Abstract: The present invention includes a closed sterile in-vitro fertilization system to provide incubation for an oocyte. The system comprises a pouch having a flexible front and a flexible back wall joined at their outer peripheries. The pouch is divided into a first and a second segregated chamber. An entry port is arranged in each of the chambers through the wall of the pouch. A vacuum means is arranged in the first chamber, to permit direct aspiration of an oocyte from an ovary into that chamber, and an entry conduit is arranged between the first and the second chambers, to permit an oocyte to be transferred therebetween.