Abstract: Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.
Type:
Grant
Filed:
July 8, 1999
Date of Patent:
June 14, 2005
Assignees:
Genetics Institute, Inc., Regents of the University of Michigan, The United States of America as represented by the Secretary of the Navy
Inventors:
Carl H. June, Craig B. Thompson, Gary J. Nabel, Gary S. Gray, Paul D. Rennert
Abstract: DNA encoding Antibodies to tumor necrosis factor receptors (TNF-Rs) are disclosed. The antibodies are preferably those which inhibit the cytotoxic effect of TNF but not its binding to the TNF-Rs. Most preferably, the antibodies bind to an extracellular domain of the C-terminal cysteine loop of the p75 TNF receptor, which loop consists of the amino acid sequence Cys-185 to Thr-201 of SEQ ID NO:3.
Type:
Grant
Filed:
March 8, 2001
Date of Patent:
August 5, 2003
Assignee:
Yeda Research and Development Co. Ltd.
Inventors:
David Wallach, Jacek Bigda, Igor Beletsky, Igor Mett, Hartmut Engelmann