Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Abstract: The present invention provides a novel gene WMS conferring wheat male sterility, its anther-specific expression promoter, and uses of the same. In wheat, a well-known gene Ms2 causing dominant male sterility has been widely applied in recurrent selection in China. A RNA-seq approach was performed to reveal the anther-specific transcriptome in a pair of Ms2 isogenic lines, ‘Lumai 15’ and ‘Lumai 15+Ms2’. As a result, a WMS gene was identified showing anther-specific expression at the early stage of meiosis and only in wheat carrying the Ms2 gene. The regulation of WMS could alter plant male fertility. The promoter of WMS was found to comprise anther-specific activity. Thus, the present invention might be used to achieve anther-specific gene expression, to develop male sterility in various plant species, to establish recurrent selection in various plant species, and to assist hybrid seed production.

Abstract: A novel soybean variety, designated 5PWYY47 is provided. Also provided are the seeds of soybean variety 5PWYY47, cells from soybean variety 5PWYY47, plants of soybean 5PWYY470, and plant parts of soybean variety 5PWYY47. Methods provided include producing a soybean plant by crossing soybean variety 5PWYY47 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PWYY47, methods for producing other soybean varieties or plant parts derived from soybean variety 5PWYY47, and methods of characterizing soybean variety 5PWYY47. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PWYY47 are further provided.

Abstract: A novel soybean variety, designated 5PZUG30 is provided. Also provided are the seeds of soybean variety 5PZUG30, cells from soybean variety 5PZUG30, plants of soybean 5PZUG30, and plant parts of soybean variety 5PZUG30. Methods provided include producing a soybean plant by crossing soybean variety 5PZUG30 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PZUG30, methods for producing other soybean varieties or plant parts derived from soybean variety 5PZUG30, and methods of characterizing soybean variety 5PZUG30. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PZUG30 are further provided.

Abstract: Embodiments of the present invention provide for a transgenic plan, methods of making and DNA constructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a/b ratio at high light intensity and a decrease in the Chl a/b ratio at low light intensity versus wild-type plants grown in the same conditions.

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

Abstract: A novel soybean variety, designated 5PJQV48 is provided. Also provided are the seeds of soybean variety 5PJQV48, cells from soybean variety 5PJQV48, plants of soybean 5PJQV48, and plant parts of soybean variety 5PJQV48. Methods provided include producing a soybean plant by crossing soybean variety 5PJQV48 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PJQV48, methods for producing other soybean varieties or plant parts derived from soybean variety 5PJQV48, and methods of characterizing soybean variety 5PJQV48. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PJQV48 are further provided.

Abstract: Methods to produce cannabis plants are described, the methods include growing the cannabis plants in a growing medium within an interior of an enclosure by providing a common reservoir including water, and transferring the water from the common reservoir to the cannabis plants within the interior of the enclosure, the water within the common reservoir includes, fish, treated water, evaporator condensate, and a microorganism. Methods to asexually clone, harvest, trim, grind, and heat the cannabis plants are also described.

Abstract: A novel soybean variety, designated 5PCVB54 is provided. Also provided are the seeds of soybean variety 5PCVB54, cells from soybean variety 5PCVB54, plants of soybean 5PCVB54, and plant parts of soybean variety 5PCVB54. Methods provided include producing a soybean plant by crossing soybean variety 5PCVB54 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PCVB54, methods for producing other soybean varieties or plant parts derived from soybean variety 5PCVB54, and methods of characterizing soybean variety 5PCVB54. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PCVB54 are further provided.

Abstract: The present disclosure provides, a DNA construct comprising a polynucleotide fragment comprising a first, and a second sequence, wherein said first sequence encodes a mitochondrial transit peptide, said second sequence encodes a polypeptide having amino acid sequence as set forth in SEQ ID NO: 1, and said polynucleotide fragment is operably linked to a flower specific promoter. The present disclosure also provides with a DNA vector, a recombinant host cell and a method of obtaining the same.

