Abstract: The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases. Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects. Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.
Type:
Grant
Filed:
February 19, 2019
Date of Patent:
January 4, 2022
Assignee:
Synthetic Biologies, Inc.
Inventors:
Pertti Koski, Ulla Airaksinen, Katja Valimaki
Abstract: Provided are phytase mutants, preparation methods therefor and uses thereof, DNA molecule encoding each of the phytase mutants, a vector comprising the DNA molecule, and a host cell comprising the vector.
Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
Type:
Grant
Filed:
May 11, 2020
Date of Patent:
January 4, 2022
Assignee:
California Institute of Technology
Inventors:
Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
Abstract: The present invention concerns a detergent and a pharmaceutical composition comprising a deoxyribonuclease (DNase), wherein the DNase is obtained from a fungal source. It further concerns a laundering method and the use of DNase. The present invention further relates to polypeptides having DNase activity, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides.
Type:
Grant
Filed:
October 9, 2018
Date of Patent:
January 4, 2022
Assignee:
NOVOZYMES A/S
Inventors:
Klaus Gori, Marie Allesen-Holm, Lilian Eva Tang Baltsen, Allan Noergaard, Jan Lehmbeck
Abstract: The invention provides methods for rapid and quantitative extraction and detection of coenzyme Q10 in a sample readily adaptable to high throughput screening methods. The invention further provides reagents and kits for practicing the methods of the invention.
Abstract: The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.
Type:
Grant
Filed:
April 7, 2020
Date of Patent:
December 21, 2021
Assignee:
The Regents of the University of California
Inventors:
John Eugene Dueber, Zachary Nicholas Russ, Tammy Melody Hsu, Terry Don Johnson, Jr., Bernardo Cervantes, Ramya Lakshmi Prathuri, Shyam Pravin Bhakta, Arthur Muir Fong, III, Luke Nathaniel Latimer
Abstract: The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from Leuconostoc mesenteroides, and subjected to site-directed mutagenesis to improve the enzyme activity of sucrose phosphorylase. The mutant is expressed in Corynebacterium glutamicum and used as a whole cell catalyst to produce 2-O-?-D-glycerol glucoside. At a 5 L fermentation tank level, a large quantity of 2-O-?-D-glycerol glucoside can be produced efficiently in a short time, which is conducive to expanding the prospect of industrial application of sucrose phosphorylase for the production of 2-O-?-D-glycerol glucoside and realizing its large-scale industrial application.
Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.
Type:
Grant
Filed:
June 14, 2019
Date of Patent:
December 14, 2021
Assignee:
Codexis, Inc.
Inventors:
Scott J. Novick, Ravi David Garcia, Charlene Ching, Nikki Dellas, David Entwistle, Vesna Mitchell
Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
December 18, 2015
Date of Patent:
December 14, 2021
Assignee:
NOVOZYMES A/S
Inventors:
Frank W. Rasmussen, Miguel Duarte Guilherme Pereira Toscano, Lars L. H. Christensen, Esben Peter Friis
Abstract: Disclosed herein are compositions and methods of treating disclosure provides for compounds for use in treating Mitochondrial Neurogastrointestinal Encephalopathy Syndrome (MNGIE). In some embodiments, the compounds have cell penetrating activity and thymidine phosphorylase activity. In certain embodiments, the compounds disclosed herein comprise: a) at least one cell-penetrating peptide (CPP) moiety; and b) a thymidine phosphorylase, or an active fragment or analog thereof (TP), wherein the CPP is coupled, directly or indirectly, to TP.
Type:
Grant
Filed:
March 17, 2020
Date of Patent:
November 9, 2021
Assignee:
Entrada Therapeutics, Inc.
