Patents Examined by Ralph Gitomer
  • Enzyme compositions
    Patent number: 9322012
    Abstract: The present technology provides for enzyme compositions with enhanced enzyme activity, thermophilic and psychrophilic stability.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: April 26, 2016
    Assignee: UNIVERSITY OF CALCUTTA
    Inventors: Anjan Kr. Dasgupta, Tamoghna Bhattacharyya, Arka Mukhopadhyay, Nalok Dutta, Krishanu Chakraborty
  • Human monocyte sub-population for treatment of central nervous system injury
    Patent number: 9314483
    Abstract: A subpopulation of peripheral blood mononuclear cells (PBMCs) that is substantially devoid of CD3+ cells, CD19+ cells, CD56+ cells and optionally of CD16+ cells, for use in treatment of CNS injury is provided.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: April 19, 2016
    Assignee: Yeda Research and Development Co., Ltd.
    Inventors: Michal Eisenbach-Schwartz, Ester Yoles, Ravid Shechter, Omer Miller
  • Bacterium for use as a probiotic for nutritional and medical applications
    Patent number: 9314489
    Abstract: A first aspect of the invention relates to the bacterial species Roseburia hominis for use in: regulating the immune system of a subject treating an immune disorder; treating an intestinal disorder; improving intestinal microbiota; regulating the innate immune system of a subject; regulating the adaptive immune system of a subject; regulating appetite in a subject; promoting Tregs and immune tolerance; promoting gut health in a subject; and/or maintaining immune homeostasis in a subject. Further aspects of the invention relate to compositions comprising Roseburia hominis.
    Type: Grant
    Filed: October 8, 2012
    Date of Patent: April 19, 2016
    Assignee: 4D Pharma Research Limited
    Inventors: Denise Kelly, Imke Mulder
  • Process for the continuous enzymatic hydrolysis of proteins
    Patent number: 9315845
    Abstract: A continuous process and device for the enzymatic hydrolysis of proteins is disclosed.
    Type: Grant
    Filed: December 9, 2014
    Date of Patent: April 19, 2016
    Assignee: DMK Deutsches Milchkontor GmbH
    Inventor: Sven-Rainer Döring
  • Methods for inducing immune tolerance to organ transplants
    Patent number: 9295756
    Abstract: The present invention is directed to methods of treating an organ or a tissue prior to transplantation thereof into a recipient, comprising ablating the cells composing the blood vessels of said organ or tissue, preferably the cells lining the lumen surface of the blood vessels, thereby obtaining a treated viable organ or tissue having reduced immunogenicity. The invention is also directed to treated viable organs or tissues having reduced immunogenicity and uses thereof.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: March 29, 2016
    Assignee: NAYACURE THERAPEUTICS LTD.
    Inventor: Shahar Cohen
  • Fluorescent method of detecting microorganisms resistant to a therapeutic agent
    Patent number: 9290790
    Abstract: This invention relates to an antimicrobial susceptibility test made upon pure microbial cultures—a laboratory culture containing a single species of organism—or directly from biological samples uncultured. Some preferred embodiments do not require the use a pure culture obtained from a biological product which would otherwise take at least 24 hours for the microorganisms to grow—so, is performed an antimicrobial susceptibility test directly from uncultured biological samples like urine, blood cultures or water by fluorescence analysis.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: March 22, 2016
    Assignee: UNIVERSIDADE DO PORTO
    Inventors: Cidália Irene Azevedo Pina Vaz, Acácio Agostinho Goncalves Rodrigues, Isabel Cristina Santos Silva De Faria Ramos Antunes, Ana Sofia Quinta E Costa De Oliveira Morais, Ana Teresa Pinto E Silva, Ana Isabel Silva Dias, Rita Daniela Teixeira Dos Santos
  • Filter support with a phase-changing medium
    Patent number: 9291533
    Abstract: The present invention relates to a method for processing isolated cells, in particular rare cells such as circulating tumor cells, to render them suitable for optical imaging, comprising the steps of (a) depositing filter material comprising captured cells on a phase-change medium; (b) melting said phase-change medium until the medium is spread out below said filter material; and (c) lowering the temperature of said phase-change medium until the medium solidifies, resulting in an optically flat filter comprising said cells at a fixed position. The method may additionally comprise an initial step of capturing a cell on a filter material. The phase-change medium, preferably paraffin wax, may comprise a porous, or mesh-like structure allowing the passage of a fluid through the medium. The phase-change medium may further be mounted on a carrier such as a glass carrier or a polymer material carrier.
