Abstract: A method for selective cleavage of C—N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C—N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C—N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C—N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.

Abstract: Proteins which have activity as anticoagulants and/or serine protease inhibitors and have at least one NAP domain and are described. Certain of these proteins have factor Xa inhibitory activity and others have activity as inhibitors of factor VIIa/TF. These proteins can be isolated from natural sources as nematodes, chemically synthesized or made by recombinant methods using various DNA expression systems.

Abstract: This invention relates generally to an assay for Lyme disease which detects the antibody to Borrelia burgdorferi, the causative agent of Lyme disease. More specifically, the assay employs antigens derived from amino acid regions in the flagellum of Borrelia burgdorferi. These antigens are immunoreactive with antibodies to Borrelia burgdorferi but are not substantially immunoreactive with antibodies to Treponema pallidum, the syphilis causing agent. DNA sequences of the antigens, clones and vectors containing the DNA sequences are also disclosed. Polypeptides derived therefrom can be used as reagents for the detection of antibody to Borrelia burgdorferi in the body fluids from individuals with Lyme disease.

Abstract: A synthetic DNA sequence and its genetic equivalents are disclosed which sequences are capable, when used in a recombinant DNA method, of directing production of a serine protease inibitor protein. Recombinant DNA methods for the production of serine protease inhibitor proteins are also disclosed. These methods incorporate either the synthetic DNA sequence of the present invention or natural DNA sequences isolated from human cDNA or genomic libraries.

Abstract: The present invention is directed to the production of an antipathogenic substance (APS) in a host via recombinant expression of the polypeptides needed to biologically synthesize the APS. Genes encoding polypeptides necessary to produce particular antipathogenic substances are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize any desired APS. The cloned genes may be transformed and expressed in a desired host organisms to produce the APS according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of the APS.

Abstract: A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.

Abstract: A substantially pure non-glycosylated human interleukin-2 protein having a specific activity of not less than 10.sup.4 U/mg is obtained by growing a transformant carrying a DNA having a base sequence coding for human interleukin-2 to cause production and accumulation of human interleukin-2 in the culture broth, subjecting the thus obtained human interleukin-2-containing liquid to a purification process comprising a hydrophobic column chromatography.

Abstract: Novel lipolytic enzymes having an optimum pH in the range of 8 and 10.5 which exhibits effective lipase activity in an aqueous solution containing a detergent at a concentration of up to about 10 g/l under washing conditions at a temperature of 60.degree. C. or below and at a pH between 7 and 11. These lipases are useful in washing compsitions, premixes and enzymatic detergent additives. They are obtainable from bacteria strains selected from Psedomonas pseudoalcaligenes, Pseudomanas stutzeri and Acinetobacter calcoaceticus. Preferred strains are Pxs. pseudoalcaligenes CBS 467.85, CBS 468.85, CBS 471.85, CBS 473.85 and ATCC 29625, Ps. stutzeri CBS 461.85 and Acimetobacter calcoaceticus CBS 460.85, and variants and mutants thereof.

Abstract: A method for modifying eukaryotic and prokaryotic proteins to extend their in vivo circulatory lifetimes. In the preferred embodiment, enzymatic and/or chemical treatments are used to produce a modified protein carrying one or more covalently attached trisaccharide, sialic acid.fwdarw.galactose.fwdarw.N-acetylglucosamine.fwdarw.(SA.fwdarw.Gal.fwd arw.GlcNac.fwdarw.), or tetrasaccharide (SA.fwdarw.Gal.fwdarw.GlcNAc.fwdarw.GlcNAc.fwdarw.) moieties. The method can be applied to any natural or recombinant protein possessing asparagine-linked oligosaccharides or to any non-glycosylated protein that can be chemically or enzymatically derivatized with the appropriate carbohydrate units. Following injection into an animal, the modified glycoproteins are protected from premature clearance by cells of the liver and reticulo-endothelial system which recognize and rapidly internalize circulating glycoproteins with carbohydrate chains containing terminal Gal, GlnNAc, fucose or mannose residues.

Abstract: A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the cabohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, the .alpha. or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligosaccharide compound being separated from the reaction mixture.