Abstract: A breeding method of a polyploid rice photo-thermo-sensitive genetic male sterile line includes determining a diploid rice line with photo-thermo-sensitive genetic male sterility or PMeS characteristic as a parent; carrying out hybridization on a diploid photo-thermo-sensitive genetic male sterile line and a diploid PMeS gene line, carrying out doubling culture on a young ear of a hybrid plant into a hybrid tetraploid; back-crossing the hybrid tetraploid with a tetraploid photo-thermo-sensitive genetic male sterile line; selecting a tetraploid male sterile plants from the back-crossed progeny, self-crossing during a low-temperature and short-day fertile period, and then carrying out composite hybridization with another tetraploid rice line having PMeS gene; selecting tetraploid male sterile plants, and detecting the stability of tetraploid male sterile plants after multiple generations of continuous self-crossing; and determining the stable and consistent tetraploid rice sterile line as the polyploid rice pho

Abstract: A soybean cultivar designated 88482541 is disclosed. The invention relates to the seeds of soybean cultivar 88482541, to the plants of soybean cultivar 88482541, to the plant parts of soybean cultivar 88482541, and to methods for producing progeny of soybean cultivar 88482541. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 88482541. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 88482541, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 88482541 with another soybean cultivar.

Abstract: A soybean cultivar designated 84344663 is disclosed. The invention relates to the seeds of soybean cultivar 84344663, to the plants of soybean cultivar 84344663, to the plant parts of soybean cultivar 84344663, and to methods for producing progeny of soybean cultivar 84344663. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 84344663. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 84344663, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 84344663 with another soybean cultivar.

Abstract: A soybean cultivar designated 83292541 is disclosed. The invention relates to the seeds of soybean cultivar 83292541, to the plants of soybean cultivar 83292541, to the plant parts of soybean cultivar 83292541, and to methods for producing progeny of soybean cultivar 83292541. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 83292541. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 83292541, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 83292541 with another soybean cultivar.

Abstract: A soybean cultivar designated 84340383 is disclosed. The invention relates to the seeds of soybean cultivar 84340383, to the plants of soybean cultivar 84340383, to the plant parts of soybean cultivar 84340383, and to methods for producing progeny of soybean cultivar 84340383. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 84340383. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 84340383, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 84340383 with another soybean cultivar.

Abstract: A soybean cultivar designated 81322943 is disclosed. The invention relates to the seeds of soybean cultivar 81322943, to the plants of soybean cultivar 81322943, to the plant parts of soybean cultivar 81322943, and to methods for producing progeny of soybean cultivar 81322943. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 81322943. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 81322943, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 81322943 with another soybean cultivar.

Abstract: A breeding method of a polyploid rice two-line restorer line includes determining hybrid parents for breeding the restorer line; breeding a restorer line of an indica sterile/japonica restorer type, or breeding a restorer line of a japonica sterile/indica restorer type, and carrying out backcrossing or composite hybridization after hybridization of the parents; selecting a single plant that meets the breeding goal, carrying out composite hybridization again and carrying out preliminary molecular marker detection; comparing, and selecting a single plant with good traits for continuous selfing until the line is basically stable; selecting a stable line with good traits and detection molecular markers; carrying out test-crossing on the preferred line as a male parent with different types of polyploid sterile lines; and selecting a good hybrid combination and a restorer line thereof.

Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

Abstract: Disclosed herein are methods for the large-scale production of a highly thermally stable acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Additionally, the expression methods disclosed herein can produce ChE preparations consisting of extract or purified forms that can be produced in high amounts and are highly thermally stable. These ChE products can be used in vitro detection, detoxification and decontamination methods.

Abstract: Provided are heterologous nucleic acid constructs, vectors and methods for elevating cyclic electron transfer activity, improving carbon concentration, and enhancing carbon fixation in C3 and C4 plants, and algae, and producing biomass or other products from C3 or C4 plants, and algae, selected from among, for example, starches, oils, fatty acids, lipids, cellulose or other carbohydrates, alcohols, sugars, nutraceuticals, pharmaceuticals, fragrance and flavoring compounds, and organic acids, as well as transgenic plants produced thereby. These methods and transgenic plants and algae encompass the expression, or overexpression, of various combinations of genes that improve carbon concentrating systems in plants and algae, such as bicarbonate transport proteins, carbonic anhydrase, light driven proton pump, cyclic electron flow regulators, etc.