben, Gunter Muth, Alfred Puhler, Gunther J. Rieb
Abstract: An improved method for detecting antibodies is disclosed. The method involves the steps of a) mixing the specimen with a diluent comprising superoxide dismutase, and b) contacting the diluted specimen with at least one recombinant antigen expressed as a fusion protein with superoxide dismutase.
Type:
Grant
Filed:
May 10, 1993
Date of Patent:
July 19, 1994
Assignee:
Abbott Laboratories
Inventors:
Adrienne L. Gilbert, James L. Stewart, Sarah L. Kidd, George J. Dawson