Abstract: A process is provided for treating aqueous carbohydrate solutions with phospholipase enzyme compositions to improve the filterability and clarity of the filtrate of such solutions.

Abstract: Compositions which comprise a calcium magnesium acetate double salt are useful in deicing compositions. Deicing compositions comprising those compositions and processes for their preparation are described.

Abstract: The preparation of aminoalkyl phenyl selenides and pharmaceutically acceptable salts thereof is disclosed which are useful for the treatment of hypertension and related vascular diseases and the treatment of nervous system dysfunctions.

Abstract: A enzyme-containing solid composition is prepared by a process comprising removing the solvent from a mixture of a water-soluble enzyme, a water-insoluble metal salt of a fatty acid and an organic solvent.

Abstract: Thermally stable polyoxyethylene polyethers prepared by oxyethylating aromatic initiators having at least two reactive hydrogens derived from amino or hydroxyl functional groups located para to each other are disclosed. These polyethers are especially suitable as solder bath oils, solder reflow oils, and heat transfer fluids for high temperature applications.

Abstract: An electrically conductive composite comprising conductive polyacetylene moiety, a nonconductive high nitrile resin, and a dopant. The high nitrile resin further comprises at least nitrile monomers or comonomers and optionally copolymerized with comonomers of mono-ethylenically unsaturated monomers and conjugated diolefins and further optionally containing an elastomeric component.The invention further includes a process for producing an electrically conductive composite comprising:(1) impregnating a high nitrile resin with a Zeigler type catalyst comprising an alkyl aluminum compound and alkyl, alkyl halide, halide, oxyhalide or alkoxide of Group IVA and VA metals,(2) exposing the impregnated high nitrile resin with an alkyne under polymerization conditions whereby polymerization occurs to form a polyacetylene/nitrile composite, and(3) adding dopant to the composite.

Abstract: A collector composition for use in froth flotation processes comprises two collectors. One of the collectors is preferably an N-(hydrocarbyl)-alpha, omega-alkanediamine, an (omega-aminoalkyl) hydrocarbon amide or mixture thereof. The second collector is a thiocarbonate, a thionocarbamate, a thiophosphate, thiocarbinilide, thiophosphinate, mercaptan, xanthogen formate, xanthic ester or mixture thereof. The collector composition floats a broad range of metal-containing minerals.

Abstract: A method and cleaning composition are the subject of the invention. The cleaning composition may take the form of an aqueous solution, a powder, or a concentrated powder or aqueous solution for addition to a cleaning solution. The additive includes an amine or polyacrylamide flocculant and a betaine stabilizer. When used in a detergent solution, the flocculant causes contaminates introduced into the water to precipitate and quickly settle to the bottom of the cleaning bucket. This keeps the upper part of the detergent solution clean and prevents the mop from picking up and subsequently redepositing the contaminates each time it is dipped in the bucket.

Abstract: The present invention relates to a class of compounds which have superior soil release properties over heretofore known soil release polymers. The compounds are prepared by the reaction of an aromatic hydroxy containing polyester soil release agent with trimellitic anhydride under acid catalysis to produce an aromatic terminal capped carbonyloxy soil release polymer.

Abstract: An antiscalant admixture is disclosed of a homopolymer of maleic acid or a copolymer and a phosphonoalkane carboxylic acid for reducing scaling in an aqueous system wherein the weight ratio of said copolymer to said phosphonic acid is 5:1 to 1:5, wherein molecular weight of said copolymer is 2,000 to 20,000, and wherein said polymer is composed of at least two of the comonomers selected from monounsaturated monocarboxylic and dicarboxylic acids of 3 to 5 carbon atoms, water-soluble salts thereof, and alkyl esters thereof of 1 to 6 carbon atoms in said alkyl groups; substituted acrylamides; acrylamidoalkane sulfonic acids and water-soluble salts thereof; vinyl sulfonic acid and water-soluble salts thereof; hydroxyalkyl acrylates with 2 to 4 carbons in the alkylene group; styrene sulfonic acids and water-soluble salts thereof; allyloxyhydroxyalkane sulfonic acids and water-soluble salts; vinyl carboxylates; polymerized vinyl alcohols and water-soluble salts thereof; and mixtures of such comonomers.