Abstract: A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.

Abstract: The .beta.-subunit of human nerve growth factor (.beta.NGF) is prepared in essentially pure form in commercially viable quantities using recombinant DNA technology. The nucleotide sequence and vectors encoding human .beta.NGF and host cells transformed with the vectors are also provided.

Abstract: It has been discovered that it is possible to target any RNA molecule for cleavage by RNase P by forming a hybrid region consisting of a short sequence of base pairs followed by a terminal 3'- NCCA sequence. In the preferred embodiment, the region is formed by addition of an external guide sequence consisting of a nucleotide sequence complementary to the targeted site which includes a 3'-NCCA, wherein the sequence hybridizes to the targeted RNA to form a short sequence of double-stranded RNA under conditions promoting cleavage of the substrate at the nucleotide at the 5' side of the base-paired region by the RNase P or catalytically active equivalent thereof. Specificity is determined by the complementary sequence. The sequence is preferably ten to fifteen nucleotides in length and may contain non-complementary nucleotides to the extent this does not interfere with formation of several base pairs followed by a NCCA at the 3' end.

Abstract: A DNA sequence is described, which is a promoter for Streptomyces. This promoter is stronger than the wild type and ultimately increases transcription initiation and protein expression. Typically, a nucleotide base, such as guanine, is inserted into the promoter sequence between positions -50 and -75 to increase transcription initiation or protein expression. In a preferred embodiment, guanine is inserted between positions -62 and -63 of the promoter regulated by thiostrepton (tipA).

Abstract: This invention is directed to a novel hybrid regulatory region for directing and regulating transcription and translation of a gene sequence positioned downstream from the hybrid regulatory region. This hybrid regulatory region includes the promoter sequence of the phage lambda P.sub.R promoter-operator region fused to the operator sequence of the phage lambda P.sub.L promoter-operator region.

Abstract: A method for preparing a ds DNA from a ss DNA template, which method includes:(a) providing a first DNA strand;(b) adding a homopolymeric oligonucleotide tail to the 3' end of the first DNA strand, to yield a tailed first DNA strand;(c) providing a ss homopolymeric oligonucleotide primer complementary to a portion of the tail;(d) contacting the primer with the tailed first DNA strand;(e) synthesizing, in the presence of the primer and the tailed first DNA strand, a second DNA strand complementary to the first DNA strand; and(f) removing the tail and the primer from the first and second DNA strands, respectively; provided that one or both of the tail and the primer contain(s) RNA.

Abstract: A plasmid and a DNA fragment each of which contains a gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium, and a coryneform bacterium containing said DNA fragment. The gene for tetracycline resistance derived from a glutamic acid-producing coryneform bacterium is a useful selective marker in effecting the breeding of a glutamic acid-producing coryneform bacterium by genetic recombination. Using the plasmid, the DNA fragment or the coryneform bacterium of the present invention, breeding of the glutamic acid-producing coryneform bacterium can be easily and effectively conducted by means of recombinant DNA technique.

Abstract: Methods for producing blunt-ended double stranded DNA, for labelling the 3'-end of a DNA fragment, and for in vitro mutagenesis of a DNA fragment. A processive DNA polymerase is used in each method.

Abstract: The present invention relates to the plasmid pTR2030 and derivatives thereof which confer phage resistance to group N streptococci. The invention further relates to microorganisms containing pTR2030 or a derivative thereof and to starter cultures containing the microorganisms.

Abstract: A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.

Abstract: A two-step method for cloning the BamHI restriction modification system is provided which comprises introducing the BamHI modification or methylase gene into a host cell and expressing the gene to protect the host cell followed by introducing the BamHI restriction or endonuclease gene into the host.

Abstract: A bacteriophage identified as .phi.Ac1 capable of infecting acidophilic heterotropic bacteria (such as Acidiphilium sp.) and processes for genetically engineering acidophilic bacteria for biomining or sulfur removal from coal are disclosed. The bacteriophage is capable of growth in cells existing at pH at or below 3.0. Lytic forms of the phage introduced into areas experiencing acid drainage kill the bacteria causing such drainage. Lysogenic forms of the phase having genes for selective removal of metallic or nonmetallic elements can be introduced into acidophilic bacteria to effect removal of the desired element form ore or coal.

Abstract: A human erythroid specific enhancer element is described. The enhancer element can be used to enhance transcription of a structural gene in erythroid cells. Methods for gene therapy employing the enhancer element are also disclosed.

Abstract: A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of the enzyme glucose dehydrogenase, and a new DNA sequence, recombinant DNA vector, and transformed microorganism therefor.

Abstract: Expression systems which utilize bacteriophage T7 RNA mutant polymerases and promoter regions are provided by this invention. These systems are utilized to produce large quantities of useful polypeptides and proteins.

Abstract: New RNA endoribonuclease ribozymes are found with new conditions to prevent mismatch cleavage and able to cleave RNA after 6 different sets of ribonucleotide 4 base sequences.

Abstract: The invention provides DNA fragments coding for human apolipoprotein E or human apolipoprotein E-like substances having physiological activities equivalent to those of said human apolipoprotein E, expression vectors suitable for production of such proteins, hosts for use in the production, and process for the production, as well as such proteins thereby produced.

Abstract: A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

Abstract: A method of producing and selectively isolating a desired protein or polypeptide or derivative thereof by constructing a recombinant vector comprising a DNA sequence coding for said desired protein or polypeptide operatively linked to a DNA sequence coding for protein A or an active polypeptide fragment thereof or any other macromolecule capable of binding to the constant regions of immunoglobulins, such that said DNA sequences together code for an IgG-binding fusion product between said desired protein or polypeptide and said protein A, active polypeptide fragment thereof or macromolecule; transforming a compatible host with said recombinant vector such that the combined DNA sequences coding for said fusion protein or polypeptide can be expressed by the host, and culturing the transformed host in a suitable growth medium to produce said fusion protein or polypeptide; selectively isolating said fusion protein or polypeptide by adsorption to an IgG-supporting carrier material; and optionally desorbing said fus

Abstract: RNA enzymes or ribozymes can act as endoribonucleases, catalyzing the cleavage of RNA molecules with a sequence specificity of cleavage greater than that of known ribonucleases and approaching that of the DNA restriction endonucleases, thus serving as RNA sequence specific endoribonucleases. An example is a shortened form of the self-splicing ribonsomal RNA intervening sequence of Tetrahymena (L-19 IVS RNA). Site-specific mutagenesis of the enzyme active site of the L-19 IVS RNA alters the substrate sequence specificity in a predictable manner, allowing a set of sequence-specific endoribonucleases to be synthesized. Varying conditions allow the ribozyme to act as a polymerase (nucleotidyltransferase), a dephosphorylase (acid phosphatase or phosphotransferase) or a sequence-specific endoribonuclease.