Patents Examined by Rebecca M Fritchman
  • Patent number: 12131893
    Abstract: A method of identifying tobacco carbonyl components using non-targeted mass spectrometry is disclosed, which includes the following steps: (1) preparing a tobacco sample extract; (2) derivatizing (part of) the sample/extract with 2,4-dinitrophenylhydrazine (DNPH); (3) derivatizing (another part of) the sample/extract with DNPH-d3; (4) mixing the derivatized tobacco samples; (5) preparing blank samples; (6) performing ultra performance liquid chromatography (UPLC) and ion trap (Orbitrap) high resolution mass spectrometry (HRMS) on the (derivatized) tobacco samples; (7) processing the LC-MS data; (8) filtering the mass spectrometric characteristic data to retain final chromatographic peaks; and (9) structurally annotating or identifying the retained final chromatographic peaks to obtain the tobacco carbonyl components.
    Type: Grant
    Filed: October 30, 2023
    Date of Patent: October 29, 2024
    Assignee: CHINA TOBACCO YUNNAN INDUSTRIAL CO., LTD.
    Inventors: Ruizhi Zhu, Xiaoxi Si, Yanqun Xu, Zhihua Liu, Ji Yang, Wei Jiang, Chunbo Liu, Fengmei Zhang, Zhenjie Li, Shiyun Tang
  • Patent number: 12050227
    Abstract: The disclosure generally relates generally to lipid nanodiscs, in particular to lipid nanodiscs formed from acryloyl-based copolymers. A lipid nanodisc according to the disclosure includes a lipid bilayer having a first hydrophilic face and a second hydrophilic face opposing the first hydrophilic face, and a hydrophobic edge between the opposing hydrophilic faces, and an acryloyl-based copolymer encircling the hydrophobic edge of the lipid bilayer. The acryloyl-based copolymer includes a first monomer unit having a pendant hydrophobic group and a second monomer unit having a pendant hydrophilic group. Methods of making and characterizing the lipid nanodiscs are also disclosed.
    Type: Grant
    Filed: April 17, 2019
    Date of Patent: July 30, 2024
    Assignees: Nara Institute of Science and Technology, THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Ayyalusamy Ramamoorthy, Thirupathi Ravula, Bikash Sahoo, Kazuma Yasuhara, Jin Arakida, Jun-Ichi Kikuchi
  • Patent number: 12011714
    Abstract: The present disclosure is notably directed to a microfluidic probe head, or MFP head, comprising a processing surface having a liquid injection aperture and a liquid aspiration aperture thereon. The aspiration aperture is generally shaped so as to partly extend around the injection aperture on the processing surface, although such injection apertures are not completely surrounded by the slit on the processing surface. Further, fluidic and solid barriers to aspiration are considered. The disclosure is further directed to related microfluidic probe devices, and methods of operation of such an MFP head, notably to deposit cells on a surface.
    Type: Grant
    Filed: August 4, 2020
    Date of Patent: June 18, 2024
    Assignees: Bio-Rad Laboratories, Inc., Bio-Rad Europe Gmbh
    Inventors: Emilie Frisan, Laurent Guillon, Robert Lovchik, David P. Taylor, Claudia I. Trainito, Govind V. Kaigala
  • Patent number: 12013405
    Abstract: A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein and an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition as well as the reagent mixture obtained in this manner. Also disclosed is the use of the reagent compositions to release vitamin D compounds as well as a kit for detecting a vitamin D compound.
    Type: Grant
    Filed: October 26, 2021
    Date of Patent: June 18, 2024
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Sascha Antoni, Christian Vogl
  • Patent number: 11898961
    Abstract: A system, device and method for measuring markers for a periprosthetic joint infection in a sample of synovial fluid. A sensor reader device includes a vial receptacle for receiving a vial containing synovial fluid. A light source illuminates the vial and an optical sensor detects light scattered and/or absorbed by white blood cells in the fluid sample. An electrical signal corresponding to the intensity of the light received at the optical sensor is detected. A white blood cell concentration is determined from the electrical signal value. The sensor reader device also includes one or more immunoassay strip receptacles. Immunoassay strips for other marker are inserted into the receptacles. Immunoassay optical sensors detect light generated by the immunoassay reaction on the immunoassay strip to determine the concentration of the marker. In example implementations, the device includes an immunoassay strip reader for leukocyte esterase and c-reactive protein.