    Type: Grant
    Filed: July 4, 2012
    Date of Patent: March 22, 2016
    Assignee: Koninklijke Philips N.V.
    Inventors: Menno Willem Jose Prins, Mikhail Mikhaylovich Ovsyanko, Freek Van Hemert, Arie Rombertus Van Doorn
  • Method of analyzing the charge profile of heparan N-sulfatase
    Patent number: 9290793
    Abstract: The present invention provides, among other things, methods for the characterization of recombinant Heparan N-Sulfatase (HNS) during manufacture. The present invention uses capillary zone electrophoresis to determine the charge profile, isoform distribution, and/or glycan profile of recombinant HNS; and represents a quality feature for the batch consistency, storage stability, biological half-life, pharmacokinetic, pharmacodynamic and biological activity of the enzyme. In particular, such characterization methods may be beneficial to optimize conditions and ensure consistency for the manufacture of HNS for the treatment of a patient diagnosed with Sanfilippo syndrome using enzyme replacement therapy.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: March 22, 2016
    Assignee: Shire Human Genetic Therapies, Inc.
    Inventor: Daniel Roseman
  • Method and system for collecting leukoreduced red blood cells
    Patent number: 9284529
    Abstract: A method and system for collecting leukoreduced red blood cells employing a spinning membrane separator including a housing having an upper end region and a lower end region in an operating position with a red blood cell outlet in the upper end region of the housing and a whole blood inlet in the lower end region of the housing. The method and system provide for flowing additive solution into the whole blood inlet of the housing to prime the separator; flowing whole blood into the whole blood inlet of the housing; separating red blood cells from the whole blood; flowing separated red blood cells out of the red blood cell outlet of the housing; combining the separated red blood cells with additive solution; passing the separated red blood cells and additive solution combination through a leukoreduction filter; and collecting the filtered red blood cells and additive solution.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: March 15, 2016
    Assignee: Fenwal, Inc.
    Inventors: Benjamin Kusters, Christopher Wegener, Kyungyoon Min
  • Methods to assay kinase activity
    Patent number: 9284597
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: March 15, 2016
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Methods to assay phosphatase activity
    Patent number: 9284598
    Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: March 15, 2016
    Assignee: Fluidigm Canada Inc.
    Inventor: Olga Ornatsky
  • Method for flushing a leuikoreduction filter
    Patent number: 9279104
    Abstract: A blood processing system and method are disclosed that comprise a leukoreduction filter for removing leukocytes from red blood cells as the red blood cells pass through the filter; and a pump for pumping an additive solution through the leukoreduction filter to flush the leukoreduction filter of red blood cells that remain in the leukoreduction filter after filtration of the red blood cells. Additive solution is flowed through the leukoreduction filter at an initial flush rate to flush remaining red blood cells from the leukoreduction filter and the pump increases the flush rate of the additive solution during flushing of the leukoreduction filter.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: March 8, 2016
    Assignee: Fenwal, Inc.
    Inventors: Benjamin Kusters, Christopher Wegener, Kyungyoon Min
  • Method of detecting a biological activity
    Patent number: 9279141
    Abstract: The present invention provides method of detecting a predetermined biological activity. The method includes using an aqueous mixture comprising a first indicator reagent with a first absorption spectrum and a second indicator reagent. The second indicator reagent is converted by the predetermined biological activity to a second biological derivative with a second emission spectrum. The first absorbance spectrum includes detectable absorbance in at least a portion of wavelengths present in the second emission spectrum. The first indicator reagent is received and concentrated from an aqueous liquid by a substrate, facilitating the detection of the second biological derivative.
