Patents Examined by Robert B. Mondesi
  • Patent number: 11015178
    Abstract: The present invention is related to a method for adding one or more L-nucleotides to the 3? end of a first L-nucleic acid, wherein the method comprises the step of reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising an enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more L-nucleotides to the 3? end of the first L-nucleic acid.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: May 25, 2021
    Assignee: APTARION biotech AG
    Inventors: Andreas Pech, Ralf David, Florian Jarosch, Michael Jahnz, Sven Klussmann
  • Patent number: 11008534
    Abstract: A bacteria referred to here as Bacillus subtilis 6A-1 is provided, compositions thereof and processes for use of the bacteria, spores, cells, extracts and enzymes. The compositions which comprise the bacteria, spores, cells, extracts and/or enzymes are capable of degrading polysaccharides. Such compositions are capable of degrading cellulose, including plant-produced cellulose, microcrystalline cellulose and carboxymethyl cellulose. The bacteria produces at least two cellulose-degrading protein fractions. Cellulose degrading activity continues across pH2 to pH13.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: May 18, 2021
    Assignee: AGRI-KING, Inc.
    Inventors: Gbenga Ayangbile, Mary Grzemski, James F. Tobey, Jr., David Spangler, Lucas Krueger
  • Patent number: 10995321
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 10995322
    Abstract: Genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids utilizing genetically modified isopropylmalate synthases, and microbial organisms including genetically modified isopropylmalate synthases are described. The genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids, and microbial organisms including genetically modified isopropylmalate synthases can be particularly useful for producing corresponding Cn_1 aldehydes, alcohols, carboxylic acids, and Cn_2 alkanes both in vivo and in vitro.
    Type: Grant
    Filed: December 30, 2016
    Date of Patent: May 4, 2021
    Assignee: Dow Global Technologies LLC
    Inventors: Paresh C. Sanghani, Sarah Delaplane
  • Patent number: 10995350
    Abstract: Provided herein are microorganisms that include one or more heterologous nucleic acid selected from the group of a sequence encoding a 7-methylxanthosine synthase, a sequence encoding a theobromine synthase; and a sequence encoding a caffeine synthase, where the microorganism is capable of producing one or more purine alkaloid in a culture medium, when the microorganism is cultured under conditions sufficient to produce the one or more purine alkaloid. Also provided compositions and kits that include at least one of these microorganisms, and methods of producing one or more purine alkaloid that include culturing one of these microorganisms under conditions sufficient to produce the one or more purine alkaloid.
    Type: Grant
    Filed: March 22, 2017
    Date of Patent: May 4, 2021
    Assignee: MOgene Green Chemicals LLC
    Inventors: Jana Stockel, Abhay Kumar Singh, Himadri Pakrasi, Ganesh Murthy Kishore
  • Patent number: 10995320
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: December 12, 2019
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Patent number: 10988739
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: December 9, 2019
    Date of Patent: April 27, 2021
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 10982244
    Abstract: The present disclosure relates to modified homoserine dehydrogenase and a method for producing homoserine or a homoserine-derived L-amino acid using the same.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: April 20, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Su Yon Kwon, Kwang Woo Lee, Lan Huh, Kyungrim Kim, Mina Baek, Seung-ju Son, Jaemin Lee
  • Patent number: 10975400
    Abstract: A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: April 13, 2021
    Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
    Inventors: Ping Zheng, Jiuzhou Chen, Wei Pu, Jibin Sun, Xinyang Wu, Yanhe Ma
  • Patent number: 10975406
    Abstract: The invention provides compositions and methods for repeatable directed endonucleases (RDEs) and methods for repeatedly, and specifically cleaving DNA offset from the RDE's DNA recognition sequence on the target nucleic acid rather than within the DNA recognition sequence. Conservation of the recognition sequence of the target nucleic acid enables for re-localization of an RDE back to the DNA recognition sequence for further cleavage. The RDEs and methods of the invention are useful in applications including, but not limited to, recording data into a genome, timing the order of biochemical pathway events, efficient genome engineering and encoding lagged cellular death.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: April 13, 2021
    Assignee: Massachusetts Institute of Technology
    Inventors: Joseph M. Jacobson, Noah Michael Jakimo
  • Patent number: 10975403
    Abstract: The present invention relates to the production of eriodictyol via bioconversion.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: April 13, 2021
    Assignee: Conagen Inc.