    Type: Grant
    Filed: April 2, 2020
    Date of Patent: February 13, 2024
    Assignee: Alio, Inc.
    Inventors: Forrest Miller, Samit Kumar Gupta, David John Kuraguntla, Elsa Swanson
  • Patent number: 11867649
    Abstract: A method for detecting cancerous status of a biological sample. The method includes calculating a charge transfer resistance (Rct) of an electrochemical impedance spectroscopy (EIS) associated with lipid secretion of a biological sample, detecting a cancerous state for the biological sample responsive to the calculated Rct being equal to or more than a threshold value, and detecting a normal state for the biological sample responsive to the calculated Rct being less than the threshold value. The biological sample includes a biological sample suspected to be cancerous.
    Type: Grant
    Filed: October 16, 2019
    Date of Patent: January 9, 2024
    Assignee: NANO HESGARSAZAN SALAMAT ARYA INCUBATION CENTER FOR MEDICALEQUIPMENT AND DEVICES
    Inventors: Mohammad Abdolahad, Ashkan Zandi
  • Patent number: 11834703
    Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
    Type: Grant
    Filed: September 13, 2021
    Date of Patent: December 5, 2023
    Assignee: Agilent Technologies, Inc.
    Inventors: Charles M. Hansen, Steen Hauge Matthiesen, Kenneth H. Petersen, Tim Svenstrup Poulsen
  • Patent number: 11808773
    Abstract: The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.
    Type: Grant
    Filed: March 22, 2021
    Date of Patent: November 7, 2023
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Brett Holmquist, Nigel Clarke
  • Patent number: 11798794
    Abstract: Provided are methods of detecting the presence or amount of the active form of vitamin B6, pyridoxal 5?-phosphate, in a body fluid sample using tandem mass spectrometry coupled with liquid chromatography.
    Type: Grant
    Filed: August 18, 2020
    Date of Patent: October 24, 2023
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Qibo Jiang, Sum Chan, Richard E. Reitz
  • Patent number: 11761968
    Abstract: The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.
    Type: Grant
    Filed: March 22, 2021
    Date of Patent: September 19, 2023
    Assignee: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
    Inventors: Brett Holmquist, Nigel Clarke
  • Patent number: 11674969
    Abstract: Provided herein are methods for high throughput quantitation of testosterone utilizing at least two different derivatizing agents of different masses. In another aspect, provided herein are methods for determining the amount of testosterone in each of a plurality of human samples with a single mass spectrometric assay by subjecting each of a plurality of human samples to a different derivatizing agent to generate a differently derivatized testosterone in each of the plurality of samples; combining the plurality of samples to form a multiplex sample; and quantifying the amount of testosterone in each sample by mass spectrometry.
    Type: Grant
    Filed: March 15, 2019
    Date of Patent: June 13, 2023
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Mildred M. Goldman, Scott Goldman, Julia Colletti, Nigel Clarke
  • Patent number: 11644464
    Abstract: Embodiments may include an automated method for evaluating a sepsis status associated with a blood sample obtained from an individual. Methods may include determining a standard deviation of monocyte volume associated with the blood sample. Methods may include determining a white blood cell count (WBC) associated with the blood sample. Methods may include evaluating, using a data processing module, the sepsis status associated with the blood sample. The data processing module may include a processor and a computer application. This computer application may cause the processor to compare the standard deviation of monocyte volume with a first cutoff value to provide a comparison. The computer application may cause the processor to compare the WBC to a second cutoff to provide a second comparison. The computer application may also cause the processor to evaluate the sepsis status associated with the blood sample based on the first comparison and the second comparison.
