Abstract: The present invention is directed generally to devices and methods for manipulating laboratory pipettes in a programmable manner. The present invention is directed to an apparatus and methods for allowing a user to instruct the device to perform a specific process; identifying the type, location and identity of the consumables to be used; manipulating a plurality of pipettes for performing the liquid handling; monitoring the process during and after its execution; generating a detailed report for the plurality of actions. Other aspects of this invention include optimization of the liquid dispensing performances of a pipette; monitoring and controlling individual actions by means of vision; virtualization of the protocol definition by means of a reality augmented software interface; integration of the system in a conventional laboratory environment workflow.

Abstract: A system to determine the transfection status of at least one cell in a population of cells includes a fluidic device comprising a microfluidic channel and a detection zone comprising a detection electrode module comprising two electrodes configured to detect electrical impedance between the electrodes transversely across the channel in the detection zone; corresponding to a cell passing the detection zone, a pump fluidically coupled to the fluidic device and configured to pump the population of cells in a carrier liquid along the microfluidic channel, and a processor operatively coupled to the detection electrode module and configured to detect a change in electrical impedance corresponding to a cell passing the detection zone, compare the change in electrical impedance with a reference change in electrical impedance corresponding to a cell of known transfection status, and calculate the transfection status of the cell based on the comparison.

Abstract: Devices and methods for the deposition of reagents onto cells or tissue samples are disclosed. Also disclosed are reagent compositions suitable for dispensing via a droplet-on-demand system.

Abstract: The present disclosure discloses a sample preliminary screening chip, a specimen detecting method and a screening device. A data processor may be configured to control a sample solution containing a specimen to be added into a preliminary screening inlet of the sample preliminary screening chip, and control the sample solution in the preliminary screening inlet to enter a channel, successively to flow through a first preliminary screening area and a second preliminary screening area, and to flow out from a preliminary screening outlet so as to store a liquid with the specimen in a first preliminary screening area. In this way, the liquid containing the specimen may be screened preliminarily.

Abstract: Disclosed is an apparatus for enriching and screening a target object such as a cell, bacteria or biomolecule from a sample. The apparatus includes a plurality of multi-channel mesh bodies arranged in parallel, and each multi-channel mesh body includes a mesh body formed by intersecting a plurality of threads, a plurality of screen pores formed by intersecting the plurality of threads, and a capturing layer formed on the mesh body. The capturing layer includes a capturing material specifically binding with the target object.

Abstract: The present disclosure relates to an engineered, additively manufactured, microfluidic cellular structure formed from a plurality of cells, wherein the cells are each formed from a plurality of interconnected elements. The cells have voids and each cell is open at upper ends thereof. The cells each communicate at a point below its upper end with a common channel. The cells are each configured to accept a fluid and operate to channel the fluid into the common channel and to hold the fluid received therein for later selective withdrawal from the structure.

Abstract: A method for determining an amount of teriflunomide in a sample by mass spectrometry includes adding an internal standard to the sample, subjecting the sample to solid phase extraction, purifying the sample by high turbulence liquid chromatography, ionizing the sample to produce one or more teriflunomide ions detectable by mass spectrometry, determining the amount of the one or more teriflunomide ions by mass spectrometry, and using the amount of the one or more ions determined to further determine the amount of teriflunomide in the sample.

Abstract: The present disclosure provides systems and methods for bioprocessing. The systems and methods can be implemented using a chip (solid support comprising a bioprocessing chamber). The bioprocessing chamber can be fluidically connected to a feeding input channel. The chip can permit a fluid to flow from the feeding input channel through the bioprocessing chamber for cell seeding, perfusion, and/or expansion. The bioprocessing chamber can be in fluidic communication with a collection output. The cells can be harvested from the bioprocessing chamber through the collection output.

Abstract: Disclosed is a method of artificial intelligence-based robotic pipetting for cell preparation. The method includes training an artificial intelligence/machine learning system (AI/ML system) to classify images of biological specimens. The method includes storing the classified images. The method includes receiving an image object from an imaging system that includes a microscopy system, a camera system configured to receive imaging from the microscopy system, and a lighting system configured to illuminate a biological material. The method includes processing the image object, using the AJ/ML system to determine a presence of a retrievable target cell. The method includes positioning a robotic pipettor at a target physical orientation relative to the retrievable target cell. The method includes confirming the robotic pipettor's location at the target physical orientation, using AI/ML system.

Abstract: An assay cartridge for use in a molecular diagnostics assay having an assay region; a first end comprising a first engagement member for engaging with a support housing; and a second end comprising a second engagement member for engaging with a support housing. The second engagement member has a clip, the clip being formed by a resilient element extending from the second end of the cartridge and a fastening element extending from said resilient element. The clip has a release element extending from the resilient element actuatable to release the clip from a caddy in use.

Abstract: A method of collecting resin beads includes first to fourth steps. The first step is a step of preparing a sample on a thin film provided on an upper surface of a substrate. The second step is a step of irradiating the thin film with a laser beam and a laser beam with the laser beam and the laser beam being distant from each other. The third step is a step of producing a microbubble at a position irradiated with the laser beam and producing a microbubble at a position irradiated with the laser beam, by heating the sample by irradiation with the laser beams. The fourth step is a step of collecting a plurality of resin beads in a region between the microbubble and the microbubble by producing convection of the sample in a direction perpendicular to a direction of alignment of the microbubble and the microbubble.

Abstract: The invention provides for a system for sample extraction that includes (a) a sample extraction device comprising a tubular structure, at least one channel, a reservoir, a fluid flow unit, and at least one sensor; (b) a container; (c) a motion platform operatively coupled to the tubular structure; (d) an actuator operatively coupled to the motion platform; and (e) one or more computer processors operatively coupled to the at least one sensor, the actuator, and the fluid flow unit.

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

Abstract: Systems and methods for automated sample preparation and processing of protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic agents.

Abstract: An inspection apparatus is an inspection apparatus for inspecting a sample in which a plurality of light-emitting elements including a first light-emitting element and a second light-emitting element arranged around the first light-emitting element is formed, the inspection apparatus including an excitation light source that generates excitation light to irradiate the sample, a camera that images fluorescence from the sample, and a determining unit that calculates a relative luminance of fluorescence from the first light-emitting element based on the fluorescence from the first light-emitting element and fluorescence from the second light-emitting element imaged by the camera, and compares a calculated value based on an absolute luminance and the relative luminance of the fluorescence from the first light-emitting element with a predetermined threshold value, thereby determining a quality of the first light-emitting element.

Abstract: An automated laboratory system includes a work area with a plurality of predefined workstations. Each of the plurality of predefined workstations occupies a footprint of a standardized microtiter plate. At least one tool holder is structured to move tools above the work area and at least one transport tool is structured to be moved by the tool holder in order to relocate laboratory items within the work area. At least one pipetting tool is structured to be moved by the tool holder in order to pick up and release pipette tips. A holder for pipette tip carriers includes at least one carrying apparatus defining a rectangular through-opening, and a support apparatus connected to the carrying apparatus. An electronic control apparatus is connected to the tool holder and structured to control movements of the transport tool and at least one pipetting tool by the tool holder.

Abstract: A sample liquid-sending apparatus includes a placement portion, a suction mechanism, and a vibrator. A sample container is placed in the placement portion, the sample container containing a suspension of a sample. The suction mechanism includes a nozzle configured to be inserted into the sample container placed in the placement portion, and suctions the sample through the nozzle. The vibrator vibrates the nozzle.

Abstract: 3D nanochannel interleaved devices for molecular manipulation are provided. In one aspect, a method of forming a device includes: forming a pattern on a substrate of alternating mandrels and spacers alongside the mandrels; selectively removing the mandrels from a front portion of the pattern forming gaps between the spacers; selectively removing the spacers from a back portion of the pattern forming gaps between the mandrels; filling i) the gaps between the spacers with a conductor to form first electrodes and ii) the gaps between the mandrels with the conductor to form second electrodes; and etching the mandrels and the spacers in a central portion of the pattern to form a channel (e.g., a nanochannel) between the first electrodes and the second electrodes, wherein the first electrodes and the second electrodes are offset from one another across the channel, i.e., interleaved. A device formed by the method is also provided.

Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having a well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.

Abstract: The present invention provides a pipette tip, which can be used in in-vitro diagnostics, in particular in the diagnostic testing of body fluids, such as in coagulation testing. The Pipette tip contains two constituents in a spatially separated manner. The present invention furthermore provides a method of performing such diagnostics, e.g. coagulation analysis, and to the use of the pipette tip in such diagnostic testing.