    Inventors: Rui Zhou, Xiaodan Yu, Steven Chen
  • Patent number: 10968437
    Abstract: Factors For the Production and Accumulation of Polyunsaturated Fatty Acids (PUFAs) Derived from PUFA Synthases Abstract Disclosed are novel enhancing factor proteins of the PUFA synthase systems, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified microorganisms comprising the same, and methods of making and using the same. Also disclosed are genetically modified microorganisms that have been genetically modified to express a PUFA synthase system for the production of PUFAs, wherein the microorganisms have been modified to express the novel enhancing factor proteins of the PUFA synthase system.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: April 6, 2021
    Assignee: DSM IP Assets B.V.
    Inventors: James George Metz, Jerry M. Kuner, David Glen McCaskill, Mendy Louise Foster
  • Patent number: 10961554
    Abstract: The present disclosure relates to a novel promoter and a method for producing L-amino acids using the promoter, and more specifically, to a novel polynucleotide having promoter activity, a vector and a microorganism of the genus Corynebacterium comprising the polynucleotide, a method for producing L-amino acids and a fermented composition using the microorganism, and a fermented composition.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: March 30, 2021
    Assignee: CJ Cheiljedang Corporation
    Inventors: Ji Yeon Lee, Jin Sook Chang, Hyung Joon Kim, Byoung Hoon Yoon, Sun Hyoung Choi, Yunjung Choi
  • Patent number: 10954543
    Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
    Type: Grant
    Filed: November 2, 2016
    Date of Patent: March 23, 2021
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Mattheos Koffas, John Andrew Jones
  • Patent number: 10941424
    Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: March 9, 2021
    Assignee: BRASKEM S.A.
    Inventors: Daniel Johannes Koch, Mateus Schreiner Lopes, Ane Fernanda Beraldi Zeidler, Lucas Pedersen Parizzi
  • Patent number: 10941429
    Abstract: Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
    Type: Grant
    Filed: January 16, 2019
    Date of Patent: March 9, 2021
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Tomás Pluskal, Jing-Ke Weng
  • Patent number: 10927389
    Abstract: A method of producing a chemical product by continuous fermentation utilizes a separation membrane under conditions at a pH of not more than 3.5, wherein yeast having vanillin resistance is used to enable efficient production of a chemical product without leaving a large amount of fermentation feedstock unused, is provided.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: February 23, 2021
    Assignee: Toray Industries, Inc.
    Inventors: Koji Kobayashi, Kenji Sawai, Katsushige Yamada
  • Patent number: 10927353
    Abstract: Use of a catalyst in a method of reducing a substrate, the method comprising contacting a substrate with a catalyst, optionally in the presence of a co-substrate, thereby to generate a reduced substrate. The catalyst is a polypeptide comprising an amino acid sequence having at least 70% identity to SEQ ID NO: 1, SEQ ID NO: 7 or SEQ ID NO: 9. In the method the substrate concentration is at least 50 mM.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: February 23, 2021
    Assignee: Johnson Matthey Public Limited Company
    Inventors: Beatriz Dominguez, Ursula Schell, Christian Kratzer, Thomas Kalthoff
  • Patent number: 10927356
    Abstract: The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: February 23, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Luise Erlandsen, Carsten Hoerslev Hansen, Jesper Vind, Allan Svendsen, Carsten Peter Sonksen
  • Patent number: 10927352
    Abstract: Use of a catalyst in a method of reducing a substrate, the method comprising contacting a substrate with a catalyst, optionally in the presence of a co-substrate, thereby to generate a reduced substrate. The catalyst is a polypeptide comprising an amino acid sequence having at least 70% identity to SEQ ID NO: 7. In some methods, the substrate concentration is at least 50 mM.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: February 23, 2021
    Assignee: Johnson Matthey Public Limited Company
    Inventors: Beatriz Dominguez, Ursula Schell, Christian Kratzer, Thomas Kalthoff