    Type: Grant
    Filed: April 22, 2019
    Date of Patent: May 9, 2023
    Assignee: Beckman Coulter, Inc.
    Inventors: Robert T. Magari, Diana B. Careaga, Liliana M. Tejidor
  • Patent number: 11622750
    Abstract: The disclosed apparatus, systems and methods relate to devices, systems and methods for the collection of bodily fluids. The collector can make use of microfluidic networks connected to collection sites on the skin of a subject to gather and shuttle blood into a removable cartridge. The collected fluid is supplied to substrate for drying, storage and transport.
    Type: Grant
    Filed: February 26, 2021
    Date of Patent: April 11, 2023
    Assignee: Tasso, Inc.
    Inventors: Ben Moga, Ben Casavant, Erwin Berthier
  • Patent number: 11604123
    Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: March 14, 2023
    Assignee: Waters Technologies Corporation
    Inventors: Bonnie A. Alden, Kenneth Berthelette, Jon Finch, Donna Osterman, Thomas Swann, Thomas H. Walter
  • Patent number: 11604199
    Abstract: In a method for immunologically quantifying a steroid, it is possible to exclude a steroid from cyclodextrin when a standard solution containing the steroid included in cyclodextrin is treated with a surfactant, which thereby suppresses the deviation in behavior of the antigen-antibody reaction between the specimen (test sample) and the standard solution, and then significantly improves the accuracy of steroid quantification.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: March 14, 2023
    Assignee: FUJIREBIO INC.
    Inventors: Tatsunari Yoshimi, Kyoko Teruyama
  • Patent number: 11597646
    Abstract: A device includes: a die including a microfluidic device; a polymer substrate formed around the die; and a separate fluid manifold attached to the polymer substrate over the die and on a same side of the substrate as the die, the manifold to deliver fluid to the die.
    Type: Grant
    Filed: July 26, 2016
    Date of Patent: March 7, 2023
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Chien-Hua Chen, Devin Alexander Mourey, Michael W. Cumbie, Si-lam Choy
  • Patent number: 11596943
    Abstract: Some embodiments of a micro-fluidic device include at least one inlet hole located on an inlet side of the microfluidic device, the inlet hole consisting of a plurality of holes with diameters smaller in size than a diameter of the at least one inlet hole, at least one outlet hole located on an outlet side of the microfluidic device opposite the inlet side; and a micro-channel, where the plurality of holes are connected to the micro-channel.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: March 7, 2023
    Assignee: Canon Virginia, Inc.
    Inventors: Yoichi Murakami, Makoto Ogusu, Christina Pysher, Scott Sundberg, Chris J Felice, David Li
  • Patent number: 11571635
    Abstract: The present invention relates to a liquid chromatography system for the separation of bio-molecules in a fluid including at least two unit operations, wherein the first unit operation is a step of multi-column chromatography and the second unit operation is a step modifying said bio-molecules and/or the fluid, wherein the modification comprises feeding the fluid resulting from the last chromatography column of the first unit operation into a system comprising at least two containers, wherein each container has a volume and a moveable sidewall arranged to divide the volume into a first sub-volume and a second sub-volume, and each container comprises a first port connected to the first volume and a second port connected to the second sub-volume. The invention also relates to a virus inactivation device for a chromatography system according to the invention, which enables continuous or semi-continuous processing of biomolecules, as well as a method of using such a device.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: February 7, 2023
    Assignee: Cytiva Sweden AB
    Inventors: Bjorn Markus Olovsson, Anders Ljunglof, Karol Maciej Lacki
  • Patent number: 11548000
    Abstract: Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: January 10, 2023
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Benjamin Wunsch, Joshua T. Smith, Stacey Gifford, Sung-Cheol Kim
  • Patent number: 11543415
    Abstract: Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
    Type: Grant
    Filed: June 3, 2019
    Date of Patent: January 3, 2023
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Yanni Zhang, Nigel J. Clarke, Richard E Reitz