    Type: Grant
    Filed: August 7, 2014
    Date of Patent: March 8, 2016
    Assignee: 3M Innovative Properties Company
    Inventors: Sailaja Chandrapati, Heather M. Webb, Jeffrey C. Pederson, Kurt J. Halverson, Brian J. Engel
  • Media comprising a glutamine biosensor and methods of use thereof
    Patent number: 9279139
    Abstract: An essentially glutamine-free medium comprising a whole-cell glutamine biosensor, comprising a glutamine auxotrophic E. coli and a Lux reporter gene, for detecting glutamine in an analyte. The medium is useful in methods to detecting, screening, identifying and selecting nitrogen-fixing microbes and in methods of determining the nitrogen state of plants, plant products and soil.
    Type: Grant
    Filed: June 20, 2012
    Date of Patent: March 8, 2016
    Assignee: University of Guelph
    Inventors: Manish Raizada, Michael Tessaro, Hanan Reda Hassan Elsayed Shehata
  • Cell sorter and cell sorting method
    Patent number: 9279802
    Abstract: A cell sorter including a well that has a microspace filled with a liquid and having a typical length of 1 mm or less, and that has a bottom surface made of a light-permeable material allowing optical observation of an interior of the microspace; a matrix provided on the bottom surface in the well; a bone fragment placed on the matrix in the well; and osteoclasts placed between the matrix and the bone fragment.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: March 8, 2016
    Assignees: SHIMADZU CORPORATION, KYOTO UNIVERSITY
    Inventors: Tatsuya Munaka, Hirohisa Abe, Masaki Kanai, Taira Maekawa, Shinya Kimura, Eishi Ashihara
  • Assay utilising cellular binary half-adder system
    Patent number: 9274096
    Abstract: An in vitro cell assay for detecting an effect a test condition has on one or more cells. The assay comprises providing a binary half-adder system comprising first and second oscillating cells, and detecting an effect a test condition has on the oscillation of said first and/or second cells.
    Type: Grant
    Filed: March 31, 2014
    Date of Patent: March 1, 2016
    Assignee: Manchester Metropolitan University
    Inventors: Stephen Lynch, Jon Borresen, Mark Slevin
  • Methods for assessing cell culture fluid by impedance spectra
    Patent number: 9274071
    Abstract: Provided herein are techniques for identification of viable and nonviable cells in a cell culture that include measuring a resonance impedance spectral response of at least one resonator in proximity to the cell culture and correlating the measured response to the concentration of viable cells in cell culture and/or the concentration of nonviable cells in cell culture.
    Type: Grant
    Filed: December 30, 2013
    Date of Patent: March 1, 2016
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Radislav A. Potyrailo, Victoria E. Cotero, Jon A. Dieringer, Thomas J. Erdenberger, Timothy A. Wortley
  • Method for detecting a target microorganism using two indicator systems in a culture device
    Patent number: 9273340
    Abstract: A method of detecting a target microorganism is disclosed. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of an indicator microorganism. When an indicator microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect the target microorganism.
    Type: Grant
    Filed: December 23, 2011
    Date of Patent: March 1, 2016
    Assignee: 3M Innovative Properties Company
    Inventors: Takatoshi Moriyama, Patrick A. Mach, Akio Kitahara, Henry J. Lubrant
  • Aminoclay for harvesting algae
    Patent number: 9267105
    Abstract: The present invention relates to aminoclay, for harvesting algae, which is prepared by fixing aminosilane around cationic metal ions through a sol-gel reaction. Also, the present invention relates to a method for harvesting algae using the aminoclay according to any one selected from the above methods.
    Type: Grant
    Filed: March 13, 2012
    Date of Patent: February 23, 2016
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Wasif Farooq, Young-Chul Lee, Ji-Won Yang
  • Assay method using encoded particle-based platform
    Patent number: 9267950
    Abstract: Provided is an assay method using an encoded particle-based platform. In the assay method, first, a plurality of encoded particles having codes distinguishable from one another according to kinds of included target materials are prepared. The plurality of encoded particles are provided onto a plate including a plurality of wells by pipetting, and disposed in the plurality of wells by a self-assembly method. An analyte is provided into the plurality of wells. The codes of the plurality of encoded particles disposed in the plurality of wells are decoded. The target materials of the plurality of encoded particles are released to cause a reaction between the target materials and the analyte.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: February 23, 2016
    Assignee: SNU R&DB FOUNDATION
    Inventors: Sung Hoon Kwon, Su Eun Chung, Sung Hoon Lee, Wook Park, Young Hoon Song