Abstract: The present disclosure relates to selected antigenic proteins obtained from the outer membranes of Moraxella catarrhalis, that have been found by the inventors to have a variety of useful properties. These proteins, termed OMPs ("Outer Membrane Proteins"), are characterized as having molecular weights of 30, 80 and 100 kD, respectively. Studies set forth herein demonstrate that monoclonal antibodies directed against these proteins confer a protective effect against infection by Moraxella catarrhalis organisms in animal models, demonstrating the potential usefulness of such antibodies in conferring passive immunity as well as the potential usefulness of these OMPs, or variants thereof, in the preparation of vaccines. Also disclosed are DNA segments encoding these OMPs, methods for preparing the antigens, or variants, through the application of recombinant DNA techniques, as well as diagnostic methods and embodiments.

Abstract: Membrane for use in the detection of an analyte having a closely packed array of self-assembling amphiphilic molecules and a plurality of ionophores. First and second ligands are attached to an end of the ionophores adjacent the surface of the membrane. The membrane is such that the binding of the first ligand to its specific binding partner prevents the flow of ions across the membrane via the ionophores. In addition, the binding of the second ligand to its specific binding partner prevents the binding of the first ligand to its specific binding partner.

Abstract: The macrophage cell line, IT-1 (KCCM-10038) was derived from normal human bone marrow. IT-1 cells are characterized by excellent adherence onto glass slides used in immunofluorescence assays, and the cells provide a positive result in the antinuclear antibody (ANA) test. Moreover, ANA tests performed with IT-1 cells were found to be more reliable and more easily interpreted than ANA tests performed with the customary HEp-2 cell line. Accordingly, IT-1 cells are particularly suitable for the diagnosis of autoimmune disease using the ANA test.

Abstract: A sensitive chemiluminescence immunoassay method for field detection of the presence or the amount of low chlorinated biphenyl compounds in a solution is disclosed. The assay has a five minute analysis time and a working range of detection as low as about 1 part per billion chlorinated biphenyl. Kits for the detection of chlorinated biphenyls are disclosed.