Inventors:
Natarajan Sethuraman, Jason Ruth, Lou A. Tartaglia, Dehua Pei, Ziqing Qian
Abstract: The present invention relates generally to an eco-friendly methodology for the conversion of alcohols and aldehydes to amines and amides using an integrated enzyme cascade system with metal- and organocatalysis. More specifically, the present invention relates to synthesis of capsaicinoids starting from vanillin alcohol and using a combination of an enzyme cascade system and catalysts. Furthermore, the method also relates to synthesis of capsaicinoids derivatives starting from vanillin alcohol derivatives and using a combination of an enzyme cascade system and catalysts.
Type:
Grant
Filed:
March 27, 2015
Date of Patent:
November 9, 2021
Assignee:
XP Chemistries AB
Inventors:
Armando Córdova, Per Berglund, Mattias Anderson, Samson Afewerki
Abstract: The current disclosure provides a process for enzymatically converting a saccharide into allulose. The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase (A6PP).
Type:
Grant
Filed:
December 4, 2020
Date of Patent:
November 9, 2021
Assignee:
BONUMOSE, INC.
Inventors:
Daniel Joseph Wichelecki, Edwin O. Rogers
Abstract: A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.
Type:
Grant
Filed:
January 22, 2018
Date of Patent:
November 2, 2021
Assignee:
JILIN ASYMCHEM LABORATORIES CO., LTD.
Inventors:
Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Na Zhang, Yulei Ma, Yibing Cheng, Huiyan Mu
Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.
Abstract: The present invention provides a method for the production of oligosaccharides by the fermentation of dextran-sucrase-producing microorganisms with sucrose and maltose. The disclosed process allows for the control of the final composition of the isomaltooligosaccharides by adjustments to pH and the initial ratio of sucrose to maltose.
Type:
Grant
Filed:
August 22, 2015
Date of Patent:
October 26, 2021
Assignee:
ISOThrive Inc.
Inventors:
Lee Madsen, II, Jack Oswald, Donal F Day, Young Hwan Moon
Abstract: A method for producing an objective substance such as sphingoid bases and sphingolipids using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance in a culture medium containing an additive that is able to associate with, bind to, solubilize, and/or capture the objective substance, and collecting the objective substance from cells of the yeast and/or the culture medium.
Type:
Grant
Filed:
February 16, 2018
Date of Patent:
October 26, 2021
Assignees:
Ajinomoto Co., Inc., Evolva SA
Inventors:
Markus Schwab, Corina Daniela Wirdnam, Thomas Oestergaard Tange, Maud Babau, Anaelle Hatsch, Fanny Delegrange, David Fischer, Sabina de Andrade Pereira Tavares
Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
Type:
Grant
Filed:
February 26, 2020
Date of Patent:
October 12, 2021
Assignee:
Genomatica, Inc.
Inventors:
Derek L. Greenfield, Donald E. Trimbur, Andreas W. Schirmer, Cindy Chang, Behnaz Behrouzian, Jessica Winger
Abstract: Disclosed herein are methods that may be used for the synthesis of benzylisoquinoline alkaloids (BIAs) such as alkaloid morphinan. The methods disclosed can be used to produce thebaine, oripavine, codeine, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone, naltrexone, naloxone, hydroxycodeinone, neopinone, and/or buprenorphine. Compositions and organisms useful for the synthesis of BIAs, including thebaine synthesis polypeptides, purine permeases, and polynucleotides encoding the same, are provided.
Type:
Grant
Filed:
June 27, 2017
Date of Patent:
October 12, 2021
Assignee:
Antheia, Inc.
Inventors:
Peter James Facchini, Xue Chen, Jeffrey C. Colbeck, Joseph Tucker
Abstract: Disclosed in the present invention is a recombinant Escherichia coli and application thereof. The recombinant Escherichia coli was deposited on Aug. 28, 2018 in the China General Microbiological Culture Collection Center with the preservation number CGMCC No. 16349. The recombinant Escherichia coli is fermented and cultured, and the concentration of Microcin J25 in the supernatant can reach 4000 mg/L or more.
Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
Type:
Grant
Filed:
October 3, 2016
Date of Patent:
October 5, 2021
Assignee:
DSM IP Assets B.V.
Inventors:
Ioannis Papapetridis, Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Paul Klaassen