Abstract: Restriction enzymes are used to remove from DNA a complete and undamaged structural gene coding region for the expression of DNA polymerase I (polA) without the gene's natural promoter or with only a significantly damaged portion of the gene's natural promoter. Also by the use of restriction enzymes, a segment from a plasmid cloning vector is excised at a position adjacent to a promoter which is conditionally controllable and may be more powerful than the damaged or removed promoter. The gene for DNA polymerase I is enzymatically cloned into said vector at the position of said removed segment and adjacent to said conditionally controllable promoter. Multicopies of the cloned vector are introduced into a host baterial strain (E. coli). The host strain is then cultured so that the cell colony grows and replicates new generations containing replicated foreign plasmid. During such said replication the activity of said controllable promoter is repressed.

Abstract: The present invention relates to a method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells (transportable proteins) by the addition of supernumerary N-linked oligosaccharide side chains at chosen sites on the surface thereof using oligonucleotide mutagenesis. The present invention also relates to mutant transportable proteins having supernumerary N-linked oligosaccharide side chains which shield functional sites or epitopes; and genes which encode the same.

Abstract: A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C.sub.5) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C.sub.5 to generate chain lengths of 10 to 11 nucleotides; longer products are also generated but at reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C.sub.5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template.

Abstract: Mammalian synovial phospholipase A.sub.2 (sPLA.sub.2) enzymes are provided, as well as DNA constructs encoding these enzymes, methods of producing the enzymes recombinantly, and antibodies thereto. Therapeutic methods employing anti-synovial phospholipase antibodies are also provided, in addition to diagnositc methods and other applications of sPLA.sub.2.

Abstract: A DNA sequence which encodes for a biologically active corn phosphoenolpyruvate carboxylase is disclosed. A recombinant plasmid vector containing the DNA sequence, a microorganism transformed with the vector, and the carboxylase expressed by the organism are also provided.