Patents Examined by Rachel Heather Freed
  • Patent number: 5871751
    Abstract: A vaccine and method for treating fish susceptible to infection by Renibacterium salmoninarum is described. The vaccine comprises killed microorganisms that lack intact cell-surface-associated protein p57. The vaccine can be used in combination with additional materials, such as, without limitation, adjuvants, plasticizers, pharmaceutical excipients, antigens other than the cells lacking intact cell-surface-associated protein p57, diluents, carriers, binders, lubricants, glidants, aesthetic compounds, such as flavoring and coloring agents, and combinations thereof. The vaccine may be enteric-coated for oral delivery. The enteric coating generally comprises a polymer coating that is impervious to dissolution and/or degradation in the stomach, but is dissolved upon passing to the higher pH environments of the intestine. A preferred embodiment of the vaccine is made using spherical sugar microspheres.
    Type: Grant
    Filed: October 12, 1994
    Date of Patent: February 16, 1999
    Assignee: The State of Oregon Acting By and Through the State Board of Higher Education on Behalf of Oregon State University
    Inventors: John Mark Christensen, Steve Kaattari, Jon D. Piganelli, Gregory Wiens, Jia Ai Zhang
  • Patent number: 5739000
    Abstract: This invention provides a method of multi-parameter data analysis by means of a hierarchical attractor algorithmic engine. The method employs analyzing the data by construction of a population hierarchy, wherein the populations are not mutually exclusive, thereby providing an important analytical tool. The use of the hierarchical attractor algorithmic engine presents the user with far greater flexibility in such analysis, as overlapping populations can be separately examined.
    Type: Grant
    Filed: April 13, 1994
    Date of Patent: April 14, 1998
    Assignee: Becton Dickinson and Company
    Inventors: Pierre Bierre, Daniel E. Thiel
  • Patent number: 5693517
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
    Type: Grant
    Filed: February 2, 1995
    Date of Patent: December 2, 1997
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David H. Gelfand, Thomas W. Myers, Christopher L. Sigua
  • Patent number: 5691157
    Abstract: The present invention is directed to a method for detecting a mammal's prior exposure to radiation or radiomimetic agents. Labeled antibodies are employed to determine the quantity of transferrin receptors on the red blood cells of the mammal. The quantity of transferrin receptors on the red blood cells of the mammal is correlated to the mammal's prior exposure.
    Type: Grant
    Filed: October 24, 1995
    Date of Patent: November 25, 1997
    Assignee: The Research Foundation of State University of New York
    Inventors: Joseph K. Gong, Chester A. Glomski
  • Patent number: 5691206
    Abstract: A device for carrying out solid phase microextraction is a fiber contained in a syringe. The fiber can be solid or hollow. The syringe has a barrel and a plunger slidable within the barrel, the plunger having a handle extending from one end of the barrel. A hollow needle extends from an end of the barrel opposite to the plunger. The fiber is contained in the needle. When the plunger is depressed, the fiber extends beyond a free end of the needle and when the plunger is in a withdrawn position, the fiber is located within the needle. The syringe protects the fiber from damage. When it is desired to analyze a sample in a bottle having a septum, the needle is inserted through the septum and the plunger is depressed so that the fiber will extend into the sample. After one or two minutes, the plunger is moved to the withdrawn position so that the fiber will return to the needle and the syringe is withdrawn from the sample bottle.
    Type: Grant
    Filed: September 19, 1994
    Date of Patent: November 25, 1997
    Inventor: Janusz B. Pawliszyn
  • Patent number: 5681709
    Abstract: A method for preparing treated naturally occurring or synthetic particles using a blood cell processor is provided. The method comprises adjusting the blood cell processor to allow a dispersion of such particles to come into contact with a treatment material in an amount and for a period of time sufficient to modify all or a portion of the surface of such particles. In a preferred embodiment, the method comprises washing collected red blood cells; separating a selected volume of said washed red blood cells; and treating one portion of said separated washed cells with a selected proteolytic enzyme, to prepare a red blood cell reagent.
    Type: Grant
    Filed: January 17, 1995
    Date of Patent: October 28, 1997
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Dennis Mochnal, Janice Jakway, Gilbert J. Quinton
  • Patent number: 5679526
    Abstract: This invention is directed to a ligand-receptor assay for determining the presence of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a colloidal gold particle, in a fluid sample suspected of containing said target ligand comprising the steps of:a. contacting said fluid sample with said ligand analogue conjugate and said ligand receptor to form a homogeneous reaction mixture, the relative amounts of said ligand analogue conjugate and said ligand receptor being selected such that in the absence of said target ligand and subsequent to substantially equilibrium binding in said reaction mixture, substantially all of said ligand analogue conjugate is bound to said ligand receptor such that no unbound ligand analogue conjugate is detected as a result of the assay method;b.
    Type: Grant
    Filed: August 1, 1994
    Date of Patent: October 21, 1997
    Assignee: Biosite Diagnostics Incorporated
    Inventors: Kenneth F. Buechler, Gunars E. Valkirs, Richard R. Anderson
  • Patent number: 5672481
    Abstract: A particle separator is provided for collection and manipulation of target particles, e.g., target cells, in a closed sterile field condition. In one embodiment, closed sterile field conditions are maintained from separation through concentration and/or cryo treatment steps and/or transfusion. Preservation of closed sterile field condition are accommodated by using the same integrally coupled rigid-walled vessel for collection and concentration and transfer via integrally coupled conduits to a vessel for cryopreservation and/or transfusion.In one embodiment, a plurality of valves are responsive to a data processor for controlling the path of fluid flow through the particle separator. A plurality of sensors are provided for providing sensor signals indicative of fluid flowing through the cell separator. A peristaltic pump is responsive to the microprocessor assembly for controlling the speed and direction of fluid flow through the system.
    Type: Grant
    Filed: April 23, 1993
    Date of Patent: September 30, 1997
    Assignee: Cellpro, Incorporated
    Inventors: Billy W. Minshall, Patrick M. Maloney, Fred Mill, Shelly Heimfeld, Stanley Corpuz, Penny Thompson, Eric Peterson
  • Patent number: 5658734
    Abstract: The present invention relates to a process for synthesizing on a single substrate a plurality of chemical compounds having diverse structures. The process involves the use of a bilayer photoresist to build up selected regions of the array in a step wise fashion.
    Type: Grant
    Filed: October 17, 1995
    Date of Patent: August 19, 1997
    Assignee: International Business Machines Corporation
    Inventors: Phillip Joe Brock, William Dinan Hinsberg, Jeffrey William Labadie, Glenn McGall, Gregory Michael Wallraff
  • Patent number: 5658745
    Abstract: This invention relates to a cell enumeration immunoassay which uses a calibrated standard, i.e., a substance which behaves like the sample under study and the concentration of which can be correlated to the concentration of the cells. This immunoassay is an efficient alternative to flow cytometry.
    Type: Grant
    Filed: February 17, 1995
    Date of Patent: August 19, 1997
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: Richard Alfred Greene, Patricia Ann Kasila, Mark Norman Bobrow
  • Patent number: 5648225
    Abstract: A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
    Type: Grant
    Filed: August 25, 1994
    Date of Patent: July 15, 1997
    Assignee: Abbott Laboratories
    Inventors: Young Ran Kim, Johanna Kantor, James E. Gill, Sue E. Luptovic
  • Patent number: 5643796
    Abstract: A droplet flow cytometer system which includes a system to optimize the droplet formation time delay based on conditions actually experienced includes an automatic droplet sampler which rapidly moves a plurality of containers stepwise through the droplet stream while simultaneously adjusting the droplet time delay. Through the system sampling of an actual substance to be processed can be used to minimize the effect of the substances variations or the determination of which time delay is optimal. Analysis such as cell counting and the like may be conducted manually or automatically and input to a time delay adjustment which may then act with analysis equipment to revise the time delay estimate actually applied during processing. The automatic sampler can be controlled through a microprocessor and appropriate programming to bracket an initial droplet formation time delay estimate.
    Type: Grant
    Filed: October 14, 1994
    Date of Patent: July 1, 1997
    Assignee: University of Washington
    Inventors: Ger Van den Engh, Richard J. Esposito
  • Patent number: 5643535
    Abstract: A luminometer is described comprising a tray for receiving an array of sample wells, a photodetector assembly, and means for relatively moving the tray and photodetector assembly in one direction to align the sample wells received by the tray in a predetermined sequence with the photodetector assembly. The photodetector assembly of the luminometer includes a stage, a photodetection head having a detection aperture permitting passage of light therethrough, a means for mounting the photodetection head to the stage that permits movement of the head in a direction substantially normal to the direction of relative tray and photodetector assembly movement, and a means for biasing the photodetection head toward a selected sample well so that the detection aperture is substantially isolated from light emitted from adjacent wells. Internal and external photodetector calibration systems and a sample heater are also contemplated.
    Type: Grant
    Filed: May 11, 1995
    Date of Patent: July 1, 1997
    Assignee: Chiron Corporation
    Inventors: Rick T. Smethers, Brian D. Warner, Victor P. Burolla
  • Patent number: 5643799
    Abstract: The process of this invention comprises providing a membrane for separating CO.sub.2 into a first CO.sub.2 sample phase and a second CO.sub.2 analyte phase. CO.sub.2 is then transported through the membrane thereby separating the CO.sub.2 with the membrane into a first CO.sub.2 sample phase and a second CO.sub.2 analyte liquid phase including an ionized, conductive, dissociated CO.sub.2 species. Next, the concentration of the ionized, conductive, dissociated CO.sub.2 species in the second CO.sub.2 analyte liquid phase is chemically amplified using a water-soluble chemical reagent which reversibly reacts with undissociated CO.sub.2 to produce conductivity changes therein corresponding to fluctuations in the partial pressure of CO.sub.2 in the first CO.sub.2 sample phase. Finally, the chemically amplified, ionized, conductive, dissociated CO.sub.2 species is introduced to a conductivity measuring instrument. Conductivity changes in the chemically amplified, ionized, conductive, dissociated CO.sub.
    Type: Grant
    Filed: October 31, 1994
    Date of Patent: July 1, 1997
    Assignee: Umpqua Research Company
    Inventors: James E. Atwater, James R. Akse, Jeffrey DeHart
  • Patent number: 5641637
    Abstract: A method is disclosed for preparing lyophilized and frozen cells as cytometry standards.
    Type: Grant
    Filed: April 5, 1993
    Date of Patent: June 24, 1997
    Assignee: Cobe Laboratories, Inc.
    Inventors: Robert Hudak, Iris Williams, Vijay Adda, Raymond P. Goodrich, Jr.
  • Patent number: 5637458
    Abstract: A system for biomolecular separation and detection of a molecular species includes a solid state laser detector having a sample channel therein. The presence of a molecular species is indicated by a frequency shift in the laser's output, which is detected by optical heterodyning the laser's output with the output of a reference laser. The interior of the sample channel is optionally coated with a ligand for binding the molecular species of interest. The system involves preprocessing a sample by electroosmotic separation in channels that are lithographically formed in a two-dimensional planar substrate. Molecular separation is also accomplished in a nanostructural molecular sieve comprising spaced apart posts defining narrow channels therebetween.
    Type: Grant
    Filed: July 20, 1994
    Date of Patent: June 10, 1997
    Assignee: Sios, Inc.
    Inventors: Robert Frankel, James M. Forsyth
  • Patent number: 5635136
    Abstract: A combustible gas sensor having a noble metal sensor element with a surface that is heated above a critical temperature at which the surface is able to dissociate oxygen in a gas stream and adsorb the oxygen onto its surface. The adsorbed oxygen present on the noble metal surface enhances the reactivity of the sensor element and permits it to react with combustible gases that otherwise would have little or no affinity for the sensor element. The balance of adsorbed oxygen and combustible gas species on the sensor surface cause a change in an electrical property that is used to determine the presence or identity of a combustible gas, or to derive a concentration measurement.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: June 3, 1997
    Assignee: Arizona Board of Regents
    Inventors: William Glaunsinger, Ian Sorensen, Mahesan Chelvayohan
  • Patent number: 5633172
    Abstract: A method is provided for analyzing an impurity on a semiconductor substrate. In this method, those to-be-measured areas on a surface of a semiconductor substrate are exposed with an ultraviolet radiation to provide corresponding oxide films. By doing so, an impurity is trapped in the oxide film at each area of the semiconductor substrate. The surface of the semiconductor substrate is cleaned with an acid solution to remove an impurity on other than the areas on the surface of the semiconductor substrate. The surface of the semiconductor substrate is exposed with a hydrofluoric acid vapor to dissolve the oxide films into very fine droplets containing an impurity. These droplets are moved on the surface of the semiconductor substrate to collect these droplets into a drop on the surface of the semiconductor substrate. The impurity in the drop is measured by a known chemical spectrometer.
    Type: Grant
    Filed: December 7, 1994
    Date of Patent: May 27, 1997
    Assignee: Kabushiki Kaisha Toshiba
    Inventor: Ayako Shimazaki
  • Patent number: 5633166
    Abstract: In high volume laboratory analysis of patient medical specimens, control materials are inserted at periodical intervals (e.g. once per eight hours) and analyzed. If the results of the analysis deviate from the mean according to statistical rules, the run of analyses prior to the insertion of control materials has in the past been rejected, often causing a substantial loss of results because the control materials were not inserted sufficiently frequently. According to the invention the patient mean of a test is determined, and when the mean deviates from the predetermined mean by more than a selected amount, this determination is used, not to reject the run, but rather to trigger insertion and analysis of control materials. If the analysis of control materials shows that the run is in control, the analyses made prior thereto are reported and the run is resumed. If the control material analysis shows that the run is out of control, then the prior analyses are rejected.
    Type: Grant
    Filed: January 19, 1995
    Date of Patent: May 27, 1997
    Assignee: MDS Health Group Limited
    Inventors: James O. Westgard, Paul Mountain
  • Patent number: 5633167
    Abstract: Methods for blood sample preparation using reagent compositions which include a zwitterionic surfactant for sphering of cells to eliminate orientational noise. When the reagent composition including a dye for staining a subset of cells is reacted with a blood sample, and the reaction mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the stained cells can be distinguished from the unstained cells, and when the cells are reticulocytes and mature erythrocytes, respectively, the volume and hemoglobin concentration of each reticulocyte or erythrocyte, and the hemoglobin content, mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.
    Type: Grant
    Filed: December 22, 1994
    Date of Patent: May 27, 1997
    Assignees: Bayer Corporation, Mt. Sinai School of Medicine of the City Univ. of New York
    Inventors: Sophie S. Fan, Daniel Ben-David, Albert Cupo, Gena Fischer, Grace E. Martin, Leonard Ornstein, Gregory